A simple assay for proteolysis of IGFBP-3

George Lamson; Linda C. Giudice; Ron G. Rosenfeld

doi:10.1210/jcem-72-6-1391

A simple assay for proteolysis of IGFBP-3

George Lamson, Linda C. Giudice, Ron G. Rosenfeld

Research output: Contribution to journal › Article › peer-review

164 Scopus citations

Abstract

An assay for assessing the proteolysis of IGFBP-3 by a biological fluid or protein has been developed using ¹²⁵I hIGFBP-3 recombinantly derived from Escherichia coli as a substrate indicator. The labelled substrate is incubated at 37° in the presence of the agent being tested and proteolysis is visualized by SDS-PAGE and autoradiography. The assay showed that the pregnancy associtatcd protease is Ca⁺⁺ dependent. The substrate is also proteolyed by rat and mouse pregnancy sera, making the assay applicable to studies in those animal systems.

Original language	English (US)
Pages (from-to)	1391-1393
Number of pages	3
Journal	Journal of Clinical Endocrinology and Metabolism
Volume	72
Issue number	6
DOIs	https://doi.org/10.1210/jcem-72-6-1391
State	Published - Jun 1991
Externally published	Yes

ASJC Scopus subject areas

Endocrinology, Diabetes and Metabolism
Biochemistry
Endocrinology
Clinical Biochemistry
Biochemistry, medical

Access to Document

10.1210/jcem-72-6-1391

Cite this

@article{33efacce8eff410c8a4749bb20410558,

title = "A simple assay for proteolysis of IGFBP-3",

abstract = "An assay for assessing the proteolysis of IGFBP-3 by a biological fluid or protein has been developed using 125I hIGFBP-3 recombinantly derived from Escherichia coli as a substrate indicator. The labelled substrate is incubated at 37° in the presence of the agent being tested and proteolysis is visualized by SDS-PAGE and autoradiography. The assay showed that the pregnancy associtatcd protease is Ca++ dependent. The substrate is also proteolyed by rat and mouse pregnancy sera, making the assay applicable to studies in those animal systems.",

author = "George Lamson and Giudice, {Linda C.} and Rosenfeld, {Ron G.}",

year = "1991",

month = jun,

doi = "10.1210/jcem-72-6-1391",

language = "English (US)",

volume = "72",

pages = "1391--1393",

journal = "Journal of Clinical Endocrinology and Metabolism",

issn = "0021-972X",

publisher = "The Endocrine Society",

number = "6",

}

TY - JOUR

T1 - A simple assay for proteolysis of IGFBP-3

AU - Lamson, George

AU - Giudice, Linda C.

AU - Rosenfeld, Ron G.

PY - 1991/6

Y1 - 1991/6

N2 - An assay for assessing the proteolysis of IGFBP-3 by a biological fluid or protein has been developed using 125I hIGFBP-3 recombinantly derived from Escherichia coli as a substrate indicator. The labelled substrate is incubated at 37° in the presence of the agent being tested and proteolysis is visualized by SDS-PAGE and autoradiography. The assay showed that the pregnancy associtatcd protease is Ca++ dependent. The substrate is also proteolyed by rat and mouse pregnancy sera, making the assay applicable to studies in those animal systems.

AB - An assay for assessing the proteolysis of IGFBP-3 by a biological fluid or protein has been developed using 125I hIGFBP-3 recombinantly derived from Escherichia coli as a substrate indicator. The labelled substrate is incubated at 37° in the presence of the agent being tested and proteolysis is visualized by SDS-PAGE and autoradiography. The assay showed that the pregnancy associtatcd protease is Ca++ dependent. The substrate is also proteolyed by rat and mouse pregnancy sera, making the assay applicable to studies in those animal systems.

UR - http://www.scopus.com/inward/record.url?scp=0025813304&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025813304&partnerID=8YFLogxK

U2 - 10.1210/jcem-72-6-1391

DO - 10.1210/jcem-72-6-1391

M3 - Article

C2 - 1709177

AN - SCOPUS:0025813304

VL - 72

SP - 1391

EP - 1393

JO - Journal of Clinical Endocrinology and Metabolism

JF - Journal of Clinical Endocrinology and Metabolism

SN - 0021-972X

IS - 6

ER -

A simple assay for proteolysis of IGFBP-3

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this