A yeast origin of replication is activated late in S phase

Betsy Ferguson, Bonita J. Brewer, Ann E. Reynolds, Walton L. Fangman

Research output: Contribution to journalArticle

150 Citations (Scopus)

Abstract

The mechanism that causes large regions of eukaryotic chromosomes to remain unreplicated until late in S phase is not understood. We have found that 67 kb of telomere-adjacent DNA at the right end of chromosome V in S. cerevisiae is replicated late in S phase. An ARS element in this region, ARS501, was shown by two-dimensional gel analysis to be an active origin of replication. Kinetic analyses indicate that the rate of replication fork movement within this late region is similar to that in early replicating regions. Therefore, the delayed replication of the region is a consequence of late origin activation. The results also support the idea that the pattern of interspersed early and late replication along the chromosomes of higher eukaryotes is a consequence of the temporal regulation of origin activation.

Original languageEnglish (US)
Pages (from-to)507-515
Number of pages9
JournalCell
Volume65
Issue number3
DOIs
StatePublished - May 3 1991
Externally publishedYes

Fingerprint

Replication Origin
Chromosomes
S Phase
Yeast
Yeasts
Chemical activation
Telomere
Eukaryota
Saccharomyces cerevisiae
Gels
Kinetics
DNA

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Ferguson, B., Brewer, B. J., Reynolds, A. E., & Fangman, W. L. (1991). A yeast origin of replication is activated late in S phase. Cell, 65(3), 507-515. https://doi.org/10.1016/0092-8674(91)90468-E

A yeast origin of replication is activated late in S phase. / Ferguson, Betsy; Brewer, Bonita J.; Reynolds, Ann E.; Fangman, Walton L.

In: Cell, Vol. 65, No. 3, 03.05.1991, p. 507-515.

Research output: Contribution to journalArticle

Ferguson, B, Brewer, BJ, Reynolds, AE & Fangman, WL 1991, 'A yeast origin of replication is activated late in S phase', Cell, vol. 65, no. 3, pp. 507-515. https://doi.org/10.1016/0092-8674(91)90468-E
Ferguson, Betsy ; Brewer, Bonita J. ; Reynolds, Ann E. ; Fangman, Walton L. / A yeast origin of replication is activated late in S phase. In: Cell. 1991 ; Vol. 65, No. 3. pp. 507-515.
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