TY - JOUR
T1 - A single pulse of nerve growth factor triggers long-term neuronal excitability through sodium channel gene induction
AU - Toledo-Aral, Juan José
AU - Brehm, Paul
AU - Halegoua, Simon
AU - Mandel, Gail
N1 - Funding Information:
The authors acknowledge gratefully Dr. G. D'Arcangelo for sharing her unpublished data and Mr. Zhi-Jun He for help with Northern blot analysis. The work in this report was funded by grants from the NIH (to G. M., P. B., and S. H.) and by a grant from Trophix Pharmaceuticals, Inc. (to S. H.). J. J. T. A. is a postdoctoral fellow of the Spanish Ministerio de Educaci6n y Ciencia.
PY - 1995/3
Y1 - 1995/3
N2 - The continuous presence of nerve growth factor (NGF) is thought to be required for the elaboration of neuronal-like traits in PC12 cells. Surprisingly, we find that a 1 min exposure to NGF is sufficient to engage a longer-term genetic program leading to the acquisition of membrane excitability. Whereas continuous exposure to NGF causes the induction of a family of sodium channels, the effect of a brief exposure is to induce selectively expression of the peripheral nerve-type sodium channel gene PN1, through a distinct signaling pathway requiring immediate-early genes. A 1 min exposure of PC12 cells to interferon-γ also causes PN1 gene induction, suggesting that the "triggered" NGF and interferon-γ signaling pathways share common molecular intermediates.
AB - The continuous presence of nerve growth factor (NGF) is thought to be required for the elaboration of neuronal-like traits in PC12 cells. Surprisingly, we find that a 1 min exposure to NGF is sufficient to engage a longer-term genetic program leading to the acquisition of membrane excitability. Whereas continuous exposure to NGF causes the induction of a family of sodium channels, the effect of a brief exposure is to induce selectively expression of the peripheral nerve-type sodium channel gene PN1, through a distinct signaling pathway requiring immediate-early genes. A 1 min exposure of PC12 cells to interferon-γ also causes PN1 gene induction, suggesting that the "triggered" NGF and interferon-γ signaling pathways share common molecular intermediates.
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U2 - 10.1016/0896-6273(95)90317-8
DO - 10.1016/0896-6273(95)90317-8
M3 - Article
C2 - 7695907
AN - SCOPUS:0028947293
SN - 0896-6273
VL - 14
SP - 607
EP - 611
JO - Neuron
JF - Neuron
IS - 3
ER -