A solid phase radioimmunoassay for the measurement of lactoferrin in human plasma: variations with age, sex, and disease

R. M. Bennett, C. Mohla

    Research output: Contribution to journalArticle

    85 Scopus citations

    Abstract

    A solid phase radioimmunoassay is described for measuring lactoferrin levels in normal human plasma. The sensitivity of the assay was 6 ng. per milliliter with an intraassay coefficient of variation of 4 per cent and an interassay value of 9 per cent. Healthy adult males had a mean plasma level of 1.62 μg per milliliter which was significantly higher than adult females, 1.07 μg per milliliter. Postmenopausal females had levels similar to men, 1.74 μg per milliliter, while younger women had a significantly lower mean value, 0.75 μg per milliliter. Two menstruating women and 2 pregnant women had moderately elevated levels. Consistently elevated levels were found in patients with untreated or relapsing chronic myeloid leukemia, all over 12.0 μg per milliliter, while patients on marrow suppressant therapy tended to have subnormal levels. The collection of serum specimens as opposed to plasma, resulted in inconsistently elevated levels: EDTA was the anticoagulant of choice, as heparin interfered in the radioimmunoassay system.

    Original languageEnglish (US)
    Pages (from-to)156-166
    Number of pages11
    JournalJournal of Laboratory and Clinical Medicine
    Volume88
    Issue number1
    StatePublished - Dec 1 1976

    ASJC Scopus subject areas

    • Pathology and Forensic Medicine

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