A simple method of mouse lung intubation.

Sandhya Das, Kelvin Macdonald, Herng Yu Sucie Chang, Wayne Mitzner

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

A simple procedure to intubate mice for pulmonary function measurements would have several advantages in longitudinal studies with limited numbers or expensive animal. One of the reasons that this is not done more routinely is that it is relatively difficult, despite there being several published studies that describe ways to achieve it. In this paper we demonstrate a procedure that eliminates one of the major hurdles associated with this intubation, that of visualizing the trachea during the entire time of intubation. The approach uses a 0.5 mm fiberoptic light source that serves as an introducer to direct the intubation cannula into the mouse trachea. We show that it is possible to use this procedure to measure lung mechanics in individual mice over a time course of at least several weeks. The technique can be set up with relatively little expense and expertise, and it can be routinely accomplished with relatively little training. This should make it possible for any laboratory to routinely carry out this intubation, thereby allowing longitudinal studies in individual mice, thereby minimizing the number of mice needed and increasing the statistical power by using each mouse as its own control.

Original languageEnglish (US)
JournalJournal of visualized experiments : JoVE
Issue number73
StatePublished - 2013
Externally publishedYes

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Intubation
Light sources
Mechanics
Animals
Lung
Trachea
Longitudinal Studies
Light

ASJC Scopus subject areas

  • Medicine(all)

Cite this

A simple method of mouse lung intubation. / Das, Sandhya; Macdonald, Kelvin; Chang, Herng Yu Sucie; Mitzner, Wayne.

In: Journal of visualized experiments : JoVE, No. 73, 2013.

Research output: Contribution to journalArticle

Das, Sandhya ; Macdonald, Kelvin ; Chang, Herng Yu Sucie ; Mitzner, Wayne. / A simple method of mouse lung intubation. In: Journal of visualized experiments : JoVE. 2013 ; No. 73.
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