A simple assay for proteolysis of IGFBP-3

George Lamson, Linda C. Giudice, Ron G. Rosenfeld

Research output: Contribution to journalArticle

159 Scopus citations

Abstract

An assay for assessing the proteolysis of IGFBP-3 by a biological fluid or protein has been developed using 125I hIGFBP-3 recombinantly derived from Escherichia coli as a substrate indicator. The labelled substrate is incubated at 37° in the presence of the agent being tested and proteolysis is visualized by SDS-PAGE and autoradiography. The assay showed that the pregnancy associtatcd protease is Ca++ dependent. The substrate is also proteolyed by rat and mouse pregnancy sera, making the assay applicable to studies in those animal systems.

Original languageEnglish (US)
Pages (from-to)1391-1393
Number of pages3
JournalJournal of Clinical Endocrinology and Metabolism
Volume72
Issue number6
DOIs
StatePublished - Jun 1991

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Endocrinology
  • Clinical Biochemistry
  • Biochemistry, medical

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