A viral inhibitor of peptide transporters for antigen presentation

Klaus Früh, Kwangseog Ahn, Hakim Djaballah, Pascal Sempé, Peter M. van Endert, Robert Tampé, Per A. Peterson, Young Yang

Research output: Contribution to journalLetter

491 Scopus citations

Abstract

CYTOTOXIC T lymphocytes lyse target cells after T-cell-receptor-mediated recognition of class I major histocompatibility complex molecules presenting peptides1. Antigenic peptides are generated in the cytoplasm by proteasomes2 and translocated into the lumen of the endoplasmic reticulum (ER) by peptide transporters (TAP)3-6. Herpes simplex virus (HSV) expresses a cytoplasmic protein, ICP47, which seems to interfere with such immune surveillance by mediating retention of 'empty' class I molecules in the ER7, 8. By expressing ICP47 in HeLa cells under an inducible promoter9, we show that ICP47 efficiently inhibits peptide transport across the ER membrane such that nascent class I molecules fail to acquire antigenic peptides. This inhibition was overcome by transfecting murine TAP. Further, we demonstrate that ICP47 colocalizes and physically associates with TAP within the cell. Inhibition of peptide translocation by a viral protein indicates a previously undocumented potential mechanism for viral immune evasion.

Original languageEnglish (US)
Pages (from-to)415-418
Number of pages4
JournalNature
Volume375
Issue number6530
DOIs
StatePublished - Jun 1 1995

ASJC Scopus subject areas

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    Früh, K., Ahn, K., Djaballah, H., Sempé, P., van Endert, P. M., Tampé, R., Peterson, P. A., & Yang, Y. (1995). A viral inhibitor of peptide transporters for antigen presentation. Nature, 375(6530), 415-418. https://doi.org/10.1038/375415a0