A spontaneous deletion of α-Synuclein is associated with an increase in CB1 mRNA transcript and receptor expression in the hippocampus and amygdala: Effects on alcohol consumption

Alejandro López-Jiménez, Nicole A R Walter, Elena Giné, Ángel Santos, Victor Echeverry-Alzate, Kora Mareen Bühler, Pedro Olmos, Stéphanie Giezendanner, Rosario Moratalla, Lluis Montoliu, Kari Buck, Jose Antonio López-Moreno

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

α-Synuclein (α-syn) protein and endocannabinoid CB1 receptors are primarily located in presynaptic terminals. An association between α-syn and CB1 receptors has recently been established in Parkinson's disease, but it is completely unknown whether there is an association between these two proteins in alcohol addiction. Therefore, we aimed to examine the α-syn mRNA transcript and protein expression levels in the prefrontal cortex, striatum, amygdala and hippocampus. These brain regions are the most frequently implicated in alcohol and other drug addiction. In these studies, we used C57BL/6 mice carrying a spontaneous deletion of the α-syn gene (C57BL/6Snca-/-) and their respective controls (C57BL/6Snca+/+). These animals were monitored for spontaneous alcohol consumption (3-10%) and their response to a hypnotic-sedative dose of alcohol (3 g kg-1) was also assessed. Compared with the C57BL/6Snca+/+ mice, we found that the C57BL/6Snca-/- mice exhibited a higher expression level of the CB1 mRNA transcript and CB1 receptor in the hippocampus and amygdala. Furthermore, C57BL/6Snca-/- mice showed an increase in alcohol consumption when offered a 10% alcohol solution. There was no significant difference in sleep time after the injection of 3 g/kg alcohol. These results are the first to reveal an association between α-syn and the CB1 receptor in the brain regions that are most frequently implicated in alcohol and other drug addictions.

Original languageEnglish (US)
Pages (from-to)280-289
Number of pages10
JournalSynapse
Volume67
Issue number6
DOIs
StatePublished - Jun 2013

Fingerprint

Synucleins
Cannabinoid Receptor CB1
Amygdala
Alcohol Drinking
Hippocampus
Inbred C57BL Mouse
Alcohols
Messenger RNA
Substance-Related Disorders
Endocannabinoids
Proteins
Gene Deletion
Presynaptic Terminals
Brain
Prefrontal Cortex
Hypnotics and Sedatives
Alcoholism
Parkinson Disease
Sleep
Injections

Keywords

  • Addiction
  • C57BL/6 mice
  • Cannabinoid
  • Genetic expression
  • Spontaneous mutation

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

Cite this

López-Jiménez, A., Walter, N. A. R., Giné, E., Santos, Á., Echeverry-Alzate, V., Bühler, K. M., ... López-Moreno, J. A. (2013). A spontaneous deletion of α-Synuclein is associated with an increase in CB1 mRNA transcript and receptor expression in the hippocampus and amygdala: Effects on alcohol consumption. Synapse, 67(6), 280-289. https://doi.org/10.1002/syn.21639

A spontaneous deletion of α-Synuclein is associated with an increase in CB1 mRNA transcript and receptor expression in the hippocampus and amygdala : Effects on alcohol consumption. / López-Jiménez, Alejandro; Walter, Nicole A R; Giné, Elena; Santos, Ángel; Echeverry-Alzate, Victor; Bühler, Kora Mareen; Olmos, Pedro; Giezendanner, Stéphanie; Moratalla, Rosario; Montoliu, Lluis; Buck, Kari; López-Moreno, Jose Antonio.

In: Synapse, Vol. 67, No. 6, 06.2013, p. 280-289.

Research output: Contribution to journalArticle

López-Jiménez, A, Walter, NAR, Giné, E, Santos, Á, Echeverry-Alzate, V, Bühler, KM, Olmos, P, Giezendanner, S, Moratalla, R, Montoliu, L, Buck, K & López-Moreno, JA 2013, 'A spontaneous deletion of α-Synuclein is associated with an increase in CB1 mRNA transcript and receptor expression in the hippocampus and amygdala: Effects on alcohol consumption', Synapse, vol. 67, no. 6, pp. 280-289. https://doi.org/10.1002/syn.21639
López-Jiménez, Alejandro ; Walter, Nicole A R ; Giné, Elena ; Santos, Ángel ; Echeverry-Alzate, Victor ; Bühler, Kora Mareen ; Olmos, Pedro ; Giezendanner, Stéphanie ; Moratalla, Rosario ; Montoliu, Lluis ; Buck, Kari ; López-Moreno, Jose Antonio. / A spontaneous deletion of α-Synuclein is associated with an increase in CB1 mRNA transcript and receptor expression in the hippocampus and amygdala : Effects on alcohol consumption. In: Synapse. 2013 ; Vol. 67, No. 6. pp. 280-289.
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AU - Giezendanner, Stéphanie

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