AAF-cmk sensitizes tumor cells to trail-mediated apoptosis

Izabela Młnarczuk, Paweł Mróz, Grazyna Hoser, Dominika Nowis, Łukasz P. Biały, Halina Ziemba, Tomasz Grzela, Wojciech Feleszko, Jacek Malejczyk, Cezary Wojcik, Marek Jakóbisiak, Jakub Goła̧b

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

TRAIL is a member of the tumor necrosis factor (TNF) superfamily. This cytokine is cytotoxic for a high proportion of tumor cells, but could be also toxic for normal cells. There is a need to find other agents able to potentiate the antitumor effects of this cytokine. In our study, we found that Ala-Ala-Phe-chloromethylketone (AAF-cmk) augmented cytotoxic activity of TRAIL or TNF against human leukemic cells. Flow cytometry studies and electron microscopy revealed that apoptosis was primarily responsible for this potentiation. Altogether, our studies indicate that AAF-cmk might effectively sensitize human leukemia cells to apoptosis induced by TRAIL and TNF.

Original languageEnglish (US)
Pages (from-to)53-61
Number of pages9
JournalLeukemia Research
Volume28
Issue number1
DOIs
StatePublished - Jan 2004
Externally publishedYes

Fingerprint

Apoptosis
Neoplasms
Tumor Necrosis Factor-alpha
Cytokines
Poisons
Electron Microscopy
Flow Cytometry
Leukemia
alanyl-alanyl-phenylalanine chloromethyl ketone
human TNF protein

Keywords

  • AAF-cmk
  • Apoptosis
  • Lymphoma
  • TNF
  • TRAIL
  • Tripeptidylpeptidase II

ASJC Scopus subject areas

  • Cancer Research
  • Hematology
  • Oncology

Cite this

Młnarczuk, I., Mróz, P., Hoser, G., Nowis, D., Biały, Ł. P., Ziemba, H., ... Goła̧b, J. (2004). AAF-cmk sensitizes tumor cells to trail-mediated apoptosis. Leukemia Research, 28(1), 53-61. https://doi.org/10.1016/S0145-2126(03)00122-X

AAF-cmk sensitizes tumor cells to trail-mediated apoptosis. / Młnarczuk, Izabela; Mróz, Paweł; Hoser, Grazyna; Nowis, Dominika; Biały, Łukasz P.; Ziemba, Halina; Grzela, Tomasz; Feleszko, Wojciech; Malejczyk, Jacek; Wojcik, Cezary; Jakóbisiak, Marek; Goła̧b, Jakub.

In: Leukemia Research, Vol. 28, No. 1, 01.2004, p. 53-61.

Research output: Contribution to journalArticle

Młnarczuk, I, Mróz, P, Hoser, G, Nowis, D, Biały, ŁP, Ziemba, H, Grzela, T, Feleszko, W, Malejczyk, J, Wojcik, C, Jakóbisiak, M & Goła̧b, J 2004, 'AAF-cmk sensitizes tumor cells to trail-mediated apoptosis', Leukemia Research, vol. 28, no. 1, pp. 53-61. https://doi.org/10.1016/S0145-2126(03)00122-X
Młnarczuk I, Mróz P, Hoser G, Nowis D, Biały ŁP, Ziemba H et al. AAF-cmk sensitizes tumor cells to trail-mediated apoptosis. Leukemia Research. 2004 Jan;28(1):53-61. https://doi.org/10.1016/S0145-2126(03)00122-X
Młnarczuk, Izabela ; Mróz, Paweł ; Hoser, Grazyna ; Nowis, Dominika ; Biały, Łukasz P. ; Ziemba, Halina ; Grzela, Tomasz ; Feleszko, Wojciech ; Malejczyk, Jacek ; Wojcik, Cezary ; Jakóbisiak, Marek ; Goła̧b, Jakub. / AAF-cmk sensitizes tumor cells to trail-mediated apoptosis. In: Leukemia Research. 2004 ; Vol. 28, No. 1. pp. 53-61.
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