A single gp120 residue can affect HIV-1 tropism in macaques

Gregory Q. Del Prete, Brandon F. Keele, Jeannine Fode, Keyur Thumar, Adrienne E. Swanstrom, Anthony Rodriguez, Alice Raymond, Jacob Estes, Celia C. LaBranche, David C. Montefiori, Vineet N. KewalRamani, Jeffrey D. Lifson, Paul D. Bieniasz, Theodora Hatziioannou

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Species-dependent variation in proteins that aid or limit virus replication determines the ability of lentiviruses to jump between host species. Identifying and overcoming these differences facilitates the development of animal models for HIV-1, including models based on chimeric SIVs that express HIV-1 envelope (Env) glycoproteins, (SHIVs) and simian-tropic HIV-1 (stHIV) strains. Here, we demonstrate that the inherently poor ability of most HIV-1 Env proteins to use macaque CD4 as a receptor is improved during adaptation by virus passage in macaques. We identify a single amino acid, A281, in HIV-1 Env that consistently changes during adaptation in macaques and affects the ability of HIV-1 Env to use macaque CD4. Importantly, mutations at A281 do not markedly affect HIV-1 Env neutralization properties. Our findings should facilitate the design of HIV-1 Env proteins for use in non-human primate models and thus expedite the development of clinically relevant reagents for testing interventions against HIV-1.

Original languageEnglish (US)
Article numbere1006572
JournalPLoS Pathogens
Volume13
Issue number9
DOIs
StatePublished - Sep 1 2017
Externally publishedYes

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Tropism
Macaca
HIV-1
Lentivirus
Proteins
Virus Replication
Primates
Glycoproteins
Animal Models
Viruses
Amino Acids
Mutation

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Molecular Biology
  • Genetics
  • Virology

Cite this

Del Prete, G. Q., Keele, B. F., Fode, J., Thumar, K., Swanstrom, A. E., Rodriguez, A., ... Hatziioannou, T. (2017). A single gp120 residue can affect HIV-1 tropism in macaques. PLoS Pathogens, 13(9), [e1006572]. https://doi.org/10.1371/journal.ppat.1006572

A single gp120 residue can affect HIV-1 tropism in macaques. / Del Prete, Gregory Q.; Keele, Brandon F.; Fode, Jeannine; Thumar, Keyur; Swanstrom, Adrienne E.; Rodriguez, Anthony; Raymond, Alice; Estes, Jacob; LaBranche, Celia C.; Montefiori, David C.; KewalRamani, Vineet N.; Lifson, Jeffrey D.; Bieniasz, Paul D.; Hatziioannou, Theodora.

In: PLoS Pathogens, Vol. 13, No. 9, e1006572, 01.09.2017.

Research output: Contribution to journalArticle

Del Prete, GQ, Keele, BF, Fode, J, Thumar, K, Swanstrom, AE, Rodriguez, A, Raymond, A, Estes, J, LaBranche, CC, Montefiori, DC, KewalRamani, VN, Lifson, JD, Bieniasz, PD & Hatziioannou, T 2017, 'A single gp120 residue can affect HIV-1 tropism in macaques', PLoS Pathogens, vol. 13, no. 9, e1006572. https://doi.org/10.1371/journal.ppat.1006572
Del Prete GQ, Keele BF, Fode J, Thumar K, Swanstrom AE, Rodriguez A et al. A single gp120 residue can affect HIV-1 tropism in macaques. PLoS Pathogens. 2017 Sep 1;13(9). e1006572. https://doi.org/10.1371/journal.ppat.1006572
Del Prete, Gregory Q. ; Keele, Brandon F. ; Fode, Jeannine ; Thumar, Keyur ; Swanstrom, Adrienne E. ; Rodriguez, Anthony ; Raymond, Alice ; Estes, Jacob ; LaBranche, Celia C. ; Montefiori, David C. ; KewalRamani, Vineet N. ; Lifson, Jeffrey D. ; Bieniasz, Paul D. ; Hatziioannou, Theodora. / A single gp120 residue can affect HIV-1 tropism in macaques. In: PLoS Pathogens. 2017 ; Vol. 13, No. 9.
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