Absorption and chemopreventive targets of sulforaphane in humans following consumption of broccoli sprouts or a myrosinase-treated broccoli sprout extract

Lauren L. Atwell, Anna Hsu, Carmen P. Wong, Jan F. Stevens, Deborah Bella, Tian Wei Yu, Clifford B. Pereira, Christiane V. Löhr, John Mark Christensen, Roderick H. Dashwood, David E. Williams, Jackilen (Jackie) Shannon, Emily Ho

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Scope: Sulforaphane (SFN), an isothiocyanate derived from crucifers, has numerous health benefits. SFN bioavailability from dietary sources is a critical determinant of its efficacy in humans. A key factor in SFN absorption is the release of SFN from its glucosinolate precursor, glucoraphanin, by myrosinase. Dietary supplements are used in clinical trials to deliver consistent SFN doses, but myrosinase is often inactivated in available supplements. We evaluated SFN absorption from a myrosinase-treated broccoli sprout extract (BSE) and are the first to report effects of twice daily, oral dosing on SFN exposure in healthy adults. Methods and results: Subjects consumed fresh broccoli sprouts or the BSE, each providing 200 μmol SFN daily, as a single dose and as two 100-μmol doses taken 12 h apart. Using HPLC-MS/MS, we detected ~3 × higher SFN metabolite levels in plasma and urine of sprout consumers, indicating enhanced SFN absorption from sprouts. Twelve-hour dosing retained higher plasma SFN metabolite levels at later time points than 24-hour dosing. No dose responses were observed for molecular targets of SFN (i.e. heme oxygenase-1, histone deacetylase activity, p21). Conclusion: We conclude that the dietary form and dosing schedule of SFN may impact SFN absorption and efficacy in human trials.

Original languageEnglish (US)
Pages (from-to)424-433
Number of pages10
JournalMolecular Nutrition and Food Research
Volume59
Issue number3
DOIs
StatePublished - Mar 1 2015

Fingerprint

thioglucosidase
sprouts (food)
Brassica
broccoli
extracts
heme oxygenase (biliverdin-producing)
dosage
glucoraphanin
metabolites
histone deacetylase
isothiocyanates
glucosinolates
Brassicaceae
dose response
dietary supplements
bioavailability
clinical trials
mouth
urine
sulforafan

Keywords

  • Absorption
  • Broccoli sprout
  • Chemoprevention
  • Excretion
  • Sulforaphane

ASJC Scopus subject areas

  • Food Science
  • Biotechnology

Cite this

Absorption and chemopreventive targets of sulforaphane in humans following consumption of broccoli sprouts or a myrosinase-treated broccoli sprout extract. / Atwell, Lauren L.; Hsu, Anna; Wong, Carmen P.; Stevens, Jan F.; Bella, Deborah; Yu, Tian Wei; Pereira, Clifford B.; Löhr, Christiane V.; Christensen, John Mark; Dashwood, Roderick H.; Williams, David E.; Shannon, Jackilen (Jackie); Ho, Emily.

In: Molecular Nutrition and Food Research, Vol. 59, No. 3, 01.03.2015, p. 424-433.

