Abnormal vascular reactivity in experimental diet-induced diabetes (C57BL/6J mice)

H. Xue, Jean-Baptiste Roullet, J. C. Chapman, C. M. Roullet, D. A. McCarron

Research output: Contribution to journalArticle

Abstract

When fed a high-fat, high-carbohydrate diet (HFC), C57BL/6J mice become obese and hypertensive, and exhibits several of the metabolic disturbances associated with non-insulin dependent (type II) diabetes: elevated glucose and insulin levels and dyslipidemia. However, the functional properties of their arteries have not been characterized Therefore, we studied the reactivity of small arteries isolated from HFC-fed (18 weeks) C57BL/6J mice by comparison with arteries isolated from normal-chow fed (NFC) animals (n=12/group). Non-susceptible mice (A/J) mice were also studied in order to determine the interaction between diet and strain. Arteries were mounted in a wire myograph and the response to norepinephrine (NE), serotonin (5-HT), acetylcholine (Ach) and Na nitroprusside (SNP) determined. The data show that HFC diet decreased significantly Ach-dependent relaxation (p2+] in fura-2 loaded arteries further showed that basal i[Ca2+] was higher (pi[Ca2+] responses to KCl and NE were significantly (p

Original languageEnglish (US)
JournalFASEB Journal
Volume11
Issue number3
StatePublished - 1997

Fingerprint

Nutrition
Medical problems
Inbred C57BL Mouse
blood vessels
arteries
experimental diets
Blood Vessels
diabetes
Arteries
Diet
Acetylcholine
Serotonin
Norepinephrine
mice
acetylcholine
norepinephrine
serotonin
Fura-2
Nitroprusside
Animals

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Xue, H., Roullet, J-B., Chapman, J. C., Roullet, C. M., & McCarron, D. A. (1997). Abnormal vascular reactivity in experimental diet-induced diabetes (C57BL/6J mice). FASEB Journal, 11(3).

Abnormal vascular reactivity in experimental diet-induced diabetes (C57BL/6J mice). / Xue, H.; Roullet, Jean-Baptiste; Chapman, J. C.; Roullet, C. M.; McCarron, D. A.

In: FASEB Journal, Vol. 11, No. 3, 1997.

Research output: Contribution to journalArticle

Xue, H, Roullet, J-B, Chapman, JC, Roullet, CM & McCarron, DA 1997, 'Abnormal vascular reactivity in experimental diet-induced diabetes (C57BL/6J mice)', FASEB Journal, vol. 11, no. 3.
Xue H, Roullet J-B, Chapman JC, Roullet CM, McCarron DA. Abnormal vascular reactivity in experimental diet-induced diabetes (C57BL/6J mice). FASEB Journal. 1997;11(3).
Xue, H. ; Roullet, Jean-Baptiste ; Chapman, J. C. ; Roullet, C. M. ; McCarron, D. A. / Abnormal vascular reactivity in experimental diet-induced diabetes (C57BL/6J mice). In: FASEB Journal. 1997 ; Vol. 11, No. 3.
@article{b3a37e6bace74e7f8a343aa81d10eee0,
title = "Abnormal vascular reactivity in experimental diet-induced diabetes (C57BL/6J mice)",
abstract = "When fed a high-fat, high-carbohydrate diet (HFC), C57BL/6J mice become obese and hypertensive, and exhibits several of the metabolic disturbances associated with non-insulin dependent (type II) diabetes: elevated glucose and insulin levels and dyslipidemia. However, the functional properties of their arteries have not been characterized Therefore, we studied the reactivity of small arteries isolated from HFC-fed (18 weeks) C57BL/6J mice by comparison with arteries isolated from normal-chow fed (NFC) animals (n=12/group). Non-susceptible mice (A/J) mice were also studied in order to determine the interaction between diet and strain. Arteries were mounted in a wire myograph and the response to norepinephrine (NE), serotonin (5-HT), acetylcholine (Ach) and Na nitroprusside (SNP) determined. The data show that HFC diet decreased significantly Ach-dependent relaxation (p2+] in fura-2 loaded arteries further showed that basal i[Ca2+] was higher (pi[Ca2+] responses to KCl and NE were significantly (p",
author = "H. Xue and Jean-Baptiste Roullet and Chapman, {J. C.} and Roullet, {C. M.} and McCarron, {D. A.}",
year = "1997",
language = "English (US)",
volume = "11",
journal = "FASEB Journal",
issn = "0892-6638",
publisher = "FASEB",
number = "3",

}

TY - JOUR

T1 - Abnormal vascular reactivity in experimental diet-induced diabetes (C57BL/6J mice)

AU - Xue, H.

AU - Roullet, Jean-Baptiste

AU - Chapman, J. C.

AU - Roullet, C. M.

AU - McCarron, D. A.

PY - 1997

Y1 - 1997

N2 - When fed a high-fat, high-carbohydrate diet (HFC), C57BL/6J mice become obese and hypertensive, and exhibits several of the metabolic disturbances associated with non-insulin dependent (type II) diabetes: elevated glucose and insulin levels and dyslipidemia. However, the functional properties of their arteries have not been characterized Therefore, we studied the reactivity of small arteries isolated from HFC-fed (18 weeks) C57BL/6J mice by comparison with arteries isolated from normal-chow fed (NFC) animals (n=12/group). Non-susceptible mice (A/J) mice were also studied in order to determine the interaction between diet and strain. Arteries were mounted in a wire myograph and the response to norepinephrine (NE), serotonin (5-HT), acetylcholine (Ach) and Na nitroprusside (SNP) determined. The data show that HFC diet decreased significantly Ach-dependent relaxation (p2+] in fura-2 loaded arteries further showed that basal i[Ca2+] was higher (pi[Ca2+] responses to KCl and NE were significantly (p

AB - When fed a high-fat, high-carbohydrate diet (HFC), C57BL/6J mice become obese and hypertensive, and exhibits several of the metabolic disturbances associated with non-insulin dependent (type II) diabetes: elevated glucose and insulin levels and dyslipidemia. However, the functional properties of their arteries have not been characterized Therefore, we studied the reactivity of small arteries isolated from HFC-fed (18 weeks) C57BL/6J mice by comparison with arteries isolated from normal-chow fed (NFC) animals (n=12/group). Non-susceptible mice (A/J) mice were also studied in order to determine the interaction between diet and strain. Arteries were mounted in a wire myograph and the response to norepinephrine (NE), serotonin (5-HT), acetylcholine (Ach) and Na nitroprusside (SNP) determined. The data show that HFC diet decreased significantly Ach-dependent relaxation (p2+] in fura-2 loaded arteries further showed that basal i[Ca2+] was higher (pi[Ca2+] responses to KCl and NE were significantly (p

UR - http://www.scopus.com/inward/record.url?scp=33750273814&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33750273814&partnerID=8YFLogxK

M3 - Article

VL - 11

JO - FASEB Journal

JF - FASEB Journal

SN - 0892-6638

IS - 3

ER -