TY - JOUR
T1 - A subpopulation of olfactory bulb GABAergic interneurons is derived from Emx1- and Dlx5/6-expressing progenitors
AU - Kohwi, Minoree
AU - Petryniak, Magdalena A.
AU - Long, Jason E.
AU - Ekker, Marc
AU - Obata, Kunihiko
AU - Yanagawa, Yuchio
AU - Rubenstein, John L.R.
AU - Alvarez-Buylla, Arturo
PY - 2007/6/27
Y1 - 2007/6/27
N2 - The subventricular zone (SVZ) of the postnatal brain continuously generates olfactory bulb (OB) interneurons. We show that calretinin+, calbindin+, and dopaminergic (TH+) periglomerular OB interneurons correspond to distinct subtypes of GABAergic cells; all were produced in the postnatal mouse brain, but they matured and were eliminated at different rates. The embryonic lateral ganglionic eminence (LGE) is thought to be the site of origin of postnatal SVZ neural progenitors. Consistently, grafts of the embryonic LGE into the adult brain SVZ generated many OB interneurons, including TH+ and calbindin+ periglomerular interneurons. However, calretinin+ cells were not produced from these LGE grafts. Surprisingly, pallial and septal embryonic progenitors transplanted into the adult brain SVZ also resulted in the generation of OB interneurons, including calretinin+ cells. A subset of Dlx2+ OB interneurons was derived from cells expressingEmx1, a transcription factor largely restricted to the pallium during development. Emx1 lineage-derived cells contributed a substantial portion of GABAergic cells in the OB, including calretinin + interneurons. This is in contrast to cortex, in which Emx1 lineage-derived cells do not differentiate into GABAergic neurons. Our results suggest that some OB interneurons are derived from progenitors outside the LGE and that precursors expressing what has classically been considered a pallial transcription factor generate GABAergic interneurons.
AB - The subventricular zone (SVZ) of the postnatal brain continuously generates olfactory bulb (OB) interneurons. We show that calretinin+, calbindin+, and dopaminergic (TH+) periglomerular OB interneurons correspond to distinct subtypes of GABAergic cells; all were produced in the postnatal mouse brain, but they matured and were eliminated at different rates. The embryonic lateral ganglionic eminence (LGE) is thought to be the site of origin of postnatal SVZ neural progenitors. Consistently, grafts of the embryonic LGE into the adult brain SVZ generated many OB interneurons, including TH+ and calbindin+ periglomerular interneurons. However, calretinin+ cells were not produced from these LGE grafts. Surprisingly, pallial and septal embryonic progenitors transplanted into the adult brain SVZ also resulted in the generation of OB interneurons, including calretinin+ cells. A subset of Dlx2+ OB interneurons was derived from cells expressingEmx1, a transcription factor largely restricted to the pallium during development. Emx1 lineage-derived cells contributed a substantial portion of GABAergic cells in the OB, including calretinin + interneurons. This is in contrast to cortex, in which Emx1 lineage-derived cells do not differentiate into GABAergic neurons. Our results suggest that some OB interneurons are derived from progenitors outside the LGE and that precursors expressing what has classically been considered a pallial transcription factor generate GABAergic interneurons.
KW - Dlx2
KW - Emx1
KW - GABAergic neuron
KW - Neurogenesis
KW - Olfactory bulb
KW - Pallial
KW - SVZ
UR - http://www.scopus.com/inward/record.url?scp=34347337683&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34347337683&partnerID=8YFLogxK
U2 - 10.1523/JNEUROSCI.0254-07.2007
DO - 10.1523/JNEUROSCI.0254-07.2007
M3 - Article
C2 - 17596436
AN - SCOPUS:34347337683
SN - 0270-6474
VL - 27
SP - 6878
EP - 6891
JO - Journal of Neuroscience
JF - Journal of Neuroscience
IS - 26
ER -