Abstract
Objective: To test the hypothesis that activin A promotes in vitro human oocyte meiotic maturation while inhibiting steroid secretion by nonluteinized antral granulosa cells. Design: Prospective randomized controlled study. Setting: A university medical center. Patient(s): Nine women ranging in age from 31-44 years who were undergoing oophorectomy for nonovarian pathology. Intervention(s): Analysis of meiotic maturation of oocytes and steroid secretion by granulosa cells cultured in the presence or absence of activin A. Main OUtcome Measure(s): Germinal vesicle breakdown (GVBD) and attainment of metaphase II (MII) in oocytes, and progesterone and E2 secretion by granulosa cells. Result(s): Activin A significantly enhanced GVBD (91% vs. 65%) for control and maturation to MII (56% vs. 35% for control) of immature oocytes. Activin A significantly suppressed basal, and inhibin A-and FSH- stimulated progesterone and E2 secretion by nonluteinized granulosa cells. Conclusion(s): Activin A is a promoter of oocyte maturation in vitro and a modulator of granulosa cell steroidogenesis in culture.
Original language | English (US) |
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Pages (from-to) | 1126-1130 |
Number of pages | 5 |
Journal | Fertility and sterility |
Volume | 70 |
Issue number | 6 |
DOIs | |
State | Published - Dec 1998 |
Externally published | Yes |
Keywords
- Activin
- Estradiol
- Granulosa cells
- In vitro maturation
- Inhibin
- Oocyte
- Progesterone
ASJC Scopus subject areas
- Reproductive Medicine
- Obstetrics and Gynecology