A-to-I RNA Editing Contributes to Proteomic Diversity in Cancer

Xinxin Peng, Xiaoyan Xu, Yumeng Wang, David H. Hawke, Shuangxing Yu, Leng Han, Zhicheng Zhou, Kamalika Mojumdar, Kang Jin Jeong, Marilyne Labrie, Yiu Huen Tsang, Minying Zhang, Yiling Lu, Patrick Hwu, Kenneth L. Scott, Han Liang, Gordon Mills

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Adenosine (A) to inosine (I) RNA editing introduces many nucleotide changes in cancer transcriptomes. However, due to the complexity of post-transcriptional regulation, the contribution of RNA editing to proteomic diversity in human cancers remains unclear. Here, we performed an integrated analysis of TCGA genomic data and CPTAC proteomic data. Despite limited site diversity, we demonstrate that A-to-I RNA editing contributes to proteomic diversity in breast cancer through changes in amino acid sequences. We validate the presence of editing events at both RNA and protein levels. The edited COPA protein increases proliferation, migration, and invasion of cancer cells in vitro. Our study suggests an important contribution of A-to-I RNA editing to protein diversity in cancer and highlights its translational potential. By an integrated analysis of TCGA genomic data and CPTAC proteomic data, Peng et al. show that A-to-I RNA editing contributes to proteomic diversity in breast cancer through changes in amino acid sequences. The edited COPA protein increases proliferation, migration, and invasion of cancer cells in vitro.

Original languageEnglish (US)
Pages (from-to)817-828.e7
JournalCancer Cell
Volume33
Issue number5
DOIs
StatePublished - May 14 2018
Externally publishedYes

Fingerprint

RNA Editing
Proteomics
Neoplasms
Amino Acid Sequence
Proteins
Breast Neoplasms
Inosine
Transcriptome
Adenosine
Nucleotides
immune RNA
RNA

Keywords

  • ADAR enzyme
  • amino acid changes
  • biomarker
  • cancer drivers
  • CPTAC
  • mass spectrometry data
  • RNA editing
  • RNA-seq
  • somatic mutations
  • TCGA

ASJC Scopus subject areas

  • Oncology
  • Cell Biology
  • Cancer Research

Cite this

Peng, X., Xu, X., Wang, Y., Hawke, D. H., Yu, S., Han, L., ... Mills, G. (2018). A-to-I RNA Editing Contributes to Proteomic Diversity in Cancer. Cancer Cell, 33(5), 817-828.e7. https://doi.org/10.1016/j.ccell.2018.03.026

A-to-I RNA Editing Contributes to Proteomic Diversity in Cancer. / Peng, Xinxin; Xu, Xiaoyan; Wang, Yumeng; Hawke, David H.; Yu, Shuangxing; Han, Leng; Zhou, Zhicheng; Mojumdar, Kamalika; Jeong, Kang Jin; Labrie, Marilyne; Tsang, Yiu Huen; Zhang, Minying; Lu, Yiling; Hwu, Patrick; Scott, Kenneth L.; Liang, Han; Mills, Gordon.

In: Cancer Cell, Vol. 33, No. 5, 14.05.2018, p. 817-828.e7.

Research output: Contribution to journalArticle

Peng, X, Xu, X, Wang, Y, Hawke, DH, Yu, S, Han, L, Zhou, Z, Mojumdar, K, Jeong, KJ, Labrie, M, Tsang, YH, Zhang, M, Lu, Y, Hwu, P, Scott, KL, Liang, H & Mills, G 2018, 'A-to-I RNA Editing Contributes to Proteomic Diversity in Cancer', Cancer Cell, vol. 33, no. 5, pp. 817-828.e7. https://doi.org/10.1016/j.ccell.2018.03.026
Peng X, Xu X, Wang Y, Hawke DH, Yu S, Han L et al. A-to-I RNA Editing Contributes to Proteomic Diversity in Cancer. Cancer Cell. 2018 May 14;33(5):817-828.e7. https://doi.org/10.1016/j.ccell.2018.03.026
Peng, Xinxin ; Xu, Xiaoyan ; Wang, Yumeng ; Hawke, David H. ; Yu, Shuangxing ; Han, Leng ; Zhou, Zhicheng ; Mojumdar, Kamalika ; Jeong, Kang Jin ; Labrie, Marilyne ; Tsang, Yiu Huen ; Zhang, Minying ; Lu, Yiling ; Hwu, Patrick ; Scott, Kenneth L. ; Liang, Han ; Mills, Gordon. / A-to-I RNA Editing Contributes to Proteomic Diversity in Cancer. In: Cancer Cell. 2018 ; Vol. 33, No. 5. pp. 817-828.e7.
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abstract = "Adenosine (A) to inosine (I) RNA editing introduces many nucleotide changes in cancer transcriptomes. However, due to the complexity of post-transcriptional regulation, the contribution of RNA editing to proteomic diversity in human cancers remains unclear. Here, we performed an integrated analysis of TCGA genomic data and CPTAC proteomic data. Despite limited site diversity, we demonstrate that A-to-I RNA editing contributes to proteomic diversity in breast cancer through changes in amino acid sequences. We validate the presence of editing events at both RNA and protein levels. The edited COPA protein increases proliferation, migration, and invasion of cancer cells in vitro. Our study suggests an important contribution of A-to-I RNA editing to protein diversity in cancer and highlights its translational potential. By an integrated analysis of TCGA genomic data and CPTAC proteomic data, Peng et al. show that A-to-I RNA editing contributes to proteomic diversity in breast cancer through changes in amino acid sequences. The edited COPA protein increases proliferation, migration, and invasion of cancer cells in vitro.",
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