Active trans-plasma membrane water cycling in yeast is revealed by NMR

Yajie Zhang, Marie Poirier-Quinot, Charles Jr Springer, James A. Balschi

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Plasma membrane water transport is a crucial cellular phenomenon. Net water movement in response to an osmotic gradient changes cell volume. Steady-state exchange of water molecules, with no net flux or volume change, occurs by passive diffusion through the phospholipid bilayer and passage through membrane proteins. The hypothesis is tested that plasma membrane water exchange also correlates with ATP-driven membrane transport activity in yeast (Saccharomyces cerevisiae). Longitudinal 1H 2O NMR relaxation time constant (T 1) values were measured in yeast suspensions containing extracellular relaxation reagent. Two-site-exchange analysis quantified the reversible exchange kinetics as the mean intracellular water lifetime (τ i), where τ i -1 is the pseudo-first-order rate constant for water efflux. To modulate cellular ATP, yeast suspensions were bubbled with 95%O 2/5%CO 2 (O 2) or 95%N 2/5%CO 2 (N 2). ATP was high during O 2, and τ i -1 was 3.1 s -1 at 25°C. After changing to N 2, ATP decreased and τ i -1 was 1.8 s -1. The principal active yeast ion transport protein is the plasma membrane H +-ATPase. Studies using the H +-ATPase inhibitor ebselen or a yeast genetic strain with reduced H +-ATPase found reduced τ i -1, notwithstanding high ATP. Steady-state water exchange correlates with H +-ATPase activity. At volume steady state, water is cycling across the plasma membrane in response to metabolic transport activity.

Original languageEnglish (US)
Pages (from-to)2833-2842
Number of pages10
JournalBiophysical Journal
Volume101
Issue number11
DOIs
StatePublished - Dec 7 2011

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Yeasts
Cell Membrane
Proton-Translocating ATPases
Water
Adenosine Triphosphate
Carbon Monoxide
Suspensions
Water Movements
Ion Transport
Theophylline
Cell Size
Saccharomyces cerevisiae
Phospholipids
Carrier Proteins
Membrane Proteins
Membranes

ASJC Scopus subject areas

  • Biophysics

Cite this

Active trans-plasma membrane water cycling in yeast is revealed by NMR. / Zhang, Yajie; Poirier-Quinot, Marie; Springer, Charles Jr; Balschi, James A.

In: Biophysical Journal, Vol. 101, No. 11, 07.12.2011, p. 2833-2842.

Research output: Contribution to journalArticle

Zhang, Yajie ; Poirier-Quinot, Marie ; Springer, Charles Jr ; Balschi, James A. / Active trans-plasma membrane water cycling in yeast is revealed by NMR. In: Biophysical Journal. 2011 ; Vol. 101, No. 11. pp. 2833-2842.
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