A small-molecule FRET probe to monitor phospholipase A2 activity in cells and organisms

Oliver Wichmann; Jochen Wittbrodt; Carsten Schultz

doi:10.1002/anie.200500751

A small-molecule FRET probe to monitor phospholipase A² activity in cells and organisms

Oliver Wichmann, Jochen Wittbrodt, Carsten Schultz

Research output: Contribution to journal › Article › peer-review

52 Scopus citations

Abstract

(Chemical Equation Presented) Watching live: The FRET reporter PENN/SATE (see picture; FRET = fluorescence resonance energy transfer) is membrane permeant and enters cells and small organisms by using a prodrug approach. Once inside cells the reporter is cleaved by phospholipases, which results in a 30-fold change in the emission ratio.

Original language	English (US)
Pages (from-to)	508-512
Number of pages	5
Journal	Angewandte Chemie - International Edition
Volume	45
Issue number	3
DOIs	https://doi.org/10.1002/anie.200500751
State	Published - Jan 9 2006
Externally published	Yes

Keywords

Bioorganic chemistry
FRET (fluorescence resonance energy transfer)
Fluorescent probes
Phospholipids
Signal transduction

ASJC Scopus subject areas

Catalysis
General Chemistry

Access to Document

10.1002/anie.200500751

Cite this

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abstract = "(Chemical Equation Presented) Watching live: The FRET reporter PENN/SATE (see picture; FRET = fluorescence resonance energy transfer) is membrane permeant and enters cells and small organisms by using a prodrug approach. Once inside cells the reporter is cleaved by phospholipases, which results in a 30-fold change in the emission ratio.",

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AU - Wittbrodt, Jochen

AU - Schultz, Carsten

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KW - FRET (fluorescence resonance energy transfer)

KW - Fluorescent probes

KW - Phospholipids

KW - Signal transduction

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