Activation of membrane-permeant caged PtdIns(3)P induces endosomal fusion in cells

Devaraj Subramanian, Vibor Laketa, Rainer Müller, Christian Tischer, Sirus Zarbakhsh, Rainer Pepperkok, Carsten Schultz

Research output: Contribution to journalArticlepeer-review

49 Scopus citations

Abstract

Phosphatidylinositol 3-phosphate (PtdIns(3)P) is a phospholipid residing on early endosomes, where it is proposed to be involved in endosomal fusion. We synthesized membrane-permeant derivatives of PtdIns(3)P, including a caged version that is to our knowledge the first photoactivatable phosphoinositide derivative developed so far. In living cells, photoactivation of caged PtdIns(3)P induced rapid endosomal fusion in an EEA1-dependent fashion, thus providing in vivo evidence that PtdIns(3)P is a sufficient signal for driving this process.

Original languageEnglish (US)
Pages (from-to)324-326
Number of pages3
JournalNature Chemical Biology
Volume6
Issue number5
DOIs
StatePublished - May 2010

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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