TY - JOUR
T1 - A standardized plaque reduction assay for determination of drug susceptibilities of cytomegalovirus clinical isolates
AU - Landry, Marie L.
AU - Stanat, Sylvia
AU - Biron, Karen
AU - Brambilla, Donald
AU - Britt, William
AU - Jokela, Janet
AU - Chou, Sunwen
AU - Drew, W. Lawrence
AU - Erice, Alejo
AU - Gilliam, Bruce
AU - Lurain, Nell
AU - Manischewitz, Jody
AU - Miner, Richard
AU - Nokta, Mostafa
AU - Reichelderfer, Patricia
AU - Spector, Stephen
AU - Weinberg, Adriana
AU - Yen-Lieberman, Belinda
AU - Crumpacker, Clyde
PY - 2000/3
Y1 - 2000/3
N2 - Twelve laboratories collaborated in formulating and testing a standardized plaque reduction assay for cytomegalovirus (CMV) cell-associated clinical isolates. Four characterized and plaque-purified CMV strains, as well as six coded clinical isolates obtained after antiviral therapy, were distributed and tested. Good agreement was obtained for four of the clinical isolates, but a broad distribution of results was obtained for two isolates. Analysis of these results indicates the problems associated with clinical isolates, including the large genetic variability and the highly cell- associated phenotype. This collaborative effort, by addressing these problems, represents a significant step toward the development of a standardized assay.
AB - Twelve laboratories collaborated in formulating and testing a standardized plaque reduction assay for cytomegalovirus (CMV) cell-associated clinical isolates. Four characterized and plaque-purified CMV strains, as well as six coded clinical isolates obtained after antiviral therapy, were distributed and tested. Good agreement was obtained for four of the clinical isolates, but a broad distribution of results was obtained for two isolates. Analysis of these results indicates the problems associated with clinical isolates, including the large genetic variability and the highly cell- associated phenotype. This collaborative effort, by addressing these problems, represents a significant step toward the development of a standardized assay.
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U2 - 10.1128/AAC.44.3.688-692.2000
DO - 10.1128/AAC.44.3.688-692.2000
M3 - Article
C2 - 10681339
AN - SCOPUS:0033996648
SN - 0066-4804
VL - 44
SP - 688
EP - 692
JO - Antimicrobial agents and chemotherapy
JF - Antimicrobial agents and chemotherapy
IS - 3
ER -