Abstract
An assay for assessing the proteolysis of IGFBP-3 by a biological fluid or protein has been developed using 125I hIGFBP-3 recombinantly derived from Escherichia coli as a substrate indicator. The labelled substrate is incubated at 37° in the presence of the agent being tested and proleolysis is visualized by SDS-PAGE and autoradiography. The assay showed that the pregnancy associtated protease is Ca++ dependent. The substrute is also proteolyzed by rat and mouse pregnancy sera, making the assay applicable to studies in those animal systems.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1391-1393 |
| Number of pages | 3 |
| Journal | Journal of Clinical Endocrinology and Metabolism |
| Volume | 72 |
| Issue number | 6 |
| State | Published - Jun 1991 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biochemistry
- Endocrinology, Diabetes and Metabolism
Cite this
A simple assay for proteolysis of IGFBP-3. / Lamson, George; Giudice, Linda C.; Rosenfeld, Ronald (Ron).
In: Journal of Clinical Endocrinology and Metabolism, Vol. 72, No. 6, 06.1991, p. 1391-1393.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A simple assay for proteolysis of IGFBP-3
AU - Lamson, George
AU - Giudice, Linda C.
AU - Rosenfeld, Ronald (Ron)
PY - 1991/6
Y1 - 1991/6
N2 - An assay for assessing the proteolysis of IGFBP-3 by a biological fluid or protein has been developed using 125I hIGFBP-3 recombinantly derived from Escherichia coli as a substrate indicator. The labelled substrate is incubated at 37° in the presence of the agent being tested and proleolysis is visualized by SDS-PAGE and autoradiography. The assay showed that the pregnancy associtated protease is Ca++ dependent. The substrute is also proteolyzed by rat and mouse pregnancy sera, making the assay applicable to studies in those animal systems.
AB - An assay for assessing the proteolysis of IGFBP-3 by a biological fluid or protein has been developed using 125I hIGFBP-3 recombinantly derived from Escherichia coli as a substrate indicator. The labelled substrate is incubated at 37° in the presence of the agent being tested and proleolysis is visualized by SDS-PAGE and autoradiography. The assay showed that the pregnancy associtated protease is Ca++ dependent. The substrute is also proteolyzed by rat and mouse pregnancy sera, making the assay applicable to studies in those animal systems.
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UR - http://www.scopus.com/inward/citedby.url?scp=0025813304&partnerID=8YFLogxK
M3 - Article
VL - 72
SP - 1391
EP - 1393
JO - Journal of Clinical Endocrinology and Metabolism
JF - Journal of Clinical Endocrinology and Metabolism
SN - 0021-972X
IS - 6
ER -