A trans-acting regulatory gene that inversely affects the expression of the white, brown and scarlet loci in Drosophila

L. Rabinow, Anh Nguyen-Huynh, J. A. Birchler

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

A trans-acting regulatory gene, Inr-a, that alters the level of expression of the white eye color locus as an inverse function of the number of its functional copies is described. Several independent lines of evidence demonstrate that this regulatory gene interacts with white via the promoter sequences. Among these are the observations that the inverse regulatory effect is conferred to the Adh gene when fused to the white promoter and that cis-regulatory mutants of white fail to respond. The phenotypic response to Inr-a is found in all tissues in which white is expressed, and mutants of the regulator exhibit a recessive lethality during larval periods. Increased white messenger RNA levels in pupal stages are found in Inr-a/+ individuals versus +/+ and a coordinate response is observed for mRNA levels from the brown and scarlet loci. All are structurally related and participate in pigment deposition. These experiments demonstrate that a single regulatory gene can exert an inverse effect on a target structural locus, a situation postulated from segmental aneuploid studies of gene expression and dosage compensation.

Original languageEnglish (US)
Pages (from-to)463-480
Number of pages18
JournalGenetics
Volume129
Issue number2
StatePublished - 1991
Externally publishedYes

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Regulator Genes
Drosophila
Eye Color
Messenger RNA
Aneuploidy
Gene Expression
Genes

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

A trans-acting regulatory gene that inversely affects the expression of the white, brown and scarlet loci in Drosophila. / Rabinow, L.; Nguyen-Huynh, Anh; Birchler, J. A.

In: Genetics, Vol. 129, No. 2, 1991, p. 463-480.

Research output: Contribution to journalArticle

Rabinow, L. ; Nguyen-Huynh, Anh ; Birchler, J. A. / A trans-acting regulatory gene that inversely affects the expression of the white, brown and scarlet loci in Drosophila. In: Genetics. 1991 ; Vol. 129, No. 2. pp. 463-480.
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