Acid-sensing ion channels in mouse olfactory bulb M/T neurons

Minghua Li, Selina Qiuying Liu, Koichi Inoue, Jinquan Lan, Roger P. Simon, Zhi Gang Xiong

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The olfactory bulb contains the first synaptic relay in the olfactory pathway, the sensory system in which odorants are detected enabling these chemical stimuli to be transformed into electrical signals and, ultimately, the perception of odor. Acid-sensing ion channels (ASICs), a family of proton-gated cation channels, are widely expressed in neurons of the central nervous system. However, no direct electrophysiological and pharmacological characterizations of ASICs in olfactory bulb neurons have been described. Using a combination of whole-cell patch-clamp recordings and biochemical and molecular biological analyses, we demonstrated that functional ASICs exist in mouse olfactory bulb mitral/tufted (M/T) neurons and mainly consist of homomeric ASIC1a and heteromeric ASIC1a/2a channels. ASIC activation depolarized cultured M/T neurons and increased their intracellular calcium concentration. Thus, ASIC activation may play an important role in normal olfactory function.

Original languageEnglish (US)
Pages (from-to)719-731
Number of pages13
JournalJournal of General Physiology
Volume143
Issue number6
DOIs
StatePublished - 2014

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Acid Sensing Ion Channels
Olfactory Bulb
Neurons
Olfactory Pathways
Cations
Protons
Central Nervous System
Pharmacology
Calcium

ASJC Scopus subject areas

  • Physiology

Cite this

Li, M., Liu, S. Q., Inoue, K., Lan, J., Simon, R. P., & Xiong, Z. G. (2014). Acid-sensing ion channels in mouse olfactory bulb M/T neurons. Journal of General Physiology, 143(6), 719-731. https://doi.org/10.1085/jgp.201310990

Acid-sensing ion channels in mouse olfactory bulb M/T neurons. / Li, Minghua; Liu, Selina Qiuying; Inoue, Koichi; Lan, Jinquan; Simon, Roger P.; Xiong, Zhi Gang.

In: Journal of General Physiology, Vol. 143, No. 6, 2014, p. 719-731.

Research output: Contribution to journalArticle

Li, M, Liu, SQ, Inoue, K, Lan, J, Simon, RP & Xiong, ZG 2014, 'Acid-sensing ion channels in mouse olfactory bulb M/T neurons', Journal of General Physiology, vol. 143, no. 6, pp. 719-731. https://doi.org/10.1085/jgp.201310990
Li, Minghua ; Liu, Selina Qiuying ; Inoue, Koichi ; Lan, Jinquan ; Simon, Roger P. ; Xiong, Zhi Gang. / Acid-sensing ion channels in mouse olfactory bulb M/T neurons. In: Journal of General Physiology. 2014 ; Vol. 143, No. 6. pp. 719-731.
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