A SWI/SNF-related chromatin remodeling complex, E-RC1, is required for tissue-specific transcriptional regulation by EKLF in vitro

Jennifer A. Armstrong, James J. Bieker, Beverly M. Emerson

Research output: Contribution to journalArticle

260 Scopus citations

Abstract

Erythroid Kruppel-like factor (EKLF) is necessary for stage-specific expression of the human [β-globin gene. We show that EKLF requires a SWI/SNF-related chromatin remodeling complex, EKLF coactivator-remodeling complex 1 (E-RC1), to generate a DNase I hypersensitive, transcriptionally active β-globin promoter on chromatin templates in vitro. E-RC1 contains BRG1, BAF170, BAF155, and INI1 {BAF47) homologs of yeast SWI/SNF subunits, as well as a subunit unique to higher eukaryotes, BAF57, which is critical for chromatin remodeling and transcription with EKLF. E-RC1 displays functional selectivity toward transcription factors, since it cannot activate expression of chromatin-assembled HIV-1 templates with the E box-binding protein TFE-3. Thus, a member of the SWI/SNF family acts directly in transcriptional activation and may regulate subsets of genes by selectively interacting with specific DNA-binding proteins.

Original languageEnglish (US)
Pages (from-to)93-104
Number of pages12
JournalCell
Volume95
Issue number1
DOIs
StatePublished - Oct 2 1998

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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