A Transgenic Marker for Newly Born Granule Cells in Dentate Gyrus

Linda S. Overstreet, Shane T. Hentges, Viviana F. Bumaschny, Flavio S J De Souza, James L. Smart, Andrea M. Santangelo, Malcolm J. Low, Gary Westbrook, Marcelo Rubinstein

Research output: Contribution to journalArticle

151 Citations (Scopus)

Abstract

Neurogenesis in the dentate gyrus continues into adulthood, yet little is known about the function of newly born neurons or how they integrate into an existing network of mature neurons. We made transgenic mice that selectively and transiently express enhanced green fluorescent protein (EGFP) in newly born granule cells of the dentate gyrus under the transcriptional control of proopiomelanocortin (POMC) genomic sequences. Analysis of transgenic pedigrees with truncation or deletion mutations indicated that EGFP expression in the dentate gyrus required cryptic POMC promoter regions dispensable for arcuate hypothalamic or pituitary expression. Unlike arcuate neurons, dentate granule cells did not express the endogenous POMC gene. EGFP-positive neurons had immature properties, including short spineless dendrites and small action potentials. Colocalization with bromodeoxyuridine indicated that EGFP-labeled granule cells were ∼2 weeks postmitotic. EGFP-labeled cells expressed markers for immature granule cells but not the glial marker GFAP. The number of EGFP-labeled neurons declined with age and increased with exercise, paralleling neurogenesis. Our results indicate that POMC-EGFP marks immature granule cells and that adult-generated granule cells integrate quite slowly into the hippocampal circuitry.

Original languageEnglish (US)
Pages (from-to)3251-3259
Number of pages9
JournalJournal of Neuroscience
Volume24
Issue number13
DOIs
StatePublished - Mar 31 2004

Fingerprint

Dentate Gyrus
Pro-Opiomelanocortin
Neurons
Neurogenesis
Sequence Deletion
Bromodeoxyuridine
Pedigree
Dendrites
enhanced green fluorescent protein
Genetic Promoter Regions
Neuroglia
Transgenic Mice
Action Potentials
Genes

Keywords

  • BrdU
  • Dentate granule cell
  • Green fluorescent protein
  • Neurogenesis
  • Neuronal progenitor
  • Proopiomelanocortin
  • PSA-nCAM
  • Transgenic mice

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Overstreet, L. S., Hentges, S. T., Bumaschny, V. F., De Souza, F. S. J., Smart, J. L., Santangelo, A. M., ... Rubinstein, M. (2004). A Transgenic Marker for Newly Born Granule Cells in Dentate Gyrus. Journal of Neuroscience, 24(13), 3251-3259. https://doi.org/10.1523/JNEUROSCI.5173-03.2004

A Transgenic Marker for Newly Born Granule Cells in Dentate Gyrus. / Overstreet, Linda S.; Hentges, Shane T.; Bumaschny, Viviana F.; De Souza, Flavio S J; Smart, James L.; Santangelo, Andrea M.; Low, Malcolm J.; Westbrook, Gary; Rubinstein, Marcelo.

In: Journal of Neuroscience, Vol. 24, No. 13, 31.03.2004, p. 3251-3259.

Research output: Contribution to journalArticle

Overstreet, LS, Hentges, ST, Bumaschny, VF, De Souza, FSJ, Smart, JL, Santangelo, AM, Low, MJ, Westbrook, G & Rubinstein, M 2004, 'A Transgenic Marker for Newly Born Granule Cells in Dentate Gyrus', Journal of Neuroscience, vol. 24, no. 13, pp. 3251-3259. https://doi.org/10.1523/JNEUROSCI.5173-03.2004
Overstreet LS, Hentges ST, Bumaschny VF, De Souza FSJ, Smart JL, Santangelo AM et al. A Transgenic Marker for Newly Born Granule Cells in Dentate Gyrus. Journal of Neuroscience. 2004 Mar 31;24(13):3251-3259. https://doi.org/10.1523/JNEUROSCI.5173-03.2004
Overstreet, Linda S. ; Hentges, Shane T. ; Bumaschny, Viviana F. ; De Souza, Flavio S J ; Smart, James L. ; Santangelo, Andrea M. ; Low, Malcolm J. ; Westbrook, Gary ; Rubinstein, Marcelo. / A Transgenic Marker for Newly Born Granule Cells in Dentate Gyrus. In: Journal of Neuroscience. 2004 ; Vol. 24, No. 13. pp. 3251-3259.
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