Abstract
An assay for assessing the proteolysis of IGFBP-3 by a biological fluid or protein has been developed using 125I hIGFBP-3 recombinantly derived from Escherichia coli as a substrate indicator. The labelled substrate is incubated at 37° in the presence of the agent being tested and proteolysis is visualized by SDS-PAGE and autoradiography. The assay showed that the pregnancy associtatcd protease is Ca++ dependent. The substrate is also proteolyed by rat and mouse pregnancy sera, making the assay applicable to studies in those animal systems.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1391-1393 |
| Number of pages | 3 |
| Journal | Journal of Clinical Endocrinology and Metabolism |
| Volume | 72 |
| Issue number | 6 |
| DOIs | |
| State | Published - Jun 1991 |
ASJC Scopus subject areas
- Endocrinology, Diabetes and Metabolism
- Biochemistry
- Endocrinology
- Clinical Biochemistry
- Biochemistry, medical
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Lamson, G., Giudice, L. C., & Rosenfeld, R. G. (1991). A simple assay for proteolysis of IGFBP-3. Journal of Clinical Endocrinology and Metabolism, 72(6), 1391-1393. https://doi.org/10.1210/jcem-72-6-1391