Activation of human mononuclear cells by porcine biologic meshes in vitro

Sean Orenstein, Y. Qiao, U. Klueh, D. L. Kreutzer, Y. W. Novitsky

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Introduction While porcine-based biologic meshes are increasingly used for hernia repair, little data exist on tissue responses to such products. Host foreign body reaction, local inXammation, and wound healing are principally controlled by monocytes/macrophages (M/MØs). Exaggerated activation of M/MØs may deleteriously inXuence mesh integration and remodeling. We hypothesized that common porcine meshes induce the differential activation of M/MØs in vitro. Materials and methods Samples of four acellular porcinederived meshes, CollaMend™ (CM; C.R. Bard/Davol), Permacol™ (PC; TSL/Covidien), Strattice™ (ST; Life- Cell), and Surgisis® (SS; Cook Biotech), were exposed to mononuclear cells derived from the peripheral blood of six healthy subjects. Following a 7-day incubation period, supernatants were assayed for interleukin-1beta (IL-1β), interleukin-6 (IL-6), interleukin-8 (IL-8), and vascular endothelial growth factor (VEGF) using a multiplex bead-based immunoassay system. The four groups were compared using analysis of variance (ANOVA) and Student's t-test. Results Each mesh type induced differential mononuclear cell activation in vitro. The mean IL-1β expressions for CM (7,195 pg/ml) and PC (4,215 pg/ml) were signiWcantly higher compared to ST and SS (123 and 998 pg/ml, respectively; P <0.05). Similar trends were also seen for IL-6 (range 445-70,729 pg/ml), IL-8 (range 11,640-1,045,938 pg/ml), and VEGF (range 686-7,133 pg/ml). Conclusion For the Wrst time, we demonstrated that porcine meshes induce M/MØ activation in vitro. CM and PC (chemically crosslinked dermis) induced signiWcantly higher cytokine expression compared to ST (non-crosslinked dermis) and SS (small intestine submucosa). These differences are likely related to proprietary processing methods and/or the extent of collagen crosslinking. Further understanding of immunologic eVects of porcine-derived biologic meshes will not only allow for a comparison between existing products, but it may also lead to mesh modiWcations and improvement of their clinical performance.

Original languageEnglish (US)
Pages (from-to)401-407
Number of pages7
JournalHernia
Volume14
Issue number4
DOIs
StatePublished - Aug 2010
Externally publishedYes

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Swine
Small Intestine
Monocytes
Macrophage Activation
Dermis
Interleukin-8
Interleukin-1beta
Vascular Endothelial Growth Factor A
Interleukin-6
Foreign-Body Reaction
Herniorrhaphy
Immunoassay
Wound Healing
Analysis of Variance
Healthy Volunteers
Collagen
Macrophages
Cytokines
Students
In Vitro Techniques

Keywords

  • Biologic mesh
  • Cytokine
  • Dermis
  • In vitr
  • Interleukin-1beta (IL-1βterleukin-6 (IL-6)
  • Interleukin-8 (IL-8)
  • Monocytes
  • Peripheral blood mononuclear cells (PBMCs)
  • Porcine
  • Small intestine submucosa
  • Vascular endothelial cell growth factor (VEGF)

ASJC Scopus subject areas

  • Surgery

Cite this

Activation of human mononuclear cells by porcine biologic meshes in vitro. / Orenstein, Sean; Qiao, Y.; Klueh, U.; Kreutzer, D. L.; Novitsky, Y. W.

In: Hernia, Vol. 14, No. 4, 08.2010, p. 401-407.

Research output: Contribution to journalArticle

Orenstein, S, Qiao, Y, Klueh, U, Kreutzer, DL & Novitsky, YW 2010, 'Activation of human mononuclear cells by porcine biologic meshes in vitro', Hernia, vol. 14, no. 4, pp. 401-407. https://doi.org/10.1007/s10029-010-0634-7
Orenstein, Sean ; Qiao, Y. ; Klueh, U. ; Kreutzer, D. L. ; Novitsky, Y. W. / Activation of human mononuclear cells by porcine biologic meshes in vitro. In: Hernia. 2010 ; Vol. 14, No. 4. pp. 401-407.
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abstract = "Introduction While porcine-based biologic meshes are increasingly used for hernia repair, little data exist on tissue responses to such products. Host foreign body reaction, local inXammation, and wound healing are principally controlled by monocytes/macrophages (M/M{\O}s). Exaggerated activation of M/M{\O}s may deleteriously inXuence mesh integration and remodeling. We hypothesized that common porcine meshes induce the differential activation of M/M{\O}s in vitro. Materials and methods Samples of four acellular porcinederived meshes, CollaMend™ (CM; C.R. Bard/Davol), Permacol™ (PC; TSL/Covidien), Strattice™ (ST; Life- Cell), and Surgisis{\circledR} (SS; Cook Biotech), were exposed to mononuclear cells derived from the peripheral blood of six healthy subjects. Following a 7-day incubation period, supernatants were assayed for interleukin-1beta (IL-1β), interleukin-6 (IL-6), interleukin-8 (IL-8), and vascular endothelial growth factor (VEGF) using a multiplex bead-based immunoassay system. The four groups were compared using analysis of variance (ANOVA) and Student's t-test. Results Each mesh type induced differential mononuclear cell activation in vitro. The mean IL-1β expressions for CM (7,195 pg/ml) and PC (4,215 pg/ml) were signiWcantly higher compared to ST and SS (123 and 998 pg/ml, respectively; P <0.05). Similar trends were also seen for IL-6 (range 445-70,729 pg/ml), IL-8 (range 11,640-1,045,938 pg/ml), and VEGF (range 686-7,133 pg/ml). Conclusion For the Wrst time, we demonstrated that porcine meshes induce M/M{\O} activation in vitro. CM and PC (chemically crosslinked dermis) induced signiWcantly higher cytokine expression compared to ST (non-crosslinked dermis) and SS (small intestine submucosa). These differences are likely related to proprietary processing methods and/or the extent of collagen crosslinking. Further understanding of immunologic eVects of porcine-derived biologic meshes will not only allow for a comparison between existing products, but it may also lead to mesh modiWcations and improvement of their clinical performance.",
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T1 - Activation of human mononuclear cells by porcine biologic meshes in vitro

