A TCR Vα CDR3-specific motif associated with lewis rat autoimmune encephalomyelitis and basic protein-specific T cell clones

Abigail C. Buenafe, Rachel C. Tsu, Bruce Bebo, Antony C. Bakke, Arthur A. Vandenbark, Halina Offner

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

To investigate TCR Vα gene expression in the Lewis rat model of experimental autoimmune encephalomyelitis, we obtained Vα chain sequences from two Vβ8.2-encephalitogenic, BP72-89-specific T cell clones. Two different Vα genes, a Vα2 gene and a Vα23 gene, are utilized, but both were found to contain an asparagine repeat (Asn3+) sequence present in the Vα CDR3 region. This Asn3+ motif is also present in the previously reported sequence of a BP68-88-specific hybridoma, 510, which utilizes a different Vα2 gene family member. In further experiments, spinal cord T cells were isolated at the onset of basic protein (BP)-induced disease and sorted for the OX-40 activation marker, which we have previously used to enrich for specifically activated T cells. Analysis of Vα expression in the OX-40+ population revealed the biased use of three Vα genes, Vα1, Vα2, and Vα23. The Asn3+ motif was present in the Vα CDR3 region of Vα1, Vα2, and Vα23 cDNA derived from OX-40+ spinal cord T cells but found to be generally absent in the OX-40- spinal cord population. Since these Asn3+ motif-bearing Vα chain sequences are nearly identical to those utilized by the BP-specific encephalitogenic clones described, it is likely that these Vα sequences are derived from disease-associated T cells in the spinal cord. Thus, we demonstrate that the Asn3+ Vα CDR3 motif is strongly associated with experimental autoimmune encephalomyelitis in the Lewis rat and propose that it plays a role in TCR recognition of a specific BP peptide/MHC complex.

Original languageEnglish (US)
Pages (from-to)5472-5483
Number of pages12
JournalJournal of Immunology
Volume158
Issue number11
StatePublished - Dec 1 1997

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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