TY - JOUR
T1 - Activation of Ca2+/calmodulin-dependent protein kinase II in cerebellar granule cells by N-methyl-d-aspartate receptor activation
AU - Fukunaga, Koji
AU - Soderling, Thomas R.
PY - 1990/10
Y1 - 1990/10
N2 - Cultured cerebellar granule cells were studied to determine if the excitatory neurotransmitter glutamate acting through the N-methyl-d-aspartate (NMDA) receptor could stimulate autophosphorylation of Ca2+/calmodulin-dependent protein kinase II (CaM-kinase II) to generate its Ca2+-independent form. Glutamate did elevate Ca2+-independent CaM-kinase II through autophosphorylation when granule cells were incubated in Mg2+-free buffer, and this response was potentiated by 1 μM glycine. Extracellular Ca2+ was required, and specific antagonists of the NMDA receptor blocked the response. These results support the hypothesis that postsynaptic Ca2+ influx through the NMDA receptor-gated ion channel, as occurs during induction of long-term potentiation, may convert CaM-kinase II to a constitutively active, Ca2+-independent form.
AB - Cultured cerebellar granule cells were studied to determine if the excitatory neurotransmitter glutamate acting through the N-methyl-d-aspartate (NMDA) receptor could stimulate autophosphorylation of Ca2+/calmodulin-dependent protein kinase II (CaM-kinase II) to generate its Ca2+-independent form. Glutamate did elevate Ca2+-independent CaM-kinase II through autophosphorylation when granule cells were incubated in Mg2+-free buffer, and this response was potentiated by 1 μM glycine. Extracellular Ca2+ was required, and specific antagonists of the NMDA receptor blocked the response. These results support the hypothesis that postsynaptic Ca2+ influx through the NMDA receptor-gated ion channel, as occurs during induction of long-term potentiation, may convert CaM-kinase II to a constitutively active, Ca2+-independent form.
UR - http://www.scopus.com/inward/record.url?scp=0003211823&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0003211823&partnerID=8YFLogxK
U2 - 10.1016/1044-7431(90)90017-X
DO - 10.1016/1044-7431(90)90017-X
M3 - Article
AN - SCOPUS:0003211823
SN - 1044-7431
VL - 1
SP - 133
EP - 138
JO - Molecular and Cellular Neuroscience
JF - Molecular and Cellular Neuroscience
IS - 2
ER -