Actin crystal dynamics: Structural implications for F-actin nucleation, polymerization, and branching mediated by the anti-parallel dimer

Robbie Reutzel, Craig Yoshioka, Lakshmanan Govindasamy, Elena G. Yarmola, Mavis Agbandje-McKenna, Michael R. Bubb, Robert McKenna

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Actin filament nucleation, polymerization, and branching are crucial steps in many forms of cell motility, cell shape, and intracellular organelle movements in a wide range of organisms. Previous biochemical data suggests that an anti-parallel actin dimer can incorporate itself into growing filamentous actin (F-actin) and has a role in branching. Furthermore, it is a widespread belief that nucleation is spawned from an actin trimer complex. Here we present the structures of actin dimers and trimers in two tetragonal crystal systems P43212 and P43. Both crystal systems formed by an induced condensation transformation of a previously reported orthorhombic crystal system P212121. Comparison between the three crystal systems demonstrates the dynamics and flexibility of actin-actin interactions. The dimer and trimer actin rearrangements observed between the three crystal systems may provide insight to in vivo actin-actin interactions that occur during the nucleation, polymerization, and branching of F-actin.

Original languageEnglish (US)
Pages (from-to)291-301
Number of pages11
JournalJournal of Structural Biology
Volume146
Issue number3
DOIs
StatePublished - Jun 2004

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Polymerization
Actins
Cell Shape
Actin Cytoskeleton
Organelles
Cell Movement

Keywords

  • Actin
  • Branching
  • Dynamics
  • Nucleation
  • Polymerization

ASJC Scopus subject areas

  • Structural Biology

Cite this

Actin crystal dynamics : Structural implications for F-actin nucleation, polymerization, and branching mediated by the anti-parallel dimer. / Reutzel, Robbie; Yoshioka, Craig; Govindasamy, Lakshmanan; Yarmola, Elena G.; Agbandje-McKenna, Mavis; Bubb, Michael R.; McKenna, Robert.

In: Journal of Structural Biology, Vol. 146, No. 3, 06.2004, p. 291-301.

Research output: Contribution to journalArticle

Reutzel, Robbie ; Yoshioka, Craig ; Govindasamy, Lakshmanan ; Yarmola, Elena G. ; Agbandje-McKenna, Mavis ; Bubb, Michael R. ; McKenna, Robert. / Actin crystal dynamics : Structural implications for F-actin nucleation, polymerization, and branching mediated by the anti-parallel dimer. In: Journal of Structural Biology. 2004 ; Vol. 146, No. 3. pp. 291-301.
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