Activation of muscle-specific genes in pigment, nerve, fat, liver, and fibroblast cell lines by forced expression of MyoD

H. Weintraub, S. J. Tapscott, R. L. Davis, Mathew (Matt) Thayer, M. A. Adam, A. B. Lassar, A. D. Miller

Research output: Contribution to journalArticle

664 Citations (Scopus)

Abstract

MyoD is a master regulatory gene for myogenesis. Under the control of a retroviral long terminal repeat, MyoD was expressed in a variety of differentiated cell types by using either a DNA transfection vector or a retrovirus. Expression of muscle-specific proteins was observed in chicken, human, and rat primary fibroblasts and in differentiated melanoma, neuroblastoma, liver, and adipocyte lines. The ability of MyoD to activate muscle genes in a variety of differentiated cell lines suggests that no additional tissue-specific factors other than MyoD are needed to activate the downstream program for terminal muscle differentiation or that, if such factors exist, they are themselves activated by MyoD expression.

Original languageEnglish (US)
Pages (from-to)5434-5438
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume86
Issue number14
StatePublished - 1989
Externally publishedYes

Fingerprint

Fibroblasts
Fats
Cell Line
Muscles
Terminal Repeat Sequences
Muscle Development
Muscle Proteins
Liver
Thromboplastin
Retroviridae
Regulator Genes
Neuroblastoma
Adipocytes
Genes
Transfection
Chickens
Melanoma
DNA

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Activation of muscle-specific genes in pigment, nerve, fat, liver, and fibroblast cell lines by forced expression of MyoD. / Weintraub, H.; Tapscott, S. J.; Davis, R. L.; Thayer, Mathew (Matt); Adam, M. A.; Lassar, A. B.; Miller, A. D.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 86, No. 14, 1989, p. 5434-5438.

Research output: Contribution to journalArticle

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AU - Lassar, A. B.

AU - Miller, A. D.

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