Activated Src protein tyrosine kinase is overexpressed in late-stage human ovarian cancers

Jon R. Wiener, Christopher Windham, Veronica C. Estrella, Nila U. Parikh, Peter F. Thall, Michael T. Deavers, Robert C. Bast, Gordon B. Mills, Gary E. Gallick

Research output: Contribution to journalArticlepeer-review

156 Scopus citations

Abstract

Objective. The objective of this study was to determine if the Src tyrosine kinase is overexpressed and activated in late-stage human ovarian cancers. Methods. Western analysis and immune complex kinase assays were performed on a panel of human ovarian cancer cell lines and normal ovarian epithelial cell cultures, and immunohistochemical analysis for Src and activated Src were performed on a panel of late-stage human ovarian tumors. Results and conclusions. Src is overexpressed and activated in a majority of late-stage ovarian tumors as well as in a panel of cultured malignant human ovarian epithelium grown in vitro, but not in normal ovarian epithelium (NOE) or immortalized NOE. Src overexpression was found to be frequently, but not always, associated with HER-2/neu overexpression, but no statistical association between Src and Her-2/neu overexpression could be demonstrated.

Original languageEnglish (US)
Pages (from-to)73-79
Number of pages7
JournalGynecologic oncology
Volume88
Issue number1
DOIs
StatePublished - Jan 1 2003
Externally publishedYes

Keywords

  • Immuno-histochemistry
  • Monoclonal antibody
  • Ovarian cancer
  • Src
  • Tyrosine kinase
  • Yes

ASJC Scopus subject areas

  • Oncology
  • Obstetrics and Gynecology

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