Aberrant plasticity of peripheral sensory axons in a painful neuropathy

Takashi Hirai, Yatendra Mulpuri, Yanbing Cheng, Zheng Xia, Wei Li, Supanigar Ruangsri, Igor Spigelman, Ichiro Nishimura

Research output: Research - peer-reviewArticle

Abstract

Neuronal cells express considerable plasticity responding to environmental cues, in part, through subcellular mRNA regulation. Here we report on the extensive changes in distribution of mRNAs in the cell body and axon compartments of peripheral sensory neurons and the 3′ untranslated region (3′UTR) landscapes after unilateral sciatic nerve entrapment (SNE) injury in rats. Neuronal cells dissociated from SNE-injured and contralateral L4 and L5 dorsal root ganglia were cultured in a compartmentalized system. Axonal and cell body RNA samples were separately subjected to high throughput RNA sequencing (RNA-Seq). The injured axons exhibited enrichment of mRNAs related to protein synthesis and nerve regeneration. Lengthening of 3′UTRs was more prevalent in the injured axons, including the newly discovered alternative cleavage and polyadenylation of NaV1.8 mRNA. Alternative polyadenylation was largely independent from the relative abundance of axonal mRNAs; but they were highly clustered in functional pathways related to RNA granule formation in the injured axons. These RNA-Seq data analyses indicate that peripheral nerve injury may result in highly selective mRNA enrichment in the affected axons with 3′UTR alterations potentially contributing to the mechanism of neuropathic pain.

LanguageEnglish (US)
Article number3407
JournalScientific Reports
Volume7
Issue number1
DOIs
StatePublished - Dec 1 2017

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Axons
Messenger RNA
Painful Neuropathy
3' Untranslated Regions
Nerve Compression Syndromes
Polyadenylation
Sciatic Nerve
RNA
Cell Body
RNA Sequence Analysis
High-Throughput Nucleotide Sequencing
Peripheral Nerve Injuries
Nerve Regeneration
Spinal Ganglia
Neuralgia
Sensory Receptor Cells
Cues
Wounds and Injuries
Proteins

ASJC Scopus subject areas

  • General

Cite this

Hirai, T., Mulpuri, Y., Cheng, Y., Xia, Z., Li, W., Ruangsri, S., ... Nishimura, I. (2017). Aberrant plasticity of peripheral sensory axons in a painful neuropathy. Scientific Reports, 7(1), [3407]. DOI: 10.1038/s41598-017-03390-9

Aberrant plasticity of peripheral sensory axons in a painful neuropathy. / Hirai, Takashi; Mulpuri, Yatendra; Cheng, Yanbing; Xia, Zheng; Li, Wei; Ruangsri, Supanigar; Spigelman, Igor; Nishimura, Ichiro.

In: Scientific Reports, Vol. 7, No. 1, 3407, 01.12.2017.

Research output: Research - peer-reviewArticle

Hirai, T, Mulpuri, Y, Cheng, Y, Xia, Z, Li, W, Ruangsri, S, Spigelman, I & Nishimura, I 2017, 'Aberrant plasticity of peripheral sensory axons in a painful neuropathy' Scientific Reports, vol 7, no. 1, 3407. DOI: 10.1038/s41598-017-03390-9
Hirai T, Mulpuri Y, Cheng Y, Xia Z, Li W, Ruangsri S et al. Aberrant plasticity of peripheral sensory axons in a painful neuropathy. Scientific Reports. 2017 Dec 1;7(1). 3407. Available from, DOI: 10.1038/s41598-017-03390-9
Hirai, Takashi ; Mulpuri, Yatendra ; Cheng, Yanbing ; Xia, Zheng ; Li, Wei ; Ruangsri, Supanigar ; Spigelman, Igor ; Nishimura, Ichiro. / Aberrant plasticity of peripheral sensory axons in a painful neuropathy. In: Scientific Reports. 2017 ; Vol. 7, No. 1.
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