A solid-phase assay for circulating immune complexes using monoclonal rheumatoid factors and peroxidase-linked protein A

Michael P. Davey, Leonhard Korngold

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

A solid-phase monoclonal rheumatoid factor (mRF) assay for circulating immune complexes (IC) is described. Microtiter plates are coated with mRF, serum diluted 1 : 4 is added, and complexed IgG is detected with protein A coupled to peroxidase. Selective binding was obtained with aggregated human gamma-globulin and soluble IC prepared in vitro. Sucrose density gradient ultracentrifugation studies indicate that mRF binds intermediate-size IC (between 7S and 19S). Abnormal levels of IC were found in 78% of sera from patients with rheumatoid arthritis, 67% with systemic lupus erythematosus, and 87% with subacute bacterial endocarditis.

Original languageEnglish (US)
Pages (from-to)87-100
Number of pages14
JournalJournal of Immunological Methods
Volume44
Issue number1
DOIs
StatePublished - Jul 17 1981

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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