Absence of antibodies to non-NMDA glutamate-receptor subunits in paraneoplastic cerebellar degeneration

A. Degenhardt, Robert Duvoisin, J. Frennier, Sakir Gultekin, M. R. Rosenfeld, J. B. Posner, Josep Dalmau

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Objective: To determine whether patients with antineuronal antibody- associated paraneoplastic cerebellar degeneration (PCD) harbor antibodies to non-N-methyl-D-aspartate glutamate receptors (GluRs) in their serum. Background: A recent study identified antibodies to GluRs in the serum of patients with PCD. Methods: Sera of 35 patients with PCD (20 anti-Yo, 5 anti- Ri, 5 anti-Tr, and 5 anti-Hu) were examined by immunohistochemistry and immunoblot techniques. The expression of GluRs was obtained after transfection of 293T cells with cDNA plasmids corresponding to GluR1, GluR2, GluR3, GluR4, GluR6, and GluR7. The tumors of four patients with anti-Yo- associated PCD and nine ovarian carcinomas from patients without PCD were examined for the expression of GluRs. Results: The expression of each GluR subunit was confirmed using affinity-purified antibodies against the corresponding GluRs and with whole sera from two rabbits immunized with GluR3. Thirty-two sera from patients with PCD were negative and 3 showed equivocal reactivity with 293T cells expressing different GluRs. None of the 35 sera had a pattern of reactivity characteristic of any GluR antibody on immunohistochemistry of sections of rat brain. Eleven of 14 tumors did not express GluRs; only some cells of one paraneoplastic tumor expressed GluRs. Conclusions: That patients with antibody-associated PCD (anti-Yo, -Ri, -Tr, and -Hu) harbor GluR antibodies in their sera is unlikely. GluR antibodies cannot be used as markers of paraneoplastic neurologic disorders.

Original languageEnglish (US)
Pages (from-to)1392-1397
Number of pages6
JournalNeurology
Volume50
Issue number5
StatePublished - May 1998
Externally publishedYes

Fingerprint

Paraneoplastic Cerebellar Degeneration
Glutamate Receptors
Antibodies
Serum
HEK293 Cells
Immunohistochemistry
D-Aspartic Acid
Neoplasms
Antibody Affinity
Nervous System Diseases
Transfection
Plasmids
Complementary DNA
Rabbits
Carcinoma

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Degenhardt, A., Duvoisin, R., Frennier, J., Gultekin, S., Rosenfeld, M. R., Posner, J. B., & Dalmau, J. (1998). Absence of antibodies to non-NMDA glutamate-receptor subunits in paraneoplastic cerebellar degeneration. Neurology, 50(5), 1392-1397.

Absence of antibodies to non-NMDA glutamate-receptor subunits in paraneoplastic cerebellar degeneration. / Degenhardt, A.; Duvoisin, Robert; Frennier, J.; Gultekin, Sakir; Rosenfeld, M. R.; Posner, J. B.; Dalmau, Josep.

In: Neurology, Vol. 50, No. 5, 05.1998, p. 1392-1397.

Research output: Contribution to journalArticle

Degenhardt, A, Duvoisin, R, Frennier, J, Gultekin, S, Rosenfeld, MR, Posner, JB & Dalmau, J 1998, 'Absence of antibodies to non-NMDA glutamate-receptor subunits in paraneoplastic cerebellar degeneration', Neurology, vol. 50, no. 5, pp. 1392-1397.
Degenhardt A, Duvoisin R, Frennier J, Gultekin S, Rosenfeld MR, Posner JB et al. Absence of antibodies to non-NMDA glutamate-receptor subunits in paraneoplastic cerebellar degeneration. Neurology. 1998 May;50(5):1392-1397.
Degenhardt, A. ; Duvoisin, Robert ; Frennier, J. ; Gultekin, Sakir ; Rosenfeld, M. R. ; Posner, J. B. ; Dalmau, Josep. / Absence of antibodies to non-NMDA glutamate-receptor subunits in paraneoplastic cerebellar degeneration. In: Neurology. 1998 ; Vol. 50, No. 5. pp. 1392-1397.
@article{503255eb4ca84ca9be621d706deba4a4,
title = "Absence of antibodies to non-NMDA glutamate-receptor subunits in paraneoplastic cerebellar degeneration",
abstract = "Objective: To determine whether patients with antineuronal antibody- associated paraneoplastic cerebellar degeneration (PCD) harbor antibodies to non-N-methyl-D-aspartate glutamate receptors (GluRs) in their serum. Background: A recent study identified antibodies to GluRs in the serum of patients with PCD. Methods: Sera of 35 patients with PCD (20 anti-Yo, 5 anti- Ri, 5 anti-Tr, and 5 anti-Hu) were examined by immunohistochemistry and immunoblot techniques. The expression of GluRs was obtained after transfection of 293T cells with cDNA plasmids corresponding to GluR1, GluR2, GluR3, GluR4, GluR6, and GluR7. The tumors of four patients with anti-Yo- associated PCD and nine ovarian carcinomas from patients without PCD were examined for the expression of GluRs. Results: The expression of each GluR subunit was confirmed using affinity-purified antibodies against the corresponding GluRs and with whole sera from two rabbits immunized with GluR3. Thirty-two sera from patients with PCD were negative and 3 showed equivocal reactivity with 293T cells expressing different GluRs. None of the 35 sera had a pattern of reactivity characteristic of any GluR antibody on immunohistochemistry of sections of rat brain. Eleven of 14 tumors did not express GluRs; only some cells of one paraneoplastic tumor expressed GluRs. Conclusions: That patients with antibody-associated PCD (anti-Yo, -Ri, -Tr, and -Hu) harbor GluR antibodies in their sera is unlikely. GluR antibodies cannot be used as markers of paraneoplastic neurologic disorders.",
author = "A. Degenhardt and Robert Duvoisin and J. Frennier and Sakir Gultekin and Rosenfeld, {M. R.} and Posner, {J. B.} and Josep Dalmau",
year = "1998",
month = "5",
language = "English (US)",
volume = "50",
pages = "1392--1397",
journal = "Neurology",
issn = "0028-3878",
publisher = "Lippincott Williams and Wilkins",
number = "5",

