A unique kex2-like endoprotease from drosophila melanogaster is expressed in the central nervous system during early embryogenesis

Joel S. Hayflick, William J. Wolfgang, Michael Forte, Gary Thomas

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Complementary DNA sequences were cloned from a Drosophila library encoding a 1101 amino acid polypeptide that we have named dKLIP-1. The deduced protein is structurally similar to the yeast KEX2 prohormone endoprotease including the conserved Asp, His, and Ser catalytic triad residues characteristic of the subtilisin family. When coexpressed in vivo with pro-β-NGF, dKLIP-1 greatly enhanced the endoproteolytic conversion of the precursor to mature β-NGF by cleavage at a -Lys-Arg- doublet. In adults, dKLIP-1 transcripts were detected in cortical regions of the CNS and fat body. Most striking, however, was the high level of maternal transcripts deposited into developing oocytes. The temporal and spatial expression of dKLIP-1 mRNAs during embryonic development indicates a potential role for this novel Kex2p-like endoprotease in early embryogenesis and neurogenesis.

Original languageEnglish (US)
Pages (from-to)705-717
Number of pages13
JournalJournal of Neuroscience
Volume12
Issue number3
StatePublished - 1992

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Nerve Growth Factor
Drosophila melanogaster
Embryonic Development
Central Nervous System
Subtilisin
Fat Body
Neurogenesis
Libraries
Drosophila
Oocytes
Complementary DNA
Yeasts
Mothers
Amino Acids
Messenger RNA
Peptides
Proteins

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

A unique kex2-like endoprotease from drosophila melanogaster is expressed in the central nervous system during early embryogenesis. / Hayflick, Joel S.; Wolfgang, William J.; Forte, Michael; Thomas, Gary.

In: Journal of Neuroscience, Vol. 12, No. 3, 1992, p. 705-717.

Research output: Contribution to journalArticle

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