Acetylcholine is an autocrine or paracrine hormone synthesized and secreted by airway bronchial epithelial cells

Becky J. Proskocil, Harmanjatinder S. Sekhon, Yibing Jia, Valentina Savchenko, Randy D. Blakely, Jon Lindstrom, Eliot Spindel

    Research output: Contribution to journalArticle

    156 Citations (Scopus)

    Abstract

    The role of acetylcholine (ACh) as a key neurotransmitter in the central and peripheral nervous system is well established. However, the role of ACh may be broader because ACh may also function as an autocrine or paracrine signaling molecule in a variety of nonneuronal tissues. To begin to establish ACh of nonneuronal origin as a paracrine hormone in lung, we have examined neonatal and adult monkey bronchial epithelium for the components involved in nicotinic cholinergic signaling. Using immunohistochemistry and RT-PCR, we have demonstrated in lung bronchial epithelial cells (BECs) expression of choline acetyltransferase, the vesicular ACh transporter, the choline high-affinity transporter, α7, α4, and β2 nicotinic ACh receptor (nAChR) subunits, and the nAChR accessory protein lynxl. Confocal microscopy demonstrates that these factors are expressed in epithelial cells and are clearly distinct from neighboring nerve fibers. Confirmation of RNA identity has been confirmed by partial sequence analysis of PCR products and by cDNA cloning. Primary culture of BECs confirms the synthesis and secretion of ACh and the activity of cholinesterases. Thus, ACh meets all the criteria for an autocrine/paracrine hormone in lung bronchial epithelium. The nonneuronal cholinergic signaling pathway in lung provides a potentially important target for cholinergic drugs. This pathway may also explain some of the effects of nicotine on fetal development and also provides additional mechanisms by which smoking affects lung cancer growth and development.

    Original languageEnglish (US)
    Pages (from-to)2498-2506
    Number of pages9
    JournalEndocrinology
    Volume145
    Issue number5
    DOIs
    StatePublished - May 2004

    Fingerprint

    Acetylcholine
    Epithelial Cells
    Hormones
    Cholinergic Agents
    Lung
    Nicotinic Receptors
    Epithelium
    Vesicular Acetylcholine Transport Proteins
    Autocrine Communication
    Paracrine Communication
    Polymerase Chain Reaction
    Choline O-Acetyltransferase
    Cholinesterases
    Peripheral Nervous System
    Cholinergic Receptors
    Fetal Development
    Nicotine
    Growth and Development
    Nerve Fibers
    Confocal Microscopy

    ASJC Scopus subject areas

    • Endocrinology
    • Endocrinology, Diabetes and Metabolism

    Cite this

    Proskocil, B. J., Sekhon, H. S., Jia, Y., Savchenko, V., Blakely, R. D., Lindstrom, J., & Spindel, E. (2004). Acetylcholine is an autocrine or paracrine hormone synthesized and secreted by airway bronchial epithelial cells. Endocrinology, 145(5), 2498-2506. https://doi.org/10.1210/en.2003-1728

    Acetylcholine is an autocrine or paracrine hormone synthesized and secreted by airway bronchial epithelial cells. / Proskocil, Becky J.; Sekhon, Harmanjatinder S.; Jia, Yibing; Savchenko, Valentina; Blakely, Randy D.; Lindstrom, Jon; Spindel, Eliot.

    In: Endocrinology, Vol. 145, No. 5, 05.2004, p. 2498-2506.

    Research output: Contribution to journalArticle

    Proskocil, BJ, Sekhon, HS, Jia, Y, Savchenko, V, Blakely, RD, Lindstrom, J & Spindel, E 2004, 'Acetylcholine is an autocrine or paracrine hormone synthesized and secreted by airway bronchial epithelial cells', Endocrinology, vol. 145, no. 5, pp. 2498-2506. https://doi.org/10.1210/en.2003-1728
    Proskocil, Becky J. ; Sekhon, Harmanjatinder S. ; Jia, Yibing ; Savchenko, Valentina ; Blakely, Randy D. ; Lindstrom, Jon ; Spindel, Eliot. / Acetylcholine is an autocrine or paracrine hormone synthesized and secreted by airway bronchial epithelial cells. In: Endocrinology. 2004 ; Vol. 145, No. 5. pp. 2498-2506.
    @article{7df5f67b4f0f4bfab944b1c070bd644f,
    title = "Acetylcholine is an autocrine or paracrine hormone synthesized and secreted by airway bronchial epithelial cells",
    abstract = "The role of acetylcholine (ACh) as a key neurotransmitter in the central and peripheral nervous system is well established. However, the role of ACh may be broader because ACh may also function as an autocrine or paracrine signaling molecule in a variety of nonneuronal tissues. To begin to establish ACh of nonneuronal origin as a paracrine hormone in lung, we have examined neonatal and adult monkey bronchial epithelium for the components involved in nicotinic cholinergic signaling. Using immunohistochemistry and RT-PCR, we have demonstrated in lung bronchial epithelial cells (BECs) expression of choline acetyltransferase, the vesicular ACh transporter, the choline high-affinity transporter, α7, α4, and β2 nicotinic ACh receptor (nAChR) subunits, and the nAChR accessory protein lynxl. Confocal microscopy demonstrates that these factors are expressed in epithelial cells and are clearly distinct from neighboring nerve fibers. Confirmation of RNA identity has been confirmed by partial sequence analysis of PCR products and by cDNA cloning. Primary culture of BECs confirms the synthesis and secretion of ACh and the activity of cholinesterases. Thus, ACh meets all the criteria for an autocrine/paracrine hormone in lung bronchial epithelium. The nonneuronal cholinergic signaling pathway in lung provides a potentially important target for cholinergic drugs. This pathway may also explain some of the effects of nicotine on fetal development and also provides additional mechanisms by which smoking affects lung cancer growth and development.",
    author = "Proskocil, {Becky J.} and Sekhon, {Harmanjatinder S.} and Yibing Jia and Valentina Savchenko and Blakely, {Randy D.} and Jon Lindstrom and Eliot Spindel",
    year = "2004",
    month = "5",
    doi = "10.1210/en.2003-1728",
    language = "English (US)",
    volume = "145",
    pages = "2498--2506",
    journal = "Endocrinology",
    issn = "0013-7227",
    publisher = "The Endocrine Society",
    number = "5",

