Abstract
Current methods for placental tissue collection assess a delivered organ without direct functional correlates; therefore, the four-quadrant biopsy protocol utilized by many researchers may provide reasonable representation of tissue across a large organ, and offer a snapshot for molecular analysis of the placenta. However, the recent impetus to understand the placenta in real time, and the use of functional imaging to comprehend placental biology, warrants a different sampling approach. Here we present a method to standardize placental tissue collection in a format designed to facilitate correlation of in vivo function with ex vivo assessments. Additionally, we draw comparisons to the quadrant biopsy regimen, and highlight a pathological case of placental infarction detected by in utero imaging.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 45-49 |
| Number of pages | 5 |
| Journal | BioTechniques |
| Volume | 67 |
| Issue number | 2 |
| DOIs | |
| State | Published - Jan 1 2019 |
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Keywords
- Biopsy
- Placenta
- Standardized sampling
ASJC Scopus subject areas
- Biotechnology
- Biochemistry, Genetics and Molecular Biology(all)
Cite this
A standardized method for collection of human placenta samples in the age of functional magnetic resonance imaging. / Roberts, Victoria; Gaffney, Jessica E.; Lewandowski, Katherine S.; Schabel, Matthias; Morgan, Terry; Frias, Antonio.
In: BioTechniques, Vol. 67, No. 2, 01.01.2019, p. 45-49.Research output: Contribution to journal › Article
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TY - JOUR
T1 - A standardized method for collection of human placenta samples in the age of functional magnetic resonance imaging
AU - Roberts, Victoria
AU - Gaffney, Jessica E.
AU - Lewandowski, Katherine S.
AU - Schabel, Matthias
AU - Morgan, Terry
AU - Frias, Antonio
PY - 2019/1/1
Y1 - 2019/1/1
N2 - Current methods for placental tissue collection assess a delivered organ without direct functional correlates; therefore, the four-quadrant biopsy protocol utilized by many researchers may provide reasonable representation of tissue across a large organ, and offer a snapshot for molecular analysis of the placenta. However, the recent impetus to understand the placenta in real time, and the use of functional imaging to comprehend placental biology, warrants a different sampling approach. Here we present a method to standardize placental tissue collection in a format designed to facilitate correlation of in vivo function with ex vivo assessments. Additionally, we draw comparisons to the quadrant biopsy regimen, and highlight a pathological case of placental infarction detected by in utero imaging.
AB - Current methods for placental tissue collection assess a delivered organ without direct functional correlates; therefore, the four-quadrant biopsy protocol utilized by many researchers may provide reasonable representation of tissue across a large organ, and offer a snapshot for molecular analysis of the placenta. However, the recent impetus to understand the placenta in real time, and the use of functional imaging to comprehend placental biology, warrants a different sampling approach. Here we present a method to standardize placental tissue collection in a format designed to facilitate correlation of in vivo function with ex vivo assessments. Additionally, we draw comparisons to the quadrant biopsy regimen, and highlight a pathological case of placental infarction detected by in utero imaging.
KW - Biopsy
KW - Placenta
KW - Standardized sampling
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U2 - 10.2144/btn-2019-0029
DO - 10.2144/btn-2019-0029
M3 - Article
VL - 67
SP - 45
EP - 49
JO - BioTechniques
JF - BioTechniques
SN - 0736-6205
IS - 2
ER -