A structural determinant of differential sensitivity of cloned inward rectifier K+ channels to intracellular spermine

B. Fakler, U. Brändle, Ch Bond, E. Glowatzki, C. König, John Adelman, H. P. Zenner, J. P. Ruppersberg

Research output: Contribution to journalArticle

125 Citations (Scopus)

Abstract

Large subtype-specific differences in the sensitivity of cloned inward-rectifier K+ channels of the IRK1, BIR10 and ROMK1 subtype to being blocked by intracellular spermine (SPM) are described. It is shown, by site-directed mutagenesis, that the four orders of magnitude larger SPM sensitivity of BIR10 channels compared to ROMK1 channels may be explained by a difference in a single amino acid in the putative transmembrane segment TMII. This residue, a negatively charged glutamate in BIR10, is homologous to the residue in IRK1 and ROMK1 which has previously been shown to change gating properties and Mg2+ sensitivity. Differential block by physiological SPM concentrations is suggested as a major functional difference between subtypes of inward-rectifier K+ channels.

Original languageEnglish (US)
Pages (from-to)199-203
Number of pages5
JournalFEBS Letters
Volume356
Issue number2-3
DOIs
StatePublished - Dec 19 1994

Fingerprint

Inwardly Rectifying Potassium Channel
Spermine
Mutagenesis
Site-Directed Mutagenesis
Glutamic Acid
Amino Acids

Keywords

  • Clone
  • Inward rectifier
  • K channel
  • Site-directed mutagenesis
  • Spermine

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Fakler, B., Brändle, U., Bond, C., Glowatzki, E., König, C., Adelman, J., ... Ruppersberg, J. P. (1994). A structural determinant of differential sensitivity of cloned inward rectifier K+ channels to intracellular spermine. FEBS Letters, 356(2-3), 199-203. https://doi.org/10.1016/0014-5793(94)01258-X

A structural determinant of differential sensitivity of cloned inward rectifier K+ channels to intracellular spermine. / Fakler, B.; Brändle, U.; Bond, Ch; Glowatzki, E.; König, C.; Adelman, John; Zenner, H. P.; Ruppersberg, J. P.

In: FEBS Letters, Vol. 356, No. 2-3, 19.12.1994, p. 199-203.

Research output: Contribution to journalArticle

Fakler, B, Brändle, U, Bond, C, Glowatzki, E, König, C, Adelman, J, Zenner, HP & Ruppersberg, JP 1994, 'A structural determinant of differential sensitivity of cloned inward rectifier K+ channels to intracellular spermine', FEBS Letters, vol. 356, no. 2-3, pp. 199-203. https://doi.org/10.1016/0014-5793(94)01258-X
Fakler, B. ; Brändle, U. ; Bond, Ch ; Glowatzki, E. ; König, C. ; Adelman, John ; Zenner, H. P. ; Ruppersberg, J. P. / A structural determinant of differential sensitivity of cloned inward rectifier K+ channels to intracellular spermine. In: FEBS Letters. 1994 ; Vol. 356, No. 2-3. pp. 199-203.
@article{8fa1a9de6cb6474c913e4097777144a6,
title = "A structural determinant of differential sensitivity of cloned inward rectifier K+ channels to intracellular spermine",
abstract = "Large subtype-specific differences in the sensitivity of cloned inward-rectifier K+ channels of the IRK1, BIR10 and ROMK1 subtype to being blocked by intracellular spermine (SPM) are described. It is shown, by site-directed mutagenesis, that the four orders of magnitude larger SPM sensitivity of BIR10 channels compared to ROMK1 channels may be explained by a difference in a single amino acid in the putative transmembrane segment TMII. This residue, a negatively charged glutamate in BIR10, is homologous to the residue in IRK1 and ROMK1 which has previously been shown to change gating properties and Mg2+ sensitivity. Differential block by physiological SPM concentrations is suggested as a major functional difference between subtypes of inward-rectifier K+ channels.",
keywords = "Clone, Inward rectifier, K channel, Site-directed mutagenesis, Spermine",
author = "B. Fakler and U. Br{\"a}ndle and Ch Bond and E. Glowatzki and C. K{\"o}nig and John Adelman and Zenner, {H. P.} and Ruppersberg, {J. P.}",
year = "1994",
month = "12",
day = "19",
doi = "10.1016/0014-5793(94)01258-X",
language = "English (US)",
volume = "356",
pages = "199--203",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "2-3",

}

TY - JOUR

T1 - A structural determinant of differential sensitivity of cloned inward rectifier K+ channels to intracellular spermine

AU - Fakler, B.

AU - Brändle, U.

AU - Bond, Ch

AU - Glowatzki, E.

AU - König, C.

AU - Adelman, John

AU - Zenner, H. P.

AU - Ruppersberg, J. P.

PY - 1994/12/19

Y1 - 1994/12/19

N2 - Large subtype-specific differences in the sensitivity of cloned inward-rectifier K+ channels of the IRK1, BIR10 and ROMK1 subtype to being blocked by intracellular spermine (SPM) are described. It is shown, by site-directed mutagenesis, that the four orders of magnitude larger SPM sensitivity of BIR10 channels compared to ROMK1 channels may be explained by a difference in a single amino acid in the putative transmembrane segment TMII. This residue, a negatively charged glutamate in BIR10, is homologous to the residue in IRK1 and ROMK1 which has previously been shown to change gating properties and Mg2+ sensitivity. Differential block by physiological SPM concentrations is suggested as a major functional difference between subtypes of inward-rectifier K+ channels.

AB - Large subtype-specific differences in the sensitivity of cloned inward-rectifier K+ channels of the IRK1, BIR10 and ROMK1 subtype to being blocked by intracellular spermine (SPM) are described. It is shown, by site-directed mutagenesis, that the four orders of magnitude larger SPM sensitivity of BIR10 channels compared to ROMK1 channels may be explained by a difference in a single amino acid in the putative transmembrane segment TMII. This residue, a negatively charged glutamate in BIR10, is homologous to the residue in IRK1 and ROMK1 which has previously been shown to change gating properties and Mg2+ sensitivity. Differential block by physiological SPM concentrations is suggested as a major functional difference between subtypes of inward-rectifier K+ channels.

KW - Clone

KW - Inward rectifier

KW - K channel

KW - Site-directed mutagenesis

KW - Spermine

UR - http://www.scopus.com/inward/record.url?scp=0028028412&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028028412&partnerID=8YFLogxK

U2 - 10.1016/0014-5793(94)01258-X

DO - 10.1016/0014-5793(94)01258-X

M3 - Article

C2 - 7805837

AN - SCOPUS:0028028412

VL - 356

SP - 199

EP - 203

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 2-3

ER -