A tissue-engineered 3D model of light scattering in atherosclerotic plaques

David Levitz, Monica Hinds, Ruikang K. Wang, Ma Zhenhe, Katsu Ishii, Noi Tran, Owen McCarty, Stephen R. Hanson, Steven Jacques

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

The development of atherosclerotic plaques includes changes in the cellular and extracellular composition of the arterial wall. Although these changes in composition affect the manner in which light scatters in the vessel wall and thus affect any optical signal, experimentally determining how features of atherosclerosis affect optical signals has remained elusive. Using current tissue-engineering methods, we developed a 3D tissue construct model for assessing how certain features of atherosclerosis (the increased concentrations of lipids and macrophages) affect optical signals. The model is based on vascular tissue constructs made of smooth muscle cells (SMCs) and macrophages (MOs) that are co-cultured inside a 3D scaffold matrix of collagen fibers with interspersed lipids. To make the scaffold matrix, powdered collagen was dissolved in acetic acid, homogenized, and neutralized by sequential dialyses to yield a soft gel of 2 um thick collagen fibers in which cells were seeded. In "normal" constructs, only SMCs were seeded in the collagen gel; in "athero-like" constructs, both SMCs and MΦs (loaded or unloaded with lipid) were seeded in the gel. To demonstrate the use of this model, sets of slab-shaped normal and athero-like constructs were imaged by optical coherence tomography (OCT) and qualitatively analyzed. 2D frames from 3D OCT image cubes were compared to 2D histology sections. Our results indicate that the cellular composition of the construct affects morphological features of the OCT image.

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6439
DOIs
StatePublished - 2007
EventOptics in Tissue Engineering and Regenerative Medicine - San Jose, CA, United States
Duration: Jan 21 2007Jan 23 2007

Other

OtherOptics in Tissue Engineering and Regenerative Medicine
CountryUnited States
CitySan Jose, CA
Period1/21/071/23/07

Fingerprint

Collagen
Light scattering
Optical tomography
Tissue
Lipids
Muscle
Gels
Macrophages
Scaffolds (biology)
Chemical analysis
Histology
Dialysis
Fibers
Tissue engineering
Acetic acid
Cells

Keywords

  • Collagen gel matrix
  • Optical coherence tomography
  • Tissue engineering
  • Tissue phantoms
  • Vascular imaging

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Levitz, D., Hinds, M., Wang, R. K., Zhenhe, M., Ishii, K., Tran, N., ... Jacques, S. (2007). A tissue-engineered 3D model of light scattering in atherosclerotic plaques. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 6439). [643905] https://doi.org/10.1117/12.701313

A tissue-engineered 3D model of light scattering in atherosclerotic plaques. / Levitz, David; Hinds, Monica; Wang, Ruikang K.; Zhenhe, Ma; Ishii, Katsu; Tran, Noi; McCarty, Owen; Hanson, Stephen R.; Jacques, Steven.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6439 2007. 643905.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Levitz, D, Hinds, M, Wang, RK, Zhenhe, M, Ishii, K, Tran, N, McCarty, O, Hanson, SR & Jacques, S 2007, A tissue-engineered 3D model of light scattering in atherosclerotic plaques. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 6439, 643905, Optics in Tissue Engineering and Regenerative Medicine, San Jose, CA, United States, 1/21/07. https://doi.org/10.1117/12.701313
Levitz D, Hinds M, Wang RK, Zhenhe M, Ishii K, Tran N et al. A tissue-engineered 3D model of light scattering in atherosclerotic plaques. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6439. 2007. 643905 https://doi.org/10.1117/12.701313
Levitz, David ; Hinds, Monica ; Wang, Ruikang K. ; Zhenhe, Ma ; Ishii, Katsu ; Tran, Noi ; McCarty, Owen ; Hanson, Stephen R. ; Jacques, Steven. / A tissue-engineered 3D model of light scattering in atherosclerotic plaques. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6439 2007.
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