}

TY - JOUR

T1 - Absence of antibodies to non-NMDA glutamate-receptor subunits in paraneoplastic cerebellar degeneration

AU - Degenhardt, A.

AU - Duvoisin, Robert

AU - Frennier, J.

AU - Gultekin, Sakir

AU - Rosenfeld, M. R.

AU - Posner, J. B.

AU - Dalmau, Josep

PY - 1998/5

Y1 - 1998/5

N2 - Objective: To determine whether patients with antineuronal antibody- associated paraneoplastic cerebellar degeneration (PCD) harbor antibodies to non-N-methyl-D-aspartate glutamate receptors (GluRs) in their serum. Background: A recent study identified antibodies to GluRs in the serum of patients with PCD. Methods: Sera of 35 patients with PCD (20 anti-Yo, 5 anti- Ri, 5 anti-Tr, and 5 anti-Hu) were examined by immunohistochemistry and immunoblot techniques. The expression of GluRs was obtained after transfection of 293T cells with cDNA plasmids corresponding to GluR1, GluR2, GluR3, GluR4, GluR6, and GluR7. The tumors of four patients with anti-Yo- associated PCD and nine ovarian carcinomas from patients without PCD were examined for the expression of GluRs. Results: The expression of each GluR subunit was confirmed using affinity-purified antibodies against the corresponding GluRs and with whole sera from two rabbits immunized with GluR3. Thirty-two sera from patients with PCD were negative and 3 showed equivocal reactivity with 293T cells expressing different GluRs. None of the 35 sera had a pattern of reactivity characteristic of any GluR antibody on immunohistochemistry of sections of rat brain. Eleven of 14 tumors did not express GluRs; only some cells of one paraneoplastic tumor expressed GluRs. Conclusions: That patients with antibody-associated PCD (anti-Yo, -Ri, -Tr, and -Hu) harbor GluR antibodies in their sera is unlikely. GluR antibodies cannot be used as markers of paraneoplastic neurologic disorders.

AB - Objective: To determine whether patients with antineuronal antibody- associated paraneoplastic cerebellar degeneration (PCD) harbor antibodies to non-N-methyl-D-aspartate glutamate receptors (GluRs) in their serum. Background: A recent study identified antibodies to GluRs in the serum of patients with PCD. Methods: Sera of 35 patients with PCD (20 anti-Yo, 5 anti- Ri, 5 anti-Tr, and 5 anti-Hu) were examined by immunohistochemistry and immunoblot techniques. The expression of GluRs was obtained after transfection of 293T cells with cDNA plasmids corresponding to GluR1, GluR2, GluR3, GluR4, GluR6, and GluR7. The tumors of four patients with anti-Yo- associated PCD and nine ovarian carcinomas from patients without PCD were examined for the expression of GluRs. Results: The expression of each GluR subunit was confirmed using affinity-purified antibodies against the corresponding GluRs and with whole sera from two rabbits immunized with GluR3. Thirty-two sera from patients with PCD were negative and 3 showed equivocal reactivity with 293T cells expressing different GluRs. None of the 35 sera had a pattern of reactivity characteristic of any GluR antibody on immunohistochemistry of sections of rat brain. Eleven of 14 tumors did not express GluRs; only some cells of one paraneoplastic tumor expressed GluRs. Conclusions: That patients with antibody-associated PCD (anti-Yo, -Ri, -Tr, and -Hu) harbor GluR antibodies in their sera is unlikely. GluR antibodies cannot be used as markers of paraneoplastic neurologic disorders.

UR - http://www.scopus.com/inward/record.url?scp=0031844292&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031844292&partnerID=8YFLogxK

M3 - Article

C2 - 9595993

AN - SCOPUS:0031844292

VL - 50

SP - 1392

EP - 1397

JO - Neurology

JF - Neurology

SN - 0028-3878

IS - 5

ER -