Acid-sensing ion channels in mouse olfactory bulb M/T neurons

Ming Hua Li, Selina Qiuying Liu, Koichi Inoue, Jinquan Lan, Roger P. Simon, Zhi Gang Xiong

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

The olfactory bulb contains the first synaptic relay in the olfactory pathway, the sensory system in which odorants are detected enabling these chemical stimuli to be transformed into electrical signals and, ultimately, the perception of odor. Acid-sensing ion channels (ASICs), a family of proton-gated cation channels, are widely expressed in neurons of the central nervous system. However, no direct electrophysiological and pharmacological characterizations of ASICs in olfactory bulb neurons have been described. Using a combination of whole-cell patch-clamp recordings and biochemical and molecular biological analyses, we demonstrated that functional ASICs exist in mouse olfactory bulb mitral/tufted (M/T) neurons and mainly consist of homomeric ASIC1a and heteromeric ASIC1a/2a channels. ASIC activation depolarized cultured M/T neurons and increased their intracellular calcium concentration. Thus, ASIC activation may play an important role in normal olfactory function.

Original languageEnglish (US)
Pages (from-to)719-731
Number of pages13
JournalJournal of General Physiology
Volume143
Issue number6
DOIs
StatePublished - Jun 2014

ASJC Scopus subject areas

  • Physiology

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    Li, M. H., Liu, S. Q., Inoue, K., Lan, J., Simon, R. P., & Xiong, Z. G. (2014). Acid-sensing ion channels in mouse olfactory bulb M/T neurons. Journal of General Physiology, 143(6), 719-731. https://doi.org/10.1085/jgp.201310990