Activin A stimulates meiotic maturation of human oocytes and modulates granulosa cell steroidogenesis in vitro

Baha M. Alak, Serdar Coskun, Chad I. Friedman, Elizabeth A. Kennard, Moon H. Kim, David Seifer

Research output: Contribution to journalArticle

83 Citations (Scopus)

Abstract

Objective: To test the hypothesis that activin A promotes in vitro human oocyte meiotic maturation while inhibiting steroid secretion by nonluteinized antral granulosa cells. Design: Prospective randomized controlled study. Setting: A university medical center. Patient(s): Nine women ranging in age from 31-44 years who were undergoing oophorectomy for nonovarian pathology. Intervention(s): Analysis of meiotic maturation of oocytes and steroid secretion by granulosa cells cultured in the presence or absence of activin A. Main OUtcome Measure(s): Germinal vesicle breakdown (GVBD) and attainment of metaphase II (MII) in oocytes, and progesterone and E2 secretion by granulosa cells. Result(s): Activin A significantly enhanced GVBD (91% vs. 65%) for control and maturation to MII (56% vs. 35% for control) of immature oocytes. Activin A significantly suppressed basal, and inhibin A-and FSH- stimulated progesterone and E2 secretion by nonluteinized granulosa cells. Conclusion(s): Activin A is a promoter of oocyte maturation in vitro and a modulator of granulosa cell steroidogenesis in culture.

Original languageEnglish (US)
Pages (from-to)1126-1130
Number of pages5
JournalFertility and Sterility
Volume70
Issue number6
DOIs
StatePublished - Dec 1998
Externally publishedYes

Fingerprint

Granulosa Cells
Oocytes
Metaphase
Progesterone
Steroids
In Vitro Oocyte Maturation Techniques
Ovariectomy
Outcome Assessment (Health Care)
In Vitro Techniques
activin A
Pathology

Keywords

  • Activin
  • Estradiol
  • Granulosa cells
  • In vitro maturation
  • Inhibin
  • Oocyte
  • Progesterone

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

Activin A stimulates meiotic maturation of human oocytes and modulates granulosa cell steroidogenesis in vitro. / Alak, Baha M.; Coskun, Serdar; Friedman, Chad I.; Kennard, Elizabeth A.; Kim, Moon H.; Seifer, David.

In: Fertility and Sterility, Vol. 70, No. 6, 12.1998, p. 1126-1130.

Research output: Contribution to journalArticle

Alak, Baha M. ; Coskun, Serdar ; Friedman, Chad I. ; Kennard, Elizabeth A. ; Kim, Moon H. ; Seifer, David. / Activin A stimulates meiotic maturation of human oocytes and modulates granulosa cell steroidogenesis in vitro. In: Fertility and Sterility. 1998 ; Vol. 70, No. 6. pp. 1126-1130.
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