Aberrant, persistent inclusion into lipid rafts limits the tumorigenic function of membrane type-1 matrix metalloproteinase in malignant cells

Dmitri Rozanov, Elena I. Deryugina, Edward Z. Monosov, Natalia D. Marchenko, Alex Y. Strongin

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

Membrane type-1 matrix metalloproteinase (MT1-MMP) is a key enzyme in cell locomotion and tissue remodeling. Trafficking to the plasma membrane and internalization into the transient storage compartment both regulate the cell surface presentation of MT1-MMP. Our data indicate that mutant MT1-MMP lacking the cytoplasmic tail is recruited to the caveolae-enriched lipid raft membrane microdomains in breast carcinoma MCF7 cells. In contrast, the wild-type protease is not permanently associated with lipid rafts. Trafficking to lipid rafts correlated with poor internalization and the persistent presentation of MT1-MMP at the cell surface. The tail mutant efficiently functioned in inducing the activation of the latent proMMP-2 zymogen, matrix remodeling, and contraction of three-dimensional collagen lattices. Recruitment of the tail mutant to lipid raft antagonized, however, the cleavage of the plasma membrane-associated E-cadherin. These events limited the contribution of the tail mutant to cell locomotion and malignant growth. It is conceivable that the tail peptide sequence plays a crucial role in the translocations of MT1-MMP across the cell and contributes to coordinated cellular functions. It is tempting to hypothesize that the mechanisms involved in trafficking of MT1-MMP to caveolin-enriched lipid rafts may be targeted in a clinically advantageous manner.

Original languageEnglish (US)
Pages (from-to)81-95
Number of pages15
JournalExperimental Cell Research
Volume293
Issue number1
DOIs
StatePublished - Feb 1 2004
Externally publishedYes

Fingerprint

Matrix Metalloproteinase 14
Lipids
Cell Movement
Cell Membrane
Membrane Microdomains
Caveolins
Caveolae
Enzyme Precursors
MCF-7 Cells
Cadherins
Peptide Hydrolases
Collagen
Breast Neoplasms
Peptides
Enzymes
Growth

Keywords

  • Breast carcinoma
  • Caveolin
  • Extracellular matrix
  • Glioblastoma
  • Invasion
  • Lipid rafts
  • Migration
  • Xenografts

ASJC Scopus subject areas

  • Cell Biology

Cite this

Aberrant, persistent inclusion into lipid rafts limits the tumorigenic function of membrane type-1 matrix metalloproteinase in malignant cells. / Rozanov, Dmitri; Deryugina, Elena I.; Monosov, Edward Z.; Marchenko, Natalia D.; Strongin, Alex Y.

In: Experimental Cell Research, Vol. 293, No. 1, 01.02.2004, p. 81-95.

Research output: Contribution to journalArticle

Rozanov, Dmitri ; Deryugina, Elena I. ; Monosov, Edward Z. ; Marchenko, Natalia D. ; Strongin, Alex Y. / Aberrant, persistent inclusion into lipid rafts limits the tumorigenic function of membrane type-1 matrix metalloproteinase in malignant cells. In: Experimental Cell Research. 2004 ; Vol. 293, No. 1. pp. 81-95.
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