A triggered mechanism retrieves membrane in seconds after Ca2+- stimulated exocytosis in single pituitary cells

P. Thomas, A. K. Lee, J. G. Wong, W. Almers

Research output: Contribution to journalArticlepeer-review

151 Scopus citations

Abstract

In neuroendocrine cells, cytosolic Ca2+ triggers exocytosis in tens of milliseconds, yet known pathways of endocytic membrane retrieval take minutes. To test for faster retrieval mechanisms, we have triggered short bursts of exocytosis by flash photolysis of caged Ca2+, and have tracked subsequent retrieval by measuring the plasma membrane capacitance. We find that a limited amount of membrane can be retrieved with a time constant of 4 s at 21-26°C, and that this occurs partially via structures larger than coated vesicles. This novel mechanism may be arrested at a late step. Incomplete retrieval structures then remain on the cell surface for minutes until the consequences of a renewed increase in cytosolic [Ca2+] disconnect them from the cell surface in <1 s. Our results provide evidence for a rapid, triggered membrane retrieval pathway in excitable cells.

Original languageEnglish (US)
Pages (from-to)667-675
Number of pages9
JournalJournal of Cell Biology
Volume124
Issue number5
DOIs
StatePublished - 1994

ASJC Scopus subject areas

  • Cell Biology

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