Acid/pepsin promotion of carcinogenesis in the hamster cheek pouch

Jeff Adams, Patrick Heintz, Neil Gross, Peter Andersen, Edwin Everts, Mark Wax, James Cohen

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Objective: While clinical observation has suggested an association between gastroesophageal reflux and laryngeal carcinoma, the nature of this relationship has yet to be defined. The purpose of this study is to determine the carcinogenic potential of acid and pepsin mixtures in the hamster cheek pouch animal model. Design: A blinded intervention study. Subjects: One hundred male Syrian hamsters aged approximately 5 weeks. Interventions: A control group of 20 hamsters received application of the carcinogen 9,10- dimethyl-1,2-benzanthracene (DMBA) to their cheek pouch mucosa. One experimental group (n = 20) received applications of DMBA plus hydrochloric acid, and another (n = 20) received DMBA plus an acid and pepsin solution. Latency to squamous cell tumor production, size of tumors, and numbers of tumors were compared among groups. Results: Latency to tumor production and size of tumor were similar among groups, with both experimental and control groups developing tumors of comparable size after 12 weeks of chemical application. However, the number of tumors produced was significantly higher in the DMBA/acid and DMBA/acid/pepsin groups than in the DMBA only group at 18 weeks, with 23, 27, and 10 tumors in these groups, respectively (P

Original languageEnglish (US)
Pages (from-to)405-409
Number of pages5
JournalArchives of Otolaryngology - Head and Neck Surgery
Volume126
Issue number3
StatePublished - Mar 2000

Fingerprint

Cheek
Pepsin A
Cricetinae
Carcinogenesis
Acids
Neoplasms
9,10-Dimethyl-1,2-benzanthracene
Control Groups
Hydrochloric Acid
Mesocricetus
Gastroesophageal Reflux
Carcinogens
Mucous Membrane
Animal Models
Epithelial Cells
Observation
benz(a)anthracene
Carcinoma

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Acid/pepsin promotion of carcinogenesis in the hamster cheek pouch. / Adams, Jeff; Heintz, Patrick; Gross, Neil; Andersen, Peter; Everts, Edwin; Wax, Mark; Cohen, James.

In: Archives of Otolaryngology - Head and Neck Surgery, Vol. 126, No. 3, 03.2000, p. 405-409.

Research output: Contribution to journalArticle

@article{a47c71c10677470f8236bf264e6df08b,
title = "Acid/pepsin promotion of carcinogenesis in the hamster cheek pouch",
abstract = "Objective: While clinical observation has suggested an association between gastroesophageal reflux and laryngeal carcinoma, the nature of this relationship has yet to be defined. The purpose of this study is to determine the carcinogenic potential of acid and pepsin mixtures in the hamster cheek pouch animal model. Design: A blinded intervention study. Subjects: One hundred male Syrian hamsters aged approximately 5 weeks. Interventions: A control group of 20 hamsters received application of the carcinogen 9,10- dimethyl-1,2-benzanthracene (DMBA) to their cheek pouch mucosa. One experimental group (n = 20) received applications of DMBA plus hydrochloric acid, and another (n = 20) received DMBA plus an acid and pepsin solution. Latency to squamous cell tumor production, size of tumors, and numbers of tumors were compared among groups. Results: Latency to tumor production and size of tumor were similar among groups, with both experimental and control groups developing tumors of comparable size after 12 weeks of chemical application. However, the number of tumors produced was significantly higher in the DMBA/acid and DMBA/acid/pepsin groups than in the DMBA only group at 18 weeks, with 23, 27, and 10 tumors in these groups, respectively (P",
author = "Jeff Adams and Patrick Heintz and Neil Gross and Peter Andersen and Edwin Everts and Mark Wax and James Cohen",
year = "2000",
month = "3",
language = "English (US)",
volume = "126",
pages = "405--409",
journal = "JAMA Otolaryngology - Head and Neck Surgery",
issn = "2168-6181",
publisher = "American Medical Association",
number = "3",

}

TY - JOUR

T1 - Acid/pepsin promotion of carcinogenesis in the hamster cheek pouch

AU - Adams, Jeff

AU - Heintz, Patrick

AU - Gross, Neil

AU - Andersen, Peter

AU - Everts, Edwin

AU - Wax, Mark

AU - Cohen, James

PY - 2000/3

Y1 - 2000/3

N2 - Objective: While clinical observation has suggested an association between gastroesophageal reflux and laryngeal carcinoma, the nature of this relationship has yet to be defined. The purpose of this study is to determine the carcinogenic potential of acid and pepsin mixtures in the hamster cheek pouch animal model. Design: A blinded intervention study. Subjects: One hundred male Syrian hamsters aged approximately 5 weeks. Interventions: A control group of 20 hamsters received application of the carcinogen 9,10- dimethyl-1,2-benzanthracene (DMBA) to their cheek pouch mucosa. One experimental group (n = 20) received applications of DMBA plus hydrochloric acid, and another (n = 20) received DMBA plus an acid and pepsin solution. Latency to squamous cell tumor production, size of tumors, and numbers of tumors were compared among groups. Results: Latency to tumor production and size of tumor were similar among groups, with both experimental and control groups developing tumors of comparable size after 12 weeks of chemical application. However, the number of tumors produced was significantly higher in the DMBA/acid and DMBA/acid/pepsin groups than in the DMBA only group at 18 weeks, with 23, 27, and 10 tumors in these groups, respectively (P

AB - Objective: While clinical observation has suggested an association between gastroesophageal reflux and laryngeal carcinoma, the nature of this relationship has yet to be defined. The purpose of this study is to determine the carcinogenic potential of acid and pepsin mixtures in the hamster cheek pouch animal model. Design: A blinded intervention study. Subjects: One hundred male Syrian hamsters aged approximately 5 weeks. Interventions: A control group of 20 hamsters received application of the carcinogen 9,10- dimethyl-1,2-benzanthracene (DMBA) to their cheek pouch mucosa. One experimental group (n = 20) received applications of DMBA plus hydrochloric acid, and another (n = 20) received DMBA plus an acid and pepsin solution. Latency to squamous cell tumor production, size of tumors, and numbers of tumors were compared among groups. Results: Latency to tumor production and size of tumor were similar among groups, with both experimental and control groups developing tumors of comparable size after 12 weeks of chemical application. However, the number of tumors produced was significantly higher in the DMBA/acid and DMBA/acid/pepsin groups than in the DMBA only group at 18 weeks, with 23, 27, and 10 tumors in these groups, respectively (P

UR - http://www.scopus.com/inward/record.url?scp=0034014696&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034014696&partnerID=8YFLogxK

M3 - Article

C2 - 10722017

AN - SCOPUS:0034014696

VL - 126

SP - 405

EP - 409

JO - JAMA Otolaryngology - Head and Neck Surgery

JF - JAMA Otolaryngology - Head and Neck Surgery

SN - 2168-6181

IS - 3

ER -