A standardized method for collection of human placenta samples in the age of functional magnetic resonance imaging

Victoria Roberts; Jessica E. Gaffney; Katherine S. Lewandowski; Matthias C. Schabel; Terry K. Morgan; Antonio E. Frias

doi:10.2144/btn-2019-0029

A standardized method for collection of human placenta samples in the age of functional magnetic resonance imaging

Victoria Roberts, Jessica E. Gaffney, Katherine S. Lewandowski, Matthias C. Schabel, Terry K. Morgan, Antonio E. Frias

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Current methods for placental tissue collection assess a delivered organ without direct functional correlates; therefore, the four-quadrant biopsy protocol utilized by many researchers may provide reasonable representation of tissue across a large organ, and offer a snapshot for molecular analysis of the placenta. However, the recent impetus to understand the placenta in real time, and the use of functional imaging to comprehend placental biology, warrants a different sampling approach. Here we present a method to standardize placental tissue collection in a format designed to facilitate correlation of in vivo function with ex vivo assessments. Additionally, we draw comparisons to the quadrant biopsy regimen, and highlight a pathological case of placental infarction detected by in utero imaging.

Original language	English (US)
Pages (from-to)	45-49
Number of pages	5
Journal	BioTechniques
Volume	67
Issue number	2
DOIs	https://doi.org/10.2144/btn-2019-0029
State	Published - 2019

Keywords

Biopsy
Placenta
Standardized sampling

ASJC Scopus subject areas

Biotechnology
Biochemistry, Genetics and Molecular Biology(all)

Access to Document

10.2144/btn-2019-0029

Cite this

@article{4b302f50b7074711a3d0cfc84556cd84,

title = "A standardized method for collection of human placenta samples in the age of functional magnetic resonance imaging",

abstract = "Current methods for placental tissue collection assess a delivered organ without direct functional correlates; therefore, the four-quadrant biopsy protocol utilized by many researchers may provide reasonable representation of tissue across a large organ, and offer a snapshot for molecular analysis of the placenta. However, the recent impetus to understand the placenta in real time, and the use of functional imaging to comprehend placental biology, warrants a different sampling approach. Here we present a method to standardize placental tissue collection in a format designed to facilitate correlation of in vivo function with ex vivo assessments. Additionally, we draw comparisons to the quadrant biopsy regimen, and highlight a pathological case of placental infarction detected by in utero imaging.",

keywords = "Biopsy, Placenta, Standardized sampling",

author = "Victoria Roberts and Gaffney, {Jessica E.} and Lewandowski, {Katherine S.} and Schabel, {Matthias C.} and Morgan, {Terry K.} and Frias, {Antonio E.}",

year = "2019",

doi = "10.2144/btn-2019-0029",

language = "English (US)",

volume = "67",

pages = "45--49",

journal = "BioTechniques",

issn = "0736-6205",

publisher = "Eaton Publishing Company",

number = "2",

}

TY - JOUR

T1 - A standardized method for collection of human placenta samples in the age of functional magnetic resonance imaging

AU - Roberts, Victoria

AU - Gaffney, Jessica E.

AU - Lewandowski, Katherine S.

AU - Schabel, Matthias C.

AU - Morgan, Terry K.

AU - Frias, Antonio E.

PY - 2019

Y1 - 2019

N2 - Current methods for placental tissue collection assess a delivered organ without direct functional correlates; therefore, the four-quadrant biopsy protocol utilized by many researchers may provide reasonable representation of tissue across a large organ, and offer a snapshot for molecular analysis of the placenta. However, the recent impetus to understand the placenta in real time, and the use of functional imaging to comprehend placental biology, warrants a different sampling approach. Here we present a method to standardize placental tissue collection in a format designed to facilitate correlation of in vivo function with ex vivo assessments. Additionally, we draw comparisons to the quadrant biopsy regimen, and highlight a pathological case of placental infarction detected by in utero imaging.

AB - Current methods for placental tissue collection assess a delivered organ without direct functional correlates; therefore, the four-quadrant biopsy protocol utilized by many researchers may provide reasonable representation of tissue across a large organ, and offer a snapshot for molecular analysis of the placenta. However, the recent impetus to understand the placenta in real time, and the use of functional imaging to comprehend placental biology, warrants a different sampling approach. Here we present a method to standardize placental tissue collection in a format designed to facilitate correlation of in vivo function with ex vivo assessments. Additionally, we draw comparisons to the quadrant biopsy regimen, and highlight a pathological case of placental infarction detected by in utero imaging.

KW - Biopsy

KW - Placenta

KW - Standardized sampling

UR - http://www.scopus.com/inward/record.url?scp=85070116016&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85070116016&partnerID=8YFLogxK

U2 - 10.2144/btn-2019-0029

DO - 10.2144/btn-2019-0029

M3 - Article

C2 - 31184493

AN - SCOPUS:85070116016

SN - 0736-6205

VL - 67

SP - 45

EP - 49

JO - BioTechniques

JF - BioTechniques

IS - 2

ER -

A standardized method for collection of human placenta samples in the age of functional magnetic resonance imaging

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this