Research output: Contribution to journalArticle

Atwell, LL, Hsu, A, Wong, CP, Stevens, JF, Bella, D, Yu, TW, Pereira, CB, Löhr, CV, Christensen, JM, Dashwood, RH, Williams, DE, Shannon, JJ & Ho, E 2015, 'Absorption and chemopreventive targets of sulforaphane in humans following consumption of broccoli sprouts or a myrosinase-treated broccoli sprout extract', Molecular Nutrition and Food Research, vol. 59, no. 3, pp. 424-433. https://doi.org/10.1002/mnfr.201400674
Atwell, Lauren L. ; Hsu, Anna ; Wong, Carmen P. ; Stevens, Jan F. ; Bella, Deborah ; Yu, Tian Wei ; Pereira, Clifford B. ; Löhr, Christiane V. ; Christensen, John Mark ; Dashwood, Roderick H. ; Williams, David E. ; Shannon, Jackilen (Jackie) ; Ho, Emily. / Absorption and chemopreventive targets of sulforaphane in humans following consumption of broccoli sprouts or a myrosinase-treated broccoli sprout extract. In: Molecular Nutrition and Food Research. 2015 ; Vol. 59, No. 3. pp. 424-433.
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abstract = "Scope: Sulforaphane (SFN), an isothiocyanate derived from crucifers, has numerous health benefits. SFN bioavailability from dietary sources is a critical determinant of its efficacy in humans. A key factor in SFN absorption is the release of SFN from its glucosinolate precursor, glucoraphanin, by myrosinase. Dietary supplements are used in clinical trials to deliver consistent SFN doses, but myrosinase is often inactivated in available supplements. We evaluated SFN absorption from a myrosinase-treated broccoli sprout extract (BSE) and are the first to report effects of twice daily, oral dosing on SFN exposure in healthy adults. Methods and results: Subjects consumed fresh broccoli sprouts or the BSE, each providing 200 μmol SFN daily, as a single dose and as two 100-μmol doses taken 12 h apart. Using HPLC-MS/MS, we detected ~3 × higher SFN metabolite levels in plasma and urine of sprout consumers, indicating enhanced SFN absorption from sprouts. Twelve-hour dosing retained higher plasma SFN metabolite levels at later time points than 24-hour dosing. No dose responses were observed for molecular targets of SFN (i.e. heme oxygenase-1, histone deacetylase activity, p21). Conclusion: We conclude that the dietary form and dosing schedule of SFN may impact SFN absorption and efficacy in human trials.",
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AU - Hsu, Anna

AU - Wong, Carmen P.

AU - Stevens, Jan F.

AU - Bella, Deborah

AU - Yu, Tian Wei

AU - Pereira, Clifford B.

AU - Löhr, Christiane V.

AU - Christensen, John Mark

AU - Dashwood, Roderick H.

AU - Williams, David E.

AU - Shannon, Jackilen (Jackie)

AU - Ho, Emily

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N2 - Scope: Sulforaphane (SFN), an isothiocyanate derived from crucifers, has numerous health benefits. SFN bioavailability from dietary sources is a critical determinant of its efficacy in humans. A key factor in SFN absorption is the release of SFN from its glucosinolate precursor, glucoraphanin, by myrosinase. Dietary supplements are used in clinical trials to deliver consistent SFN doses, but myrosinase is often inactivated in available supplements. We evaluated SFN absorption from a myrosinase-treated broccoli sprout extract (BSE) and are the first to report effects of twice daily, oral dosing on SFN exposure in healthy adults. Methods and results: Subjects consumed fresh broccoli sprouts or the BSE, each providing 200 μmol SFN daily, as a single dose and as two 100-μmol doses taken 12 h apart. Using HPLC-MS/MS, we detected ~3 × higher SFN metabolite levels in plasma and urine of sprout consumers, indicating enhanced SFN absorption from sprouts. Twelve-hour dosing retained higher plasma SFN metabolite levels at later time points than 24-hour dosing. No dose responses were observed for molecular targets of SFN (i.e. heme oxygenase-1, histone deacetylase activity, p21). Conclusion: We conclude that the dietary form and dosing schedule of SFN may impact SFN absorption and efficacy in human trials.

AB - Scope: Sulforaphane (SFN), an isothiocyanate derived from crucifers, has numerous health benefits. SFN bioavailability from dietary sources is a critical determinant of its efficacy in humans. A key factor in SFN absorption is the release of SFN from its glucosinolate precursor, glucoraphanin, by myrosinase. Dietary supplements are used in clinical trials to deliver consistent SFN doses, but myrosinase is often inactivated in available supplements. We evaluated SFN absorption from a myrosinase-treated broccoli sprout extract (BSE) and are the first to report effects of twice daily, oral dosing on SFN exposure in healthy adults. Methods and results: Subjects consumed fresh broccoli sprouts or the BSE, each providing 200 μmol SFN daily, as a single dose and as two 100-μmol doses taken 12 h apart. Using HPLC-MS/MS, we detected ~3 × higher SFN metabolite levels in plasma and urine of sprout consumers, indicating enhanced SFN absorption from sprouts. Twelve-hour dosing retained higher plasma SFN metabolite levels at later time points than 24-hour dosing. No dose responses were observed for molecular targets of SFN (i.e. heme oxygenase-1, histone deacetylase activity, p21). Conclusion: We conclude that the dietary form and dosing schedule of SFN may impact SFN absorption and efficacy in human trials.

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