A subpopulation of olfactory bulb GABAergic interneurons is derived from Emx1- and Dlx5/6-expressing progenitors

Minoree Kohwi, Magdalena A. Petryniak, Jason E. Long, Marc Ekker, Kunihiko Obata, Yuchio Yanagawa, John L.R. Rubenstein, Arturo Alvarez-Buylla

Research output: Contribution to journalArticle

175 Scopus citations

Abstract

The subventricular zone (SVZ) of the postnatal brain continuously generates olfactory bulb (OB) interneurons. We show that calretinin+, calbindin+, and dopaminergic (TH+) periglomerular OB interneurons correspond to distinct subtypes of GABAergic cells; all were produced in the postnatal mouse brain, but they matured and were eliminated at different rates. The embryonic lateral ganglionic eminence (LGE) is thought to be the site of origin of postnatal SVZ neural progenitors. Consistently, grafts of the embryonic LGE into the adult brain SVZ generated many OB interneurons, including TH+ and calbindin+ periglomerular interneurons. However, calretinin+ cells were not produced from these LGE grafts. Surprisingly, pallial and septal embryonic progenitors transplanted into the adult brain SVZ also resulted in the generation of OB interneurons, including calretinin+ cells. A subset of Dlx2+ OB interneurons was derived from cells expressingEmx1, a transcription factor largely restricted to the pallium during development. Emx1 lineage-derived cells contributed a substantial portion of GABAergic cells in the OB, including calretinin + interneurons. This is in contrast to cortex, in which Emx1 lineage-derived cells do not differentiate into GABAergic neurons. Our results suggest that some OB interneurons are derived from progenitors outside the LGE and that precursors expressing what has classically been considered a pallial transcription factor generate GABAergic interneurons.

Original languageEnglish (US)
Pages (from-to)6878-6891
Number of pages14
JournalJournal of Neuroscience
Volume27
Issue number26
DOIs
StatePublished - Jun 27 2007

Keywords

  • Dlx2
  • Emx1
  • GABAergic neuron
  • Neurogenesis
  • Olfactory bulb
  • Pallial
  • SVZ

ASJC Scopus subject areas

  • Neuroscience(all)

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    Kohwi, M., Petryniak, M. A., Long, J. E., Ekker, M., Obata, K., Yanagawa, Y., Rubenstein, J. L. R., & Alvarez-Buylla, A. (2007). A subpopulation of olfactory bulb GABAergic interneurons is derived from Emx1- and Dlx5/6-expressing progenitors. Journal of Neuroscience, 27(26), 6878-6891. https://doi.org/10.1523/JNEUROSCI.0254-07.2007