Acquisition of iron by Trichodesmium and associated bacteria in culture

Kelly L. Roe, Katherine Barbeau, Elizabeth L. Mann, Margo Haygood

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Trichodesmium colonies contain an abundant microbial consortium that is likely to play a role in nutrient cycling within the colony. This study used laboratory cultures of Trichodesmium and two genome-sequenced strains of bacteria typical of Trichodesmium-associated microbes to develop an understanding of the cycling of iron, a potentially limiting micronutrient, within Trichodesmium colonies. We found that the ferric siderophores desferrioxamine B and aerobactin were not readily bioavailable to Trichodesmium, relative to ferric chloride or citrate-associated iron. In contrast, the representative bacterial strains we studied were able to acquire iron from all of the iron sources, implying that naturally occurring Trichodesmium-associated bacteria may be capable of utilizing a more diverse array of iron sources than Trichodesmium. From the organism-specific uptake data collected in this study, a theoretical Trichodesmium colony was designed to model whole colony iron uptake. The bacteria accounted for most (>70%) of the iron acquired by the colony, highlighting the importance of determining organism-specific uptake in a complex environment. Our findings suggest that, although they may share the same micro-environment, Trichodesmium and its colony-associated microbial cohort may differ substantially in terms of iron acquisition strategy.

Original languageEnglish (US)
Pages (from-to)1681-1695
Number of pages15
JournalEnvironmental Microbiology
Volume14
Issue number7
DOIs
StatePublished - Jul 2012

Fingerprint

Trichodesmium
Iron
iron
Bacteria
bacterium
uptake mechanisms
bacteria
Microbial Consortia
deferoxamine
siderophore
microbial culture
Siderophores
Deferoxamine
nutrient cycling
siderophores
Micronutrients
organisms
dietary minerals
citrates
biogeochemical cycles

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Acquisition of iron by Trichodesmium and associated bacteria in culture. / Roe, Kelly L.; Barbeau, Katherine; Mann, Elizabeth L.; Haygood, Margo.

In: Environmental Microbiology, Vol. 14, No. 7, 07.2012, p. 1681-1695.

Research output: Contribution to journalArticle

Roe, Kelly L. ; Barbeau, Katherine ; Mann, Elizabeth L. ; Haygood, Margo. / Acquisition of iron by Trichodesmium and associated bacteria in culture. In: Environmental Microbiology. 2012 ; Vol. 14, No. 7. pp. 1681-1695.
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