AU - Orenstein, Sean

AU - Qiao, Y.

AU - Klueh, U.

AU - Kreutzer, D. L.

AU - Novitsky, Y. W.

PY - 2010/8

Y1 - 2010/8

N2 - Introduction While porcine-based biologic meshes are increasingly used for hernia repair, little data exist on tissue responses to such products. Host foreign body reaction, local inXammation, and wound healing are principally controlled by monocytes/macrophages (M/MØs). Exaggerated activation of M/MØs may deleteriously inXuence mesh integration and remodeling. We hypothesized that common porcine meshes induce the differential activation of M/MØs in vitro. Materials and methods Samples of four acellular porcinederived meshes, CollaMend™ (CM; C.R. Bard/Davol), Permacol™ (PC; TSL/Covidien), Strattice™ (ST; Life- Cell), and Surgisis® (SS; Cook Biotech), were exposed to mononuclear cells derived from the peripheral blood of six healthy subjects. Following a 7-day incubation period, supernatants were assayed for interleukin-1beta (IL-1β), interleukin-6 (IL-6), interleukin-8 (IL-8), and vascular endothelial growth factor (VEGF) using a multiplex bead-based immunoassay system. The four groups were compared using analysis of variance (ANOVA) and Student's t-test. Results Each mesh type induced differential mononuclear cell activation in vitro. The mean IL-1β expressions for CM (7,195 pg/ml) and PC (4,215 pg/ml) were signiWcantly higher compared to ST and SS (123 and 998 pg/ml, respectively; P <0.05). Similar trends were also seen for IL-6 (range 445-70,729 pg/ml), IL-8 (range 11,640-1,045,938 pg/ml), and VEGF (range 686-7,133 pg/ml). Conclusion For the Wrst time, we demonstrated that porcine meshes induce M/MØ activation in vitro. CM and PC (chemically crosslinked dermis) induced signiWcantly higher cytokine expression compared to ST (non-crosslinked dermis) and SS (small intestine submucosa). These differences are likely related to proprietary processing methods and/or the extent of collagen crosslinking. Further understanding of immunologic eVects of porcine-derived biologic meshes will not only allow for a comparison between existing products, but it may also lead to mesh modiWcations and improvement of their clinical performance.

AB - Introduction While porcine-based biologic meshes are increasingly used for hernia repair, little data exist on tissue responses to such products. Host foreign body reaction, local inXammation, and wound healing are principally controlled by monocytes/macrophages (M/MØs). Exaggerated activation of M/MØs may deleteriously inXuence mesh integration and remodeling. We hypothesized that common porcine meshes induce the differential activation of M/MØs in vitro. Materials and methods Samples of four acellular porcinederived meshes, CollaMend™ (CM; C.R. Bard/Davol), Permacol™ (PC; TSL/Covidien), Strattice™ (ST; Life- Cell), and Surgisis® (SS; Cook Biotech), were exposed to mononuclear cells derived from the peripheral blood of six healthy subjects. Following a 7-day incubation period, supernatants were assayed for interleukin-1beta (IL-1β), interleukin-6 (IL-6), interleukin-8 (IL-8), and vascular endothelial growth factor (VEGF) using a multiplex bead-based immunoassay system. The four groups were compared using analysis of variance (ANOVA) and Student's t-test. Results Each mesh type induced differential mononuclear cell activation in vitro. The mean IL-1β expressions for CM (7,195 pg/ml) and PC (4,215 pg/ml) were signiWcantly higher compared to ST and SS (123 and 998 pg/ml, respectively; P <0.05). Similar trends were also seen for IL-6 (range 445-70,729 pg/ml), IL-8 (range 11,640-1,045,938 pg/ml), and VEGF (range 686-7,133 pg/ml). Conclusion For the Wrst time, we demonstrated that porcine meshes induce M/MØ activation in vitro. CM and PC (chemically crosslinked dermis) induced signiWcantly higher cytokine expression compared to ST (non-crosslinked dermis) and SS (small intestine submucosa). These differences are likely related to proprietary processing methods and/or the extent of collagen crosslinking. Further understanding of immunologic eVects of porcine-derived biologic meshes will not only allow for a comparison between existing products, but it may also lead to mesh modiWcations and improvement of their clinical performance.

KW - Biologic mesh

KW - Cytokine

KW - Dermis

KW - In vitr

KW - Interleukin-1beta (IL-1βterleukin-6 (IL-6)

KW - Interleukin-8 (IL-8)

KW - Monocytes

KW - Peripheral blood mononuclear cells (PBMCs)

KW - Porcine

KW - Small intestine submucosa

KW - Vascular endothelial cell growth factor (VEGF)

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