    }

    TY - JOUR

    T1 - Acetylcholine is an autocrine or paracrine hormone synthesized and secreted by airway bronchial epithelial cells

    AU - Proskocil, Becky J.

    AU - Sekhon, Harmanjatinder S.

    AU - Jia, Yibing

    AU - Savchenko, Valentina

    AU - Blakely, Randy D.

    AU - Lindstrom, Jon

    AU - Spindel, Eliot

    PY - 2004/5

    Y1 - 2004/5

    N2 - The role of acetylcholine (ACh) as a key neurotransmitter in the central and peripheral nervous system is well established. However, the role of ACh may be broader because ACh may also function as an autocrine or paracrine signaling molecule in a variety of nonneuronal tissues. To begin to establish ACh of nonneuronal origin as a paracrine hormone in lung, we have examined neonatal and adult monkey bronchial epithelium for the components involved in nicotinic cholinergic signaling. Using immunohistochemistry and RT-PCR, we have demonstrated in lung bronchial epithelial cells (BECs) expression of choline acetyltransferase, the vesicular ACh transporter, the choline high-affinity transporter, α7, α4, and β2 nicotinic ACh receptor (nAChR) subunits, and the nAChR accessory protein lynxl. Confocal microscopy demonstrates that these factors are expressed in epithelial cells and are clearly distinct from neighboring nerve fibers. Confirmation of RNA identity has been confirmed by partial sequence analysis of PCR products and by cDNA cloning. Primary culture of BECs confirms the synthesis and secretion of ACh and the activity of cholinesterases. Thus, ACh meets all the criteria for an autocrine/paracrine hormone in lung bronchial epithelium. The nonneuronal cholinergic signaling pathway in lung provides a potentially important target for cholinergic drugs. This pathway may also explain some of the effects of nicotine on fetal development and also provides additional mechanisms by which smoking affects lung cancer growth and development.

    AB - The role of acetylcholine (ACh) as a key neurotransmitter in the central and peripheral nervous system is well established. However, the role of ACh may be broader because ACh may also function as an autocrine or paracrine signaling molecule in a variety of nonneuronal tissues. To begin to establish ACh of nonneuronal origin as a paracrine hormone in lung, we have examined neonatal and adult monkey bronchial epithelium for the components involved in nicotinic cholinergic signaling. Using immunohistochemistry and RT-PCR, we have demonstrated in lung bronchial epithelial cells (BECs) expression of choline acetyltransferase, the vesicular ACh transporter, the choline high-affinity transporter, α7, α4, and β2 nicotinic ACh receptor (nAChR) subunits, and the nAChR accessory protein lynxl. Confocal microscopy demonstrates that these factors are expressed in epithelial cells and are clearly distinct from neighboring nerve fibers. Confirmation of RNA identity has been confirmed by partial sequence analysis of PCR products and by cDNA cloning. Primary culture of BECs confirms the synthesis and secretion of ACh and the activity of cholinesterases. Thus, ACh meets all the criteria for an autocrine/paracrine hormone in lung bronchial epithelium. The nonneuronal cholinergic signaling pathway in lung provides a potentially important target for cholinergic drugs. This pathway may also explain some of the effects of nicotine on fetal development and also provides additional mechanisms by which smoking affects lung cancer growth and development.

    UR - http://www.scopus.com/inward/record.url?scp=2042443069&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=2042443069&partnerID=8YFLogxK

    U2 - 10.1210/en.2003-1728

    DO - 10.1210/en.2003-1728

    M3 - Article

    C2 - 14764638

    AN - SCOPUS:2042443069

    VL - 145

    SP - 2498

    EP - 2506

    JO - Endocrinology

    JF - Endocrinology

    SN - 0013-7227

    IS - 5

    ER -