A virus-like particle enzyme-linked immunosorbent assay detects serum antibodies in a majority of women infected with human papillomavirus type 16

Reinhard Kirnbauer, Nancy L. Hubbert, Cosette M. Wheeler, Thomas Becker, Douglas R. Lowy, John T. Schiller

Research output: Contribution to journalArticle

319 Citations (Scopus)

Abstract

Background: Previous studies have demonstrated that genital infection with high-risk types of human papillomavirus (HPV), most often HPV16, is the most significant risk factor for the development of cervical cancer. However, serologic assays that have been developed to identify high-risk HPV infection have either failed to associate serum reactivity with other indicators of HPV infection or have identified only a minority of HPV-infected individuals. Purpose: Our purpose was to determine whether a specifically developed enzyme-linked immunosorbent assay (ELISA) could detect IgG anti-HPV16 virion antibodies in the sera of women who had tested positive for genital HPV16 infection by DNA-based methods. Methods: An ELISA was developed using newly developed HPV16 virus-like particles as antigens to detect anti-HPV16 virion IgG antibodies. These particles are comprised of HPV16 structural proteins that are self-assembled in insect cells after expression by recombinant baculoviruses. The sera of 122 women, whose HPV status had been previously evaluated by nucleic acid-based methods, were tested by this ELISA. Results: The sera of 59% of women (32 of 54) positive for genital HPV16 DNA by polymerase chain reaction (PCR) were positive in the ELISA assay compared with sera from women who had tested negative for HPV DNA (P

Original languageEnglish (US)
Pages (from-to)494-499
Number of pages6
JournalJournal of the National Cancer Institute
Volume86
Issue number7
StatePublished - Apr 6 1994
Externally publishedYes

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Enzyme-linked Immunosorbent Assay
Human papillomavirus 16
Viruses
Antibody
Antibodies
Virion
Virus
Assays
Enzymes
Enzyme-Linked Immunosorbent Assay
Infection
Serum
DNA
Papillomavirus Infections
Polymerase Chain Reaction
Baculoviridae
Polymerase chain reaction
Nucleic acids
Reactivity
DNA-Directed DNA Polymerase

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Physiology (medical)
  • Radiology Nuclear Medicine and imaging

Cite this

A virus-like particle enzyme-linked immunosorbent assay detects serum antibodies in a majority of women infected with human papillomavirus type 16. / Kirnbauer, Reinhard; Hubbert, Nancy L.; Wheeler, Cosette M.; Becker, Thomas; Lowy, Douglas R.; Schiller, John T.

In: Journal of the National Cancer Institute, Vol. 86, No. 7, 06.04.1994, p. 494-499.

Research output: Contribution to journalArticle

Kirnbauer, Reinhard ; Hubbert, Nancy L. ; Wheeler, Cosette M. ; Becker, Thomas ; Lowy, Douglas R. ; Schiller, John T. / A virus-like particle enzyme-linked immunosorbent assay detects serum antibodies in a majority of women infected with human papillomavirus type 16. In: Journal of the National Cancer Institute. 1994 ; Vol. 86, No. 7. pp. 494-499.
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