A sodium-dependent neutral-amino-acid transporter mediates infections of feline and baboon endogenous retroviruses and simian type D retroviruses

Chetankumar S. Tailor, Ali Nouri, Yuan Zhao, Yasuhiro Takeuchi, David Kabat

Research output: Contribution to journalArticle

122 Citations (Scopus)

Abstract

The type D simian retroviruses cause immunosuppression in macaques and have been reported as a presumptive opportunistic infection in a patient with AIDS. Previous evidence based on viral interference has strongly suggested that the type D simian viruses share a common but unknown cell surface receptor with three type C viruses: feline endogenous virus (RD114), baboon endogenous virus, and avian reticuloendotheliosis virus. Furthermore, the receptor gene for these viruses has been mapped to human chromosome 19q13.1- 13.2. We now report the isolation and characterization of a cell surface receptor for this group of retroviruses by using a human T-lymphocyte cDNA library in a retroviral vector. Swiss mouse fibroblasts (NIH 3T3), which are naturally resistant to RD114, were transduced with the retroviral library and then challenged with an RD114-pseudotyped virus containing a dominant selectable gene for puromycin resistance. Puromycin selection yielded 12 cellular clones that were highly susceptible to a β-galactosidase-encoding lacZ(RD114) pseudotype virus. Using PCR primers specific for vector sequences, we amplified a common 2.9-kb product from 10 positive clones. Expression of the 2.9-kb cDNA in Chinese hamster ovary cells conferred susceptibility to RD114, baboon endogenous virus, and the type D simian retroviruses. The 2.9-kb cDNA predicted a protein of 541 amino acids that had 98% identity with the previously cloned human Na+-dependent neutral-amino- acid transporter B°. Accordingly, expression of the RD114 receptor in NIH 3T3 cells resulted in enhanced cellular uptake of L-[3H]alanine and L- [3H]glutamine. RNA blot (Northern) analysis suggested that the RD114 receptor is widely expressed in human tissues and cell lines, including hematopoietic cells. The human B°transporter gene has been previously mapped to 19q13.3, which is closely linked to the gene locus of the RD114 receptor.

Original languageEnglish (US)
Pages (from-to)4470-4474
Number of pages5
JournalJournal of Virology
Volume73
Issue number5
StatePublished - 1999

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Simian Retroviruses
Retroviridae
Betaretrovirus
Neutral Amino Acid Transport Systems
amino acid transporters
Endogenous Retroviruses
Papio
Felidae
sodium
cats
Viruses
viruses
Infection
infection
receptors
Puromycin
puromycin
Cell Surface Receptors
Avian Reticuloendotheliosis Viruses
Viral Interference

ASJC Scopus subject areas

  • Immunology

Cite this

A sodium-dependent neutral-amino-acid transporter mediates infections of feline and baboon endogenous retroviruses and simian type D retroviruses. / Tailor, Chetankumar S.; Nouri, Ali; Zhao, Yuan; Takeuchi, Yasuhiro; Kabat, David.

In: Journal of Virology, Vol. 73, No. 5, 1999, p. 4470-4474.

Research output: Contribution to journalArticle

Tailor, Chetankumar S. ; Nouri, Ali ; Zhao, Yuan ; Takeuchi, Yasuhiro ; Kabat, David. / A sodium-dependent neutral-amino-acid transporter mediates infections of feline and baboon endogenous retroviruses and simian type D retroviruses. In: Journal of Virology. 1999 ; Vol. 73, No. 5. pp. 4470-4474.
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