A Shared Mechanism for the Folding of Voltage-Gated K + Channels

Sarah K. McDonald, Talya S. Levitz, Francis Valiyaveetil

Research output: Contribution to journalArticle

Abstract

In this study, we probe the folding of K v AP, a voltage-gated K + (K v ) channel. The K v AP channel, though of archaebacterial origin, is structurally and functionally similar to eukaryotic K v channels. An advantage of the K v AP channel is that it can be folded in vitro from an extensively unfolded state and the folding can be controlled by temperature. We utilize these properties of the K v AP channel to separately study the membrane insertion and the tetramerization stages during folding. We use two quantitative assays: a Cys PEGylation assay to monitor membrane insertion and a cross-linking assay to monitor tetramerization. We show that during folding the K v AP polypeptide is rapidly inserted into the lipid bilayer with a "native-like" topology. We identify a segment at the C-terminus that is important for multimerization of the K v AP channel. We show that this C-terminal domain forms a dimer, which raises the possibility that the tetramerization of the K v AP channel proceeds through a dimer of dimers pathway. Our studies show that the in vitro folding of the K v AP channel mirrors aspects of the cellular assembly pathway for voltage-gated K + channels and therefore suggest that evolutionarily distinct K v channels share a common folding pathway. The pathway for the folding and assembly of a K v channel is of central importance as defects in this pathway have been implicated in the etiology of several disease states. Our studies indicate that the K v AP channel provides an experimentally tractable system for elucidating the folding mechanism of K v channels.

Original languageEnglish (US)
Pages (from-to)1660-1671
Number of pages12
JournalBiochemistry
Volume58
Issue number12
DOIs
StatePublished - Mar 26 2019

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Voltage-Gated Potassium Channels
Dimers
Assays
Membranes
Lipid Bilayers
Lipid bilayers
Peptides
Temperature
Topology
Defects
Electric potential
In Vitro Techniques

ASJC Scopus subject areas

  • Biochemistry

Cite this

A Shared Mechanism for the Folding of Voltage-Gated K + Channels . / McDonald, Sarah K.; Levitz, Talya S.; Valiyaveetil, Francis.

In: Biochemistry, Vol. 58, No. 12, 26.03.2019, p. 1660-1671.

Research output: Contribution to journalArticle

McDonald, Sarah K. ; Levitz, Talya S. ; Valiyaveetil, Francis. / A Shared Mechanism for the Folding of Voltage-Gated K + Channels In: Biochemistry. 2019 ; Vol. 58, No. 12. pp. 1660-1671.
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