A simple assay for proteolysis of IGFBP-3

George Lamson; Linda C. Giudice; Ron G. Rosenfeld

doi:10.1210/jcem-72-6-1391

A simple assay for proteolysis of IGFBP-3

George Lamson, Linda C. Giudice, Ron G. Rosenfeld

Pediatrics

Research output: Contribution to journal › Article › peer-review

159 Scopus citations

Abstract

An assay for assessing the proteolysis of IGFBP-3 by a biological fluid or protein has been developed using ¹²⁵I hIGFBP-3 recombinantly derived from Escherichia coli as a substrate indicator. The labelled substrate is incubated at 37° in the presence of the agent being tested and proteolysis is visualized by SDS-PAGE and autoradiography. The assay showed that the pregnancy associtatcd protease is Ca⁺⁺ dependent. The substrate is also proteolyed by rat and mouse pregnancy sera, making the assay applicable to studies in those animal systems.

Original language	English (US)
Pages (from-to)	1391-1393
Number of pages	3
Journal	Journal of Clinical Endocrinology and Metabolism
Volume	72
Issue number	6
DOIs	https://doi.org/10.1210/jcem-72-6-1391
State	Published - Jun 1991

ASJC Scopus subject areas

Endocrinology, Diabetes and Metabolism
Biochemistry
Endocrinology
Clinical Biochemistry
Biochemistry, medical

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