A standardized method for collection of human placenta samples in the age of functional magnetic resonance imaging

Victoria H.J. Roberts; Jessica E. Gaffney; Katherine S. Lewandowski; Matthias C. Schabel; Terry K. Morgan; Antonio E. Frias

doi:10.2144/btn-2019-0029

A standardized method for collection of human placenta samples in the age of functional magnetic resonance imaging

Victoria H.J. Roberts, Jessica E. Gaffney, Katherine S. Lewandowski, Matthias C. Schabel, Terry K. Morgan, Antonio E. Frias

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Current methods for placental tissue collection assess a delivered organ without direct functional correlates; therefore, the four-quadrant biopsy protocol utilized by many researchers may provide reasonable representation of tissue across a large organ, and offer a snapshot for molecular analysis of the placenta. However, the recent impetus to understand the placenta in real time, and the use of functional imaging to comprehend placental biology, warrants a different sampling approach. Here we present a method to standardize placental tissue collection in a format designed to facilitate correlation of in vivo function with ex vivo assessments. Additionally, we draw comparisons to the quadrant biopsy regimen, and highlight a pathological case of placental infarction detected by in utero imaging.

Original language	English (US)
Pages (from-to)	45-49
Number of pages	5
Journal	BioTechniques
Volume	67
Issue number	2
DOIs	https://doi.org/10.2144/btn-2019-0029
State	Published - 2019

Keywords

Biopsy
Placenta
Standardized sampling

ASJC Scopus subject areas

Biotechnology
Biochemistry, Genetics and Molecular Biology(all)

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abstract = "Current methods for placental tissue collection assess a delivered organ without direct functional correlates; therefore, the four-quadrant biopsy protocol utilized by many researchers may provide reasonable representation of tissue across a large organ, and offer a snapshot for molecular analysis of the placenta. However, the recent impetus to understand the placenta in real time, and the use of functional imaging to comprehend placental biology, warrants a different sampling approach. Here we present a method to standardize placental tissue collection in a format designed to facilitate correlation of in vivo function with ex vivo assessments. Additionally, we draw comparisons to the quadrant biopsy regimen, and highlight a pathological case of placental infarction detected by in utero imaging.",

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AU - Morgan, Terry K.

AU - Frias, Antonio E.

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