A simple assay for proteolysis of IGFBP-3

George Lamson, Linda C. Giudice, Ronald (Ron) Rosenfeld

Research output: Contribution to journalArticle

158 Citations (Scopus)

Abstract

An assay for assessing the proteolysis of IGFBP-3 by a biological fluid or protein has been developed using 125I hIGFBP-3 recombinantly derived from Escherichia coli as a substrate indicator. The labelled substrate is incubated at 37° in the presence of the agent being tested and proleolysis is visualized by SDS-PAGE and autoradiography. The assay showed that the pregnancy associtated protease is Ca++ dependent. The substrute is also proteolyzed by rat and mouse pregnancy sera, making the assay applicable to studies in those animal systems.

Original languageEnglish (US)
Pages (from-to)1391-1393
Number of pages3
JournalJournal of Clinical Endocrinology and Metabolism
Volume72
Issue number6
StatePublished - Jun 1991
Externally publishedYes

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Proteolysis
Insulin-Like Growth Factor Binding Protein 3
Assays
Pregnancy
Autoradiography
Polyacrylamide Gel Electrophoresis
Peptide Hydrolases
Substrates
Escherichia coli
Rats
Animals
Serum
Proteins
Fluids

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this

Lamson, G., Giudice, L. C., & Rosenfeld, R. R. (1991). A simple assay for proteolysis of IGFBP-3. Journal of Clinical Endocrinology and Metabolism, 72(6), 1391-1393.

A simple assay for proteolysis of IGFBP-3. / Lamson, George; Giudice, Linda C.; Rosenfeld, Ronald (Ron).

In: Journal of Clinical Endocrinology and Metabolism, Vol. 72, No. 6, 06.1991, p. 1391-1393.

Research output: Contribution to journalArticle

Lamson, G, Giudice, LC & Rosenfeld, RR 1991, 'A simple assay for proteolysis of IGFBP-3', Journal of Clinical Endocrinology and Metabolism, vol. 72, no. 6, pp. 1391-1393.
Lamson G, Giudice LC, Rosenfeld RR. A simple assay for proteolysis of IGFBP-3. Journal of Clinical Endocrinology and Metabolism. 1991 Jun;72(6):1391-1393.
Lamson, George ; Giudice, Linda C. ; Rosenfeld, Ronald (Ron). / A simple assay for proteolysis of IGFBP-3. In: Journal of Clinical Endocrinology and Metabolism. 1991 ; Vol. 72, No. 6. pp. 1391-1393.
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