Actinomycin D induced DNAse I cleavage enhancement caused by sequence specific propagation of an altered DNA structure

Yao Qi Huang, Robert P. Rehfuss, Steven R. Laplante, Eilis Boudreau, Philip N. Borer, Michael J. Lane

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Two DNA hexadecamers containing one central 5′-GC-3′ base step have been examined by footprinting methodology in the presence and absence of actinomycin D. The results of these studies, coupled with imino proton NMR measurements indicate that the antitumor drug causes a change in DNA conformation at a distance from the actinomycin intercalation site in a molecule of sequence d[ATATATAGCTATATAT] that does not occur in d[AAAAAAAGCTTTTTTT] The experiments demonstrate that DNase I rate enhancements associated with actinomycin D binding are caused by ligand alteration of equilibrium DNA structure.

Original languageEnglish (US)
Pages (from-to)11125-11139
Number of pages15
JournalNucleic Acids Research
Volume16
Issue number23
DOIs
StatePublished - Dec 9 1988
Externally publishedYes

Fingerprint

Dactinomycin
Conformation
Drugs
DNA
Enhancement
Molecules
Propagation
Methodology
Demonstrate
Experiment
Nucleic Acid Conformation
Deoxyribonuclease I
Intercalation
Antineoplastic Agents
Conformations
Protons
Ligands
Nuclear magnetic resonance
Experiments

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)
  • Genetics

Cite this

Actinomycin D induced DNAse I cleavage enhancement caused by sequence specific propagation of an altered DNA structure. / Huang, Yao Qi; Rehfuss, Robert P.; Laplante, Steven R.; Boudreau, Eilis; Borer, Philip N.; Lane, Michael J.

In: Nucleic Acids Research, Vol. 16, No. 23, 09.12.1988, p. 11125-11139.

Research output: Contribution to journalArticle

Huang, Yao Qi ; Rehfuss, Robert P. ; Laplante, Steven R. ; Boudreau, Eilis ; Borer, Philip N. ; Lane, Michael J. / Actinomycin D induced DNAse I cleavage enhancement caused by sequence specific propagation of an altered DNA structure. In: Nucleic Acids Research. 1988 ; Vol. 16, No. 23. pp. 11125-11139.
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