TY - JOUR
T1 - Activation and repression of myogenesis in somatic cell hybrids
T2 - Evidence for trans-negative regulation of MyoD in primary fibroblasts
AU - Thayer, Mathew J.
AU - Welntraub, Harold
N1 - Funding Information:
We thank K. Fournier for providing advice as well as hybrid and primary culture cell lines and human chromosome-specific DNA probes, P Jones for providing the RD cell line, H. Arnold for the Myf-3 cDNA clone, B. Storb, D. Banker, R Whyte, and E. Epner for human chromosome-specific DNA probes, and our colleagues in the Department of Genetics and in the Department of Molecular Medicine for comments during the course of this work and on the manuscript. This work was supported in part by fellowship DRG-1000 of the Damon Runyon-Walter Winchell Cancer Fund (to M. J. T.). This work was supported by a grant to H. W. from the National Institutes of Health.
PY - 1990/10/5
Y1 - 1990/10/5
N2 - We show that transfer of human fibroblast chromosome 11 (containing the human MyoD gene) from primary cells into 10T1 2 mouse fibroblasts by microcell fusion activates expression of the transferred human MyoD gene and converts these cells to myoblasts. Transfer of human chromosome 11 into B78 melanoma cells also leads to the activation of human MyoD. In contrast to the results where a single chromosome 11 is transferred, whole-cell hybrids between 10T1 2 cells and human skin fibroblasts do not express the myogenic phenotype; however, when specific human chromosomes are lost, myogenesis occurs. These results suggest that the MyoD locus is potentially functional in primary human fibroblasts, but is normally repressed in trans by a locus on a different human fibroblast chromosome.
AB - We show that transfer of human fibroblast chromosome 11 (containing the human MyoD gene) from primary cells into 10T1 2 mouse fibroblasts by microcell fusion activates expression of the transferred human MyoD gene and converts these cells to myoblasts. Transfer of human chromosome 11 into B78 melanoma cells also leads to the activation of human MyoD. In contrast to the results where a single chromosome 11 is transferred, whole-cell hybrids between 10T1 2 cells and human skin fibroblasts do not express the myogenic phenotype; however, when specific human chromosomes are lost, myogenesis occurs. These results suggest that the MyoD locus is potentially functional in primary human fibroblasts, but is normally repressed in trans by a locus on a different human fibroblast chromosome.
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U2 - 10.1016/0092-8674(90)90285-M
DO - 10.1016/0092-8674(90)90285-M
M3 - Article
C2 - 2208280
AN - SCOPUS:0025037499
SN - 0092-8674
VL - 63
SP - 23
EP - 32
JO - Cell
JF - Cell
IS - 1
ER -