TY - JOUR
T1 - Actin crystal dynamics
T2 - Structural implications for F-actin nucleation, polymerization, and branching mediated by the anti-parallel dimer
AU - Reutzel, Robbie
AU - Yoshioka, Craig
AU - Govindasamy, Lakshmanan
AU - Yarmola, Elena G.
AU - Agbandje-McKenna, Mavis
AU - Bubb, Michael R.
AU - McKenna, Robert
N1 - Funding Information:
This work was supported by the Office of Research and Development, Medical Research Service of the Department of Veterans Affairs (M.R.B.) and a College Incentive Fund from the College of Medicine, University of Florida (R.M. and M.R.B.). The authors also thank Nathan Bryant for his help in making the figures that appear in the manuscript.
PY - 2004/6
Y1 - 2004/6
N2 - Actin filament nucleation, polymerization, and branching are crucial steps in many forms of cell motility, cell shape, and intracellular organelle movements in a wide range of organisms. Previous biochemical data suggests that an anti-parallel actin dimer can incorporate itself into growing filamentous actin (F-actin) and has a role in branching. Furthermore, it is a widespread belief that nucleation is spawned from an actin trimer complex. Here we present the structures of actin dimers and trimers in two tetragonal crystal systems P43212 and P43. Both crystal systems formed by an induced condensation transformation of a previously reported orthorhombic crystal system P212121. Comparison between the three crystal systems demonstrates the dynamics and flexibility of actin-actin interactions. The dimer and trimer actin rearrangements observed between the three crystal systems may provide insight to in vivo actin-actin interactions that occur during the nucleation, polymerization, and branching of F-actin.
AB - Actin filament nucleation, polymerization, and branching are crucial steps in many forms of cell motility, cell shape, and intracellular organelle movements in a wide range of organisms. Previous biochemical data suggests that an anti-parallel actin dimer can incorporate itself into growing filamentous actin (F-actin) and has a role in branching. Furthermore, it is a widespread belief that nucleation is spawned from an actin trimer complex. Here we present the structures of actin dimers and trimers in two tetragonal crystal systems P43212 and P43. Both crystal systems formed by an induced condensation transformation of a previously reported orthorhombic crystal system P212121. Comparison between the three crystal systems demonstrates the dynamics and flexibility of actin-actin interactions. The dimer and trimer actin rearrangements observed between the three crystal systems may provide insight to in vivo actin-actin interactions that occur during the nucleation, polymerization, and branching of F-actin.
KW - Actin
KW - Branching
KW - Dynamics
KW - Nucleation
KW - Polymerization
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U2 - 10.1016/j.jsb.2003.12.006
DO - 10.1016/j.jsb.2003.12.006
M3 - Article
C2 - 15099571
AN - SCOPUS:1942425451
SN - 1047-8477
VL - 146
SP - 291
EP - 301
JO - Journal of Structural Biology
JF - Journal of Structural Biology
IS - 3
ER -