TY - JOUR
T1 - A triggered mechanism retrieves membrane in seconds after Ca2+- stimulated exocytosis in single pituitary cells
AU - Thomas, P.
AU - Lee, A. K.
AU - Wong, J. G.
AU - Almers, W.
PY - 1994
Y1 - 1994
N2 - In neuroendocrine cells, cytosolic Ca2+ triggers exocytosis in tens of milliseconds, yet known pathways of endocytic membrane retrieval take minutes. To test for faster retrieval mechanisms, we have triggered short bursts of exocytosis by flash photolysis of caged Ca2+, and have tracked subsequent retrieval by measuring the plasma membrane capacitance. We find that a limited amount of membrane can be retrieved with a time constant of 4 s at 21-26°C, and that this occurs partially via structures larger than coated vesicles. This novel mechanism may be arrested at a late step. Incomplete retrieval structures then remain on the cell surface for minutes until the consequences of a renewed increase in cytosolic [Ca2+] disconnect them from the cell surface in <1 s. Our results provide evidence for a rapid, triggered membrane retrieval pathway in excitable cells.
AB - In neuroendocrine cells, cytosolic Ca2+ triggers exocytosis in tens of milliseconds, yet known pathways of endocytic membrane retrieval take minutes. To test for faster retrieval mechanisms, we have triggered short bursts of exocytosis by flash photolysis of caged Ca2+, and have tracked subsequent retrieval by measuring the plasma membrane capacitance. We find that a limited amount of membrane can be retrieved with a time constant of 4 s at 21-26°C, and that this occurs partially via structures larger than coated vesicles. This novel mechanism may be arrested at a late step. Incomplete retrieval structures then remain on the cell surface for minutes until the consequences of a renewed increase in cytosolic [Ca2+] disconnect them from the cell surface in <1 s. Our results provide evidence for a rapid, triggered membrane retrieval pathway in excitable cells.
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U2 - 10.1083/jcb.124.5.667
DO - 10.1083/jcb.124.5.667
M3 - Article
C2 - 8120090
AN - SCOPUS:0028299459
VL - 124
SP - 667
EP - 675
JO - Journal of Cell Biology
JF - Journal of Cell Biology
SN - 0021-9525
IS - 5
ER -