A small-molecule FRET probe to monitor phospholipase A2 activity in cells and organisms

Oliver Wichmann, Jochen Wittbrodt, Carsten Schultz

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

(Chemical Equation Presented) Watching live: The FRET reporter PENN/SATE (see picture; FRET = fluorescence resonance energy transfer) is membrane permeant and enters cells and small organisms by using a prodrug approach. Once inside cells the reporter is cleaved by phospholipases, which results in a 30-fold change in the emission ratio.

Original languageEnglish (US)
Pages (from-to)508-512
Number of pages5
JournalAngewandte Chemie - International Edition
Volume45
Issue number3
DOIs
StatePublished - Jan 9 2006
Externally publishedYes

Fingerprint

Phospholipases
Phospholipases A2
Prodrugs
Membranes
Molecules
Fluorescence Resonance Energy Transfer

Keywords

  • Bioorganic chemistry
  • Fluorescent probes
  • FRET (fluorescence resonance energy transfer)
  • Phospholipids
  • Signal transduction

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

A small-molecule FRET probe to monitor phospholipase A2 activity in cells and organisms. / Wichmann, Oliver; Wittbrodt, Jochen; Schultz, Carsten.

In: Angewandte Chemie - International Edition, Vol. 45, No. 3, 09.01.2006, p. 508-512.

Research output: Contribution to journalArticle

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