A xeroderma pigmentosum complementation group A related gene: confirmation using monoclonal antibodies against the cyclobutane dimer and (6-4) photoproduct

T. Mori, T. L. Rinaldy, R. S. Athwal, G. P. Kaur, O. Nikaido, R. S. Lloyd, A. Rinaldy

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

Xeroderma pigmentosum complementation group A was partially complemented by a cosmid genomic clone containing a 42-kb human DNA insert selected with a cDNA clone that we obtained through cDNA competition between the repair-proficient and repair-deficient cell line. The relationship between these two clones was confirmed using PCR amplifications. The enhancement in DNA-repair capacity of the transformants was assessed with the monoclonal antibodies specific for cyclobutane dimers and (6-4) photoproducts and partially correct the xeroderma pigmentosum complementation group A defect. Furthermore, the level of the photoproduct-repair capacity is in agreement with the survival enhancement calculated from the D37 values. This gene was mapped to chromosome 8, suggesting that this may represent one of the defective gene(s) in xeroderma pigmentosum complementation group A.

Original languageEnglish (US)
Pages (from-to)143-150
Number of pages8
JournalMutation Research-DNA Repair
Volume293
Issue number2
DOIs
StatePublished - Jan 1993

Keywords

  • (6-4) Photoproduct
  • Complementation group A
  • Cyclobutane dimer
  • DNA-repair gene
  • Xeroderma pigmentosum

ASJC Scopus subject areas

  • Molecular Biology
  • Toxicology
  • Genetics

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