A two-step adhesion cascade for T cell/endothelial cell interactions under flow conditions

David A. Jones, Larry V. McIntire, C. Wayne Smith, Louis Picker

Research output: Contribution to journalArticle

151 Citations (Scopus)

Abstract

Neutrophil adherence to endothelial cells (ECs) under conditions of flow occurs in successive steps, including selectin-dependent primary adhesion and CD18-dependent secondary adhesion. We used a parallel-plate flow chamber to assess the steps in T cell adherence in vitro. On monolayers of L cells transfected with the EC adhesion molecules E-selectin, vascular cell adhesion molecule-1 (VCAM-1), or intercellular adhesion molecule-1 (ICAM-1), E- selectin was capable of mediating only primary adhesion, ICAM-1 was capable of mediating only secondary adhesion, and VCAM-1 was capable of mediating both primary and secondary adhesion. Studies using human umbilical vein EC monolayers stimulated for 24 h with IL-1 also revealed distinct primary and secondary steps in T cell adhesion under flow, and the secondary adhesion was inhibited > 90% by blocking both VCAM-1/α4β1 integrin and ICAM-1/CD18 integrin pathways. However, the primary adhesion under conditions of flow could not be attributed to any of the mechanisms known to support adhesion of leukocytes to ECs. Alone, this pathway was shown to mediate T cell rolling and was a necessary prerequisite for engagement of the two integrin pathways in this system. Thus, T cell adherence to 24-h IL-1-stimulated human umbilical vein ECs at venular wall shear stresses involves at least two successive steps, with clear molecular distinctions from the mechanisms accounting for neutrophil/EC adhesion.

Original languageEnglish (US)
Pages (from-to)2443-2450
Number of pages8
JournalJournal of Clinical Investigation
Volume94
Issue number6
StatePublished - Dec 1994
Externally publishedYes

Fingerprint

Cell Communication
Vascular Cell Adhesion Molecule-1
Intercellular Adhesion Molecule-1
Endothelial Cells
Integrins
T-Lymphocytes
E-Selectin
Human Umbilical Vein Endothelial Cells
Interleukin-1
Cell Adhesion
Neutrophils
Selectins
Cell Adhesion Molecules
Leukocytes

Keywords

  • cell adhesion molecules
  • interleukin- 1
  • lymphocytes
  • monoclonal antibodies
  • vascular endothelium

ASJC Scopus subject areas

  • Medicine(all)

Cite this

A two-step adhesion cascade for T cell/endothelial cell interactions under flow conditions. / Jones, David A.; McIntire, Larry V.; Smith, C. Wayne; Picker, Louis.

In: Journal of Clinical Investigation, Vol. 94, No. 6, 12.1994, p. 2443-2450.

Research output: Contribution to journalArticle

Jones, David A. ; McIntire, Larry V. ; Smith, C. Wayne ; Picker, Louis. / A two-step adhesion cascade for T cell/endothelial cell interactions under flow conditions. In: Journal of Clinical Investigation. 1994 ; Vol. 94, No. 6. pp. 2443-2450.
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