Activation of A-type γ-aminobutyric acid receptors excites gonadotropin-releasing hormone neurons

R. Anthony DeFazio, Sabine Heger, Sergio Ojeda, Suzanne M. Moenter

    Research output: Contribution to journalArticle

    208 Citations (Scopus)

    Abstract

    γ,-Aminobutyric acid (GABA), acting through GABAA receptors (GABAAR), is hypothesized to suppress reproduction by inhibiting GnRH secretion, but GABA actions directly on GnRH neurons are not well established. In green fluorescent protein-identified adult mouse GnRH neurons in brain slices, gramicidin-perforated-patch-clamp experiments revealed the reversal potential (EGABA) for current through GABAARs was depolarized relative to the resting potential. Furthermore, rapid GABA application elicited action potentials in GnRH neurons but not controls. The consequence of GABAAR activation depends on intracellular chloride levels, which are maintained by homeostatic mechanisms. Membrane proteins that typically extrude chloride (KCC-2 cotransporter, CLC-2 channel) were absent from the GT1-7 immortalized GnRH cell line and GnRH neurons in situ or were not localized to the proper cell compartment for function. In contrast, GT1-7 cells and some GnRH neurons expressed the chloride-accumulating cotransporter, NKCC-1. Patch-clamp experiments showed that blockade of NKCC hyperpolarized EGABA by lowering intracellular chloride. Regardless of reproductive state, rapid GABA application excited GnRH neurons. In contrast, bath application of the GABAAR agonist muscimol transiently increased then suppressed firing; suppression persisted 4-15 min. Rapid activation of GABAAR thus excites GnRH neurons whereas prolonged activation reduces excitability, suggesting the physiological consequence of synaptic activation of GABAAR in GnRH neurons is excitation.

    Original languageEnglish (US)
    Pages (from-to)2872-2891
    Number of pages20
    JournalMolecular Endocrinology
    Volume16
    Issue number12
    DOIs
    StatePublished - Dec 1 2002

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    LHRH Receptors
    Aminobutyrates
    Gonadotropin-Releasing Hormone
    Neurons
    GABA-A Receptors
    gamma-Aminobutyric Acid
    Chlorides
    GABA-A Receptor Agonists
    Gramicidin
    Muscimol
    Green Fluorescent Proteins
    Baths
    Membrane Potentials
    Action Potentials
    Reproduction
    Membrane Proteins

    ASJC Scopus subject areas

    • Molecular Biology
    • Endocrinology, Diabetes and Metabolism

    Cite this

    Activation of A-type γ-aminobutyric acid receptors excites gonadotropin-releasing hormone neurons. / DeFazio, R. Anthony; Heger, Sabine; Ojeda, Sergio; Moenter, Suzanne M.

    In: Molecular Endocrinology, Vol. 16, No. 12, 01.12.2002, p. 2872-2891.

    Research output: Contribution to journalArticle

    DeFazio, R. Anthony ; Heger, Sabine ; Ojeda, Sergio ; Moenter, Suzanne M. / Activation of A-type γ-aminobutyric acid receptors excites gonadotropin-releasing hormone neurons. In: Molecular Endocrinology. 2002 ; Vol. 16, No. 12. pp. 2872-2891.
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