TY - JOUR
T1 - A SWI/SNF-related chromatin remodeling complex, E-RC1, is required for tissue-specific transcriptional regulation by EKLF in vitro
AU - Armstrong, Jennifer A.
AU - Bieker, James J.
AU - Emerson, Beverly M.
N1 - Funding Information:
We thank Drs. Gerald Crabtree and Keji Zhao for their generous gift of BAF antibodies; Dr. Weidong Wang for helpful discussions and advice as well as purified Jurkat T cell SWI/SNF and BAF57; Drs. Jerry Workman and David Steger for the 5S positioning sequence plasmid; and Drs. Katherine Jones and Glenn McAlpine for their help in analyzing E-RC1 with TFE-3 and HIV-1. We are indebted to Drs. Gerald Crabtree, Katherine Jones, Lori Westin, and Joseph Schulz for helpful discussions and a critical reading of this manuscript. This work was supported by Grant GM 38760 from the National Institutes of Health (B. M. E.) and a scholarship from the Leukemia Society of America (J. J. B.).
PY - 1998/10/2
Y1 - 1998/10/2
N2 - Erythroid Kruppel-like factor (EKLF) is necessary for stage-specific expression of the human [β-globin gene. We show that EKLF requires a SWI/SNF-related chromatin remodeling complex, EKLF coactivator-remodeling complex 1 (E-RC1), to generate a DNase I hypersensitive, transcriptionally active β-globin promoter on chromatin templates in vitro. E-RC1 contains BRG1, BAF170, BAF155, and INI1 {BAF47) homologs of yeast SWI/SNF subunits, as well as a subunit unique to higher eukaryotes, BAF57, which is critical for chromatin remodeling and transcription with EKLF. E-RC1 displays functional selectivity toward transcription factors, since it cannot activate expression of chromatin-assembled HIV-1 templates with the E box-binding protein TFE-3. Thus, a member of the SWI/SNF family acts directly in transcriptional activation and may regulate subsets of genes by selectively interacting with specific DNA-binding proteins.
AB - Erythroid Kruppel-like factor (EKLF) is necessary for stage-specific expression of the human [β-globin gene. We show that EKLF requires a SWI/SNF-related chromatin remodeling complex, EKLF coactivator-remodeling complex 1 (E-RC1), to generate a DNase I hypersensitive, transcriptionally active β-globin promoter on chromatin templates in vitro. E-RC1 contains BRG1, BAF170, BAF155, and INI1 {BAF47) homologs of yeast SWI/SNF subunits, as well as a subunit unique to higher eukaryotes, BAF57, which is critical for chromatin remodeling and transcription with EKLF. E-RC1 displays functional selectivity toward transcription factors, since it cannot activate expression of chromatin-assembled HIV-1 templates with the E box-binding protein TFE-3. Thus, a member of the SWI/SNF family acts directly in transcriptional activation and may regulate subsets of genes by selectively interacting with specific DNA-binding proteins.
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U2 - 10.1016/S0092-8674(00)81785-7
DO - 10.1016/S0092-8674(00)81785-7
M3 - Article
C2 - 9778250
AN - SCOPUS:0032475845
SN - 0092-8674
VL - 95
SP - 93
EP - 104
JO - Cell
JF - Cell
IS - 1
ER -