A stable isotope biomarker of marine food intake captures associations between n-3 fatty acid intake and chronic disease risk in a Yup'ik study population, and detects new associations with blood pressure and adiponectin

Diane M. O'Brien, Alan R. Kristal, Sarah H. Nash, Scarlett E. Hopkins, Bret R. Luick, Kimber L. Stanhope, Peter J. Havel, Bert Boyer

Research output: Contribution to journalArticle

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Abstract

The nitrogen isotope ratio (δ15N) of RBCs has been proposed as a biomarker of marine food intake in Yup'ik people based on strong associations with RBC eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). However, EPA and DHA derive from marine fats, whereas elevated δ15N derives from marine protein, and these dietary components may have different biologic effects. Whether δ15N is similarly associated with chronic disease risk factors compared with RBC EPA and DHA is not known. We used covariate-adjusted linear models to describe biomarker associations with chronic disease risk factors in Yup'ik people, first in a smaller (n = 363) cross-sectional study population using RBC EPA, DHA, and δ15N, and then in a larger (n = 772) cross-sectional study population using δ15N only. In the smaller sample, associations of RBC EPA, DHA, and δ15N with obesity and chronic disease risk factors were similar in direction and significance: δ15N was positively associated with total, HDL, and LDL cholesterol, apolipoprotein A-I, and insulin-like growth factor binding protein-3 (IGFBP-3), and inversely associated with triglycerides. Based on comparisons between covariate-adjusted b-coefficients, EPA was more strongly associated with circulating lipids and lipoproteins, whereas δ15N was more strongly associated with adipokines, the inflammatory marker interleukin-6, and IGFBP-3. In the larger sample there were new findings for this population: δ15N was inversely associated with blood pressure and there was a significant association (with inverse linear and positive quadratic terms) with adiponectin. In conclusion, δ15N is a valid measure for evaluating associations between EPA and DHA intake and chronic disease risk in Yup'ik people and may be used in larger studies. By measuring δ15N, we report beneficial associations of marine food intake with blood pressure and adiponectin, which may contribute to a lower incidence of some chronic diseases in Yup'ik people.

Original languageEnglish (US)
Pages (from-to)706-713
Number of pages8
JournalJournal of Nutrition
Volume144
Issue number5
DOIs
StatePublished - Jan 1 2014
Externally publishedYes

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Eicosapentaenoic Acid
Adiponectin
Omega-3 Fatty Acids
Docosahexaenoic Acids
Isotopes
Chronic Disease
Eating
Biomarkers
Blood Pressure
Population
Insulin-Like Growth Factor Binding Protein 3
Nitrogen Isotopes
Cross-Sectional Studies
Adipokines
Dietary Proteins
Apolipoprotein A-I
LDL Cholesterol
HDL Cholesterol
Lipoproteins
Linear Models

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

A stable isotope biomarker of marine food intake captures associations between n-3 fatty acid intake and chronic disease risk in a Yup'ik study population, and detects new associations with blood pressure and adiponectin. / O'Brien, Diane M.; Kristal, Alan R.; Nash, Sarah H.; Hopkins, Scarlett E.; Luick, Bret R.; Stanhope, Kimber L.; Havel, Peter J.; Boyer, Bert.

In: Journal of Nutrition, Vol. 144, No. 5, 01.01.2014, p. 706-713.

Research output: Contribution to journalArticle

O'Brien, Diane M. ; Kristal, Alan R. ; Nash, Sarah H. ; Hopkins, Scarlett E. ; Luick, Bret R. ; Stanhope, Kimber L. ; Havel, Peter J. ; Boyer, Bert. / A stable isotope biomarker of marine food intake captures associations between n-3 fatty acid intake and chronic disease risk in a Yup'ik study population, and detects new associations with blood pressure and adiponectin. In: Journal of Nutrition. 2014 ; Vol. 144, No. 5. pp. 706-713.
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AU - Nash, Sarah H.

AU - Hopkins, Scarlett E.

AU - Luick, Bret R.

AU - Stanhope, Kimber L.

AU - Havel, Peter J.

AU - Boyer, Bert

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