A single receptor binds both insulin-like growth factor II and mannose-6-phosphate

Richard G. Macdonald, Suzanne R. Pfeffer, Lisa Coussens, Mark A. Tepper, Carol M. Brocklebank, John E. Mole, Jacqueline K. Anderson, Ellson Chen, Michael P. Czech, Axel Ullrich

Research output: Contribution to journalArticle

243 Citations (Scopus)

Abstract

Amino acid sequences deduced from rat complementary DNA clones encoding the insulin-like growth factor II(IGF-II) receptor closely resemble those of the bovine cation-independent mannose-6-phosphate receptor (Man-6-P receptor CI), suggesting they are identical structures. It is also shown that IGF-II receptors are adsorbed by immobilized pentamannosyl-6-phosphate and are specifically eluted with Man-6-P. Furthermore, Man-6-P specifically increases by about two times the apparent affinity of the purified rat placental receptor for 125I-labeled IGF-II. These results indicate that the type II IGF receptor contains cooperative, high-affinity binding sites for both IGF-II and Man-6-P-containing proteins.

Original languageEnglish (US)
Pages (from-to)1134-1137
Number of pages4
JournalScience
Volume239
Issue number4844
StatePublished - 1988
Externally publishedYes

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IGF Type 2 Receptor
Insulin-Like Growth Factor II
Cations
Amino Acid Sequence
Complementary DNA
Clone Cells
Phosphates
Binding Sites
mannose-6-phosphate
Proteins

ASJC Scopus subject areas

  • General

Cite this

Macdonald, R. G., Pfeffer, S. R., Coussens, L., Tepper, M. A., Brocklebank, C. M., Mole, J. E., ... Ullrich, A. (1988). A single receptor binds both insulin-like growth factor II and mannose-6-phosphate. Science, 239(4844), 1134-1137.

A single receptor binds both insulin-like growth factor II and mannose-6-phosphate. / Macdonald, Richard G.; Pfeffer, Suzanne R.; Coussens, Lisa; Tepper, Mark A.; Brocklebank, Carol M.; Mole, John E.; Anderson, Jacqueline K.; Chen, Ellson; Czech, Michael P.; Ullrich, Axel.

In: Science, Vol. 239, No. 4844, 1988, p. 1134-1137.

Research output: Contribution to journalArticle

Macdonald, RG, Pfeffer, SR, Coussens, L, Tepper, MA, Brocklebank, CM, Mole, JE, Anderson, JK, Chen, E, Czech, MP & Ullrich, A 1988, 'A single receptor binds both insulin-like growth factor II and mannose-6-phosphate', Science, vol. 239, no. 4844, pp. 1134-1137.
Macdonald RG, Pfeffer SR, Coussens L, Tepper MA, Brocklebank CM, Mole JE et al. A single receptor binds both insulin-like growth factor II and mannose-6-phosphate. Science. 1988;239(4844):1134-1137.
Macdonald, Richard G. ; Pfeffer, Suzanne R. ; Coussens, Lisa ; Tepper, Mark A. ; Brocklebank, Carol M. ; Mole, John E. ; Anderson, Jacqueline K. ; Chen, Ellson ; Czech, Michael P. ; Ullrich, Axel. / A single receptor binds both insulin-like growth factor II and mannose-6-phosphate. In: Science. 1988 ; Vol. 239, No. 4844. pp. 1134-1137.
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abstract = "Amino acid sequences deduced from rat complementary DNA clones encoding the insulin-like growth factor II(IGF-II) receptor closely resemble those of the bovine cation-independent mannose-6-phosphate receptor (Man-6-P receptor CI), suggesting they are identical structures. It is also shown that IGF-II receptors are adsorbed by immobilized pentamannosyl-6-phosphate and are specifically eluted with Man-6-P. Furthermore, Man-6-P specifically increases by about two times the apparent affinity of the purified rat placental receptor for 125I-labeled IGF-II. These results indicate that the type II IGF receptor contains cooperative, high-affinity binding sites for both IGF-II and Man-6-P-containing proteins.",
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