TY - JOUR
T1 - Activation of membrane-permeant caged PtdIns(3)P induces endosomal fusion in cells
AU - Subramanian, Devaraj
AU - Laketa, Vibor
AU - Müller, Rainer
AU - Tischer, Christian
AU - Zarbakhsh, Sirus
AU - Pepperkok, Rainer
AU - Schultz, Carsten
N1 - Funding Information:
We thank the Advanced Light Microscopy Facility of the European Molecular Biology Laboratory (EMBL), and we also thank C. Funaya and C. Antony of the EMBL Electron Microscopy Core Facility for EM sample preparation. Funding was provided by the Volkswagen Foundation (I81/597 and I81/797) and the Helmholtz-Initiative on Systems Biology (SBCancer) to C.S.
PY - 2010/5
Y1 - 2010/5
N2 - Phosphatidylinositol 3-phosphate (PtdIns(3)P) is a phospholipid residing on early endosomes, where it is proposed to be involved in endosomal fusion. We synthesized membrane-permeant derivatives of PtdIns(3)P, including a caged version that is to our knowledge the first photoactivatable phosphoinositide derivative developed so far. In living cells, photoactivation of caged PtdIns(3)P induced rapid endosomal fusion in an EEA1-dependent fashion, thus providing in vivo evidence that PtdIns(3)P is a sufficient signal for driving this process.
AB - Phosphatidylinositol 3-phosphate (PtdIns(3)P) is a phospholipid residing on early endosomes, where it is proposed to be involved in endosomal fusion. We synthesized membrane-permeant derivatives of PtdIns(3)P, including a caged version that is to our knowledge the first photoactivatable phosphoinositide derivative developed so far. In living cells, photoactivation of caged PtdIns(3)P induced rapid endosomal fusion in an EEA1-dependent fashion, thus providing in vivo evidence that PtdIns(3)P is a sufficient signal for driving this process.
UR - http://www.scopus.com/inward/record.url?scp=77951296416&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77951296416&partnerID=8YFLogxK
U2 - 10.1038/nchembio.348
DO - 10.1038/nchembio.348
M3 - Article
C2 - 20364126
AN - SCOPUS:77951296416
SN - 1552-4450
VL - 6
SP - 324
EP - 326
JO - Nature Chemical Biology
JF - Nature Chemical Biology
IS - 5
ER -