A solid-phase assay for circulating immune complexes using monoclonal rheumatoid factors and peroxidase-linked protein A

Michael Davey, Leonhard Korngold

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

A solid-phase monoclonal rheumatoid factor (mRF) assay for circulating immune complexes (IC) is described. Microtiter plates are coated with mRF, serum diluted 1 : 4 is added, and complexed IgG is detected with protein A coupled to peroxidase. Selective binding was obtained with aggregated human gamma-globulin and soluble IC prepared in vitro. Sucrose density gradient ultracentrifugation studies indicate that mRF binds intermediate-size IC (between 7S and 19S). Abnormal levels of IC were found in 78% of sera from patients with rheumatoid arthritis, 67% with systemic lupus erythematosus, and 87% with subacute bacterial endocarditis.

Original languageEnglish (US)
Pages (from-to)87-100
Number of pages14
JournalJournal of Immunological Methods
Volume44
Issue number1
DOIs
StatePublished - Jul 17 1981
Externally publishedYes

Fingerprint

Rheumatoid Factor
Staphylococcal Protein A
Antigen-Antibody Complex
Peroxidase
Subacute Bacterial Endocarditis
gamma-Globulins
Ultracentrifugation
Serum
Systemic Lupus Erythematosus
Sucrose
Rheumatoid Arthritis
Immunoglobulin G

ASJC Scopus subject areas

  • Biotechnology
  • Immunology

Cite this

A solid-phase assay for circulating immune complexes using monoclonal rheumatoid factors and peroxidase-linked protein A. / Davey, Michael; Korngold, Leonhard.

In: Journal of Immunological Methods, Vol. 44, No. 1, 17.07.1981, p. 87-100.

Research output: Contribution to journalArticle

@article{43e007a0fc624231aaf4654ff986aa25,
title = "A solid-phase assay for circulating immune complexes using monoclonal rheumatoid factors and peroxidase-linked protein A",
abstract = "A solid-phase monoclonal rheumatoid factor (mRF) assay for circulating immune complexes (IC) is described. Microtiter plates are coated with mRF, serum diluted 1 : 4 is added, and complexed IgG is detected with protein A coupled to peroxidase. Selective binding was obtained with aggregated human gamma-globulin and soluble IC prepared in vitro. Sucrose density gradient ultracentrifugation studies indicate that mRF binds intermediate-size IC (between 7S and 19S). Abnormal levels of IC were found in 78{\%} of sera from patients with rheumatoid arthritis, 67{\%} with systemic lupus erythematosus, and 87{\%} with subacute bacterial endocarditis.",
author = "Michael Davey and Leonhard Korngold",
year = "1981",
month = "7",
day = "17",
doi = "10.1016/0022-1759(81)90110-1",
language = "English (US)",
volume = "44",
pages = "87--100",
journal = "Journal of Immunological Methods",
issn = "0022-1759",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - A solid-phase assay for circulating immune complexes using monoclonal rheumatoid factors and peroxidase-linked protein A

AU - Davey, Michael

AU - Korngold, Leonhard

PY - 1981/7/17

Y1 - 1981/7/17

N2 - A solid-phase monoclonal rheumatoid factor (mRF) assay for circulating immune complexes (IC) is described. Microtiter plates are coated with mRF, serum diluted 1 : 4 is added, and complexed IgG is detected with protein A coupled to peroxidase. Selective binding was obtained with aggregated human gamma-globulin and soluble IC prepared in vitro. Sucrose density gradient ultracentrifugation studies indicate that mRF binds intermediate-size IC (between 7S and 19S). Abnormal levels of IC were found in 78% of sera from patients with rheumatoid arthritis, 67% with systemic lupus erythematosus, and 87% with subacute bacterial endocarditis.

AB - A solid-phase monoclonal rheumatoid factor (mRF) assay for circulating immune complexes (IC) is described. Microtiter plates are coated with mRF, serum diluted 1 : 4 is added, and complexed IgG is detected with protein A coupled to peroxidase. Selective binding was obtained with aggregated human gamma-globulin and soluble IC prepared in vitro. Sucrose density gradient ultracentrifugation studies indicate that mRF binds intermediate-size IC (between 7S and 19S). Abnormal levels of IC were found in 78% of sera from patients with rheumatoid arthritis, 67% with systemic lupus erythematosus, and 87% with subacute bacterial endocarditis.

UR - http://www.scopus.com/inward/record.url?scp=0019500131&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019500131&partnerID=8YFLogxK

U2 - 10.1016/0022-1759(81)90110-1

DO - 10.1016/0022-1759(81)90110-1

M3 - Article

C2 - 7252177

AN - SCOPUS:0019500131

VL - 44

SP - 87

EP - 100

JO - Journal of Immunological Methods

JF - Journal of Immunological Methods

SN - 0022-1759

IS - 1